Verhoeff’s Iron Hematoxylin Staining v1 (protocols.io.be9sjh6e)

Two new substrate chromogens for alkaline phosphatase (ALP) detection have been recently synthesized for use in molecular biology research, salmon and magenta phosphate. We show here that these two chromogens have advantageous characteristics for immunocytochemistry as well. Their relatively delicate pink- and magenta-colored products do not mask the colors produced by other staining procedures. In addition, the reaction products of these substrates are insoluble in water, ethanol, and xylene, permitting the use of regressive hematoxylin staining procedures and coverslipping in permanent resin-based media. Most importantly, when these ALP substrates are used in double-label immunocytochemistry in combination with horseradish peroxidase-diaminobenzidine (HRP-DAB) and counterstained with hematoxylin, all three colors can be easily distinguished. An application using these substrates for simultaneous immunocytochemical detection of two monoclonal antibodies of different classes, in combination with hematoxylin staining, is illustrated.

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Development of a Murine Model of Pyogenic Flexor Tenosynovitis

10.1101/2020.02.07.925339 ◽

2020 ◽

Author(s):

Bowen Qiu ◽

Justin Cobb ◽

Alayna Loiselle ◽

Constantinos Ketonis

Keyword(s):

Murine Model ◽

Tendon Sheath ◽

Cellular Infiltrate ◽

Cellular Mechanisms ◽

Multiple Comparison Test ◽

Hematoxylin Staining ◽

Flexor Tendon Sheath ◽

Pyogenic Flexor Tenosynovitis ◽

Novel Therapeutic Strategies ◽

Flexor Tenosynovitis

ABSTRACTBackgroundTo demonstrate the plausibility of a murine model of pyogenic flexor tenosynovitis.Methods2μL of sterile PBS or bioluminescent Xen29 Staphylococcus aureus was administered to the tendon sheath of 36 male C57BL/6J mice. The infectious course was monitored by bioluminescence (BLI) signal via IVIS imaging and recording of weight change. The infected hind paws were harvested at four time points: 24 hours, 72 hours, 1 week and 2 weeks for histopathology using Alcian Blue hematoxylin staining. Two-way ANOVA with Sidak’s multiple comparison test was used for statistical analysis.ResultsThe infected cohort displayed significantly elevated bioluminescent values, reductions in weight, and exhibited swelling of the infected digit throughout the course of infection. By day 7 most infected mice saw a substantial decrease in BLI signal intensity, however two infected mice exhibited persistent BLI intensity through day 14. Histopathology of the infected cohort showed tissue disorganization and the presence of a cellular infiltrate in and around the flexor tendon sheath.ConclusionsA murine model of pyogenic flexor tenosynovitis is possible. Further optimization of the model offers an experimental platform for investigation of the pathophysiology of pyogenic flexor tenosynovitis.Clinical RelevanceThis animal model can be utilized in order to elucidate the basic molecular/cellular mechanisms of pyogenic flexor tenosynovitis while simultaneously evaluating novel therapeutic strategies.

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Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron

Microscopy and Microanalysis ◽

10.1017/s1431927619015058 ◽

2019 ◽

Vol 25 (6) ◽

pp. 1331-1340 ◽

Cited By ~ 2

Author(s):

Mario A. Gadan ◽

Rodrigo Lloyd ◽

Gisela Saint Martin ◽

María S. Olivera ◽

Lucía Policastro ◽

...

Keyword(s):

Cell Line ◽

Intracellular Localization ◽

Cancer Cell Line ◽

Boron Compounds ◽

Boron Neutron Capture ◽

Cell Structures ◽

Hematoxylin Staining ◽

Nuclear Tracks ◽

Clear Differentiation ◽

Uv C

AbstractOur group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.

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Hematoxylin Staining of Tissues Embedded in Epoxy Resins

Stain Technology ◽

10.3109/10520296909063319 ◽

1969 ◽

Vol 44 (1) ◽

pp. 21-25 ◽

Cited By ~ 16

Author(s):

T. K. Shires ◽

M. Johnson ◽

K. M. Richter

Keyword(s):

Epoxy Resins ◽

Hematoxylin Staining

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Concentration of Protozoan Cysts for Iron Hematoxylin Staining*

American Journal of Clinical Pathology ◽

10.1093/ajcp/19.1_ts.84 ◽

1949 ◽

Vol 19 (1_ts) ◽

pp. 84-85

Author(s):

Donald A. Johnson

Keyword(s):

