First-strand synthesis and touchdown PCR for SARS-CoV-2 v2 (protocols.io.br54m88w)

The study was aimed to evaluate the prevalence of MRSA in some Iraqi hospitals and determine the most powerful methods for identification of MRSA, in order to achieve the, 278 samples were collected from different hospitals in Iraq in various intervals, 204 out of 287 were identified as Staphylococcus aureus by conventional cultural methods and microscopic characteristics and 177 isolates are identified as MRSA by using HiCrome MeReSa Agar Base medium, but 154 of 177 (87%) isolates are methicillin resistance in sensitivity test. MRSA isolates were highly resistant to β-lactam antibiotics and considered multidrug resistant (MDR) in percent of (94.9%). Touchdown PCR used to identify the isolates, 97.05% were identified as Staphylococcus aureus, while 80.88% as MRSA.

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Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles

Acta agriculturae Slovenica ◽

10.14720/aas.2018.111.1.14 ◽

2018 ◽

Vol 111 (1) ◽

pp. 143

Author(s):

Matjaž HLADNIK ◽

Jernej JAKŠE ◽

Bouchaib KHADARI ◽

Sylvain SANTONI ◽

Dunja BANDELJ

Keyword(s):

Interlaboratory Comparison ◽

Ficus Carica ◽

Reference Allele ◽

Discrimination Power ◽

Laboratory Equipment ◽

Allele Dropout ◽

Pcr Technology ◽

Touchdown Pcr ◽

Tail Pcr

Microsatellites have been identified as the marker of choice in plant genotyping projects. However, due to length discrepancies obtained between different laboratories for the same allele, interlaboratory comparison of fingerprinting results is often a difficult task. The objectives of this study were to compare genotyping results of two laboratories, to evaluate genetic parameters of microsatellite markers and to determine reference allele sizes for fig cultivars from the Istrian peninsula.Genotyping results of ninety fig (Ficus carica L.) accessions were comparable between the laboratories despite differences observed when comparing electropherograms of different capillary electrophoresis systems. Differences in lengths of the same alleles were detected due to different PCR methods and laboratory equipment, but the distances between alleles of the same locus were preserved. However, locus FSYC01 exhibited one allele dropout which led to misidentification of 28 heterozygotes as homozygote individuals suggesting this locus as unreliable. Allele dropout was assigned to the tail PCR technology or to a touchdown PCR protocol.Genotypes of twenty-four reference cultivars from the Istrian peninsula were confirmed by both laboratories. These results will contribute to the usage of markers with greater reliability, discrimination power and consequently, to more reliable standardization with other fig genotyping projects.

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Rapid Identification of Three Waterborne Pathogens in Water Samples by Multiplex Touchdown PCR

Proceedings of the 2015 International Conference on Mechanical Science and Engineering ◽

10.2991/mse-15.2016.33 ◽

2016 ◽

Author(s):

Yanjiao Yanjiao Hou ◽

Guanlin Guanlin Li ◽

Chen Chen Shao ◽

Shihe Shihe Shao

Keyword(s):

Water Samples ◽

Rapid Identification ◽

Waterborne Pathogens ◽

Touchdown Pcr

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Touchdown PCR for increased specificity and sensitivity in PCR amplification

Nature Protocols ◽

10.1038/nprot.2008.133 ◽

2008 ◽

Vol 3 (9) ◽

pp. 1452-1456 ◽

Cited By ~ 296

Author(s):

Darren J Korbie ◽

John S Mattick

Keyword(s):

Pcr Amplification ◽

Specificity And Sensitivity ◽

Touchdown Pcr

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Touchdown PCR

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg13371 ◽

2015 ◽

pp. 1-1

Keyword(s):

Touchdown Pcr

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Direct detection ofmecA,blaSHV, blaCTX-M,blaTEMandblaOXAgenes from positive blood culture bottles by multiplex-touchdown PCR assay

Letters in Applied Microbiology ◽

10.1111/lam.12676 ◽

2017 ◽

Vol 64 (2) ◽

pp. 138-143 ◽

Cited By ~ 