Shoot Induction, Biochemical Changes During in vitro Rooting in Paeonia lactiflora Pall 'Hortensis'

2013 ◽  
Vol 1 (9) ◽  
pp. 318-324
Author(s):  
Byoung Ryong Jeong ◽  
Sonali Jana
Keyword(s):  
Biochemical Changes ◽  
In Vitro Rooting ◽  
Shoot Induction ◽  
Paeonia Lactiflora

Development of an Agrobacterium-mediated Transformation System for `Beurre Bosc' Pear

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461d-461
Author(s):  
Richard L. Bell ◽  
Ralph Scorza ◽  
Chinnathambi Srinivasan
Keyword(s):  
Shoot Proliferation ◽  
Induction Medium ◽  
Shoot Induction ◽  
Transformation System ◽  
Gus Histochemical Assay ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Shoot Induction Medium ◽  
Basal Salts

An efficient regeneration/transformation system was developed for `Beurre Bosc' pear. Young leaves were harvested from in vitro shoots proliferated on a medium containing MS basal salts and 5 BAP, 0.5 μM IBA, and 0.6M3. Shoot regeneration was optimized using a modification of the medium of Chevreau and Leblay (1993). Explants were cultured on shoot induction medium contained 10 μM TDZ and 1 μM IBA for 4 weeks in the dark, and then transfered to a similar, but auxinless, regeneration medium until shoots developed, usually after an additional 4 to 8 weeks. Leaf tissues were transformed by co-cultivation for 3 days with Agrobacterium tumefaciens EHA101 carrying a pGA482 plasmid containing NPTII, GUS, and rolC genes, followed by cultivation on SIM containing 300 mg/L timentin. Putative transgenic plants were selected on shoot induction medium containing 80mg/L kanamycin, and multiplied on shoot proliferation medium. Four clones were confirmed as transgenic using the GUS histochemical assay and Southern blots for the NPTII and rolC genes. Plants of each clone have been rooted and successfully transfered to the greenhouse for further analysis of gene expression.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Modeling and Optimizing a New Culture Medium for In Vitro Rooting of G×N15 Prunus Rootstock using Artificial Neural Network-Genetic Algorithm

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mohammad Mehdi Arab ◽  
Abbas Yadollahi ◽  
Maliheh Eftekhari ◽  
Hamed Ahmadi ◽  
Mohammad Akbari ◽  
...  
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Culture Medium ◽  
In Vitro Rooting ◽  
Artificial Neural

scholarly journals Light and Decapitation Effects on in Vitro Rooting in Maize Root Segments

1985 ◽  
Vol 79 (2) ◽  
pp. 377-380 ◽  
Author(s):  
Franck W. Golaz ◽  
Paul-Emile Pilet
Keyword(s):  
In Vitro Rooting ◽  
Maize Root ◽  
Root Segments

Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (Prunus amygdalus×P. persica)

2005 ◽  
Vol 106 (2) ◽  
pp. 268-272 ◽  
Author(s):  
Chrysovalantou Antonopoulou ◽  
Kortessa Dimassi ◽  
Ioannis Therios ◽  
Christos Chatzissavvidis ◽  
Vasilios Tsirakoglou
Keyword(s):  
Nutrient Concentration ◽  
In Vitro Rooting ◽  
Vitamin B2 ◽  
Inhibitory Effects ◽  
Prunus Amygdalus

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

Choice of Auxin for In Vitro Rooting of Apple Microcuttings

1997 ◽  
pp. 111-116 ◽  
Author(s):  
Geert-Jan De Klerk ◽  
Jolanda Ter Brugge ◽  
Jan Jasik ◽  
Svetla Marinova
Keyword(s):  
In Vitro Rooting
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