Hematoxylin Staining

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METAL SALTS-HEMATOXYLIN STAINING OF SKIN KERATOHYALIN GRANULES

Journal of Histochemistry & Cytochemistry ◽

10.1177/15.2.104 ◽

1967 ◽

Vol 15 (2) ◽

pp. 104-110 ◽

Cited By ~ 18

Author(s):

PHILIP PIZZOLATO ◽

R. D. LILLIE

Keyword(s):

Metal Complexes ◽

Human Skin ◽

Metal Salts ◽

Distilled Water ◽

Stratum Granulosum ◽

Hematoxylin Staining ◽

Mammalian Skin ◽

Greenish Blue ◽

Dark Blue

The stratum granulosum of the mammalian skin contains keratohyalin granules which have been shown to have an affinity for salts of aluminum, chromium, copper and iron. To determine whether these granules have an affinity for other elements and whether the granule-metal complexes can be stained, salts of 64 metals were tested. Histologic sections of animal and human skin were treated with 0.01 M solution of various metal salts for 4 hr, washed in distilled water and stained with 1:10,000 dilution of unoxidized hematoxylin in 0.01 M phosphate butffer, pH 7.0, for 4 hr. In the cationic state, Al, Cr, Ga, Hf, In, Fe and Zr formed dark blue granules. Be, Dy, Ho, Ir, Pb, Mn, Mo, Nd, Ni, Pt, Rh, Tb, U, Yb and Zn produced lighter blue granules. The granules produced by Bi were purple; Ta, brown-red; Nb and Ti, brown; Cu, greenish blue; Sn and Th, purplish red; Os, blue- or green-brown. The metals were not extracted by washing in water up to 72 hr. Some metals were extracted by 1 N HCl and by 10% Versene.

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The Impact of Epstein Bar Virus On Hodgkin Lymphoma's Histopathological Patterns in Morocco

Blood ◽

10.1182/blood.v120.21.4781.4781 ◽

2012 ◽

Vol 120 (21) ◽

pp. 4781-4781

Author(s):

Soumaya Anoun ◽

Sofia Marouane ◽

Meryem Kabbali ◽

Soumia Zamiati ◽

Said Benchekroun ◽

...

Keyword(s):

Conflicts Of Interest ◽

Developed Countries ◽

Histological Subtype ◽

Male Predominance ◽

Barr Virus ◽

Ebv Infection ◽

Hematoxylin Staining ◽

Staining Protocol ◽

Epstein Barr ◽

The Impact

Abstract Abstract 4781 Background: In Morocco, improvement of living conditions and access to healthcare has increased life expectancy from 58 years in 1980 to 72 years in 2010. There has been a corresponding decrease in early childhood infections, including Epstein Barr virus (EBV) infection, which is associated with Hodgkin lymphoma (HL). Mixed cellularity (MC) was the most common histopathological subset of HL in Morocco. Prior studies have shown the shift to nodular sclerosis (NS) subset since 1998. Currently, the incidence of NS subtype is still predominant in our country. Our aim is to study the incidence of EBV infection in HL Moroccan population and correlate its decrease to explain the shift of HL histopathological pattern. Patients and methods: We compared children and adults with HL diagnosed during 3 periods: 1980–1995, 1998–2005 and 2010–2011 in the same university medical centre. All histological samples were prepared for examination by paraffin method, than stained by Hematoxylin staining protocol. Immunuhistochemical features of Hodgkin Reed Sternberg cells were performed to assess the expression of antibodies markers (CD30, CD15). The EBV profiling was identified by immunohistochemical stains for EBV latent membrane protein 1 (LMP-1) for the last periods. Statistical comparison of all the features was performed by Epi Info software. Results: The features of 1200 HL patients were enrolled. The average number of HL cases per year was 46 patients for the first period versus 74 patients for the last period. The mean age was respectively 24 years (range 2–64 years) for the first period, 32 years (range 2–60 years) for the second period and 34 years (range 3–76 years) for the third one. Sex-Ratio showed male predominance. The comparison of HL incidence through the age over the past 30 years is further characterized by a significant higher incidence in elderly patients (2% versus 10%), and lower incidence in young children (32% versus 7%). The expression of LMP1 in HRS is significantly reduced between the two last periods (P=0.001). LMP1 positivity is higher in patients under fifteen years than in young adults. Currently, NS is the most prevalent histological subtype with a significant increasing frequency through the three periods (p< 0.001) instead of MC subtype. (Table1). Conclusion: HL pattern in Morocco tends to be like in developed countries. The shift of HL histological subtype from CM to NS could be explained by the decrease of EBV infection in Hodgkin Reed Sternberg cells. More patients need to be enrolled to confirm this hypothesis Disclosures: No relevant conflicts of interest to declare.

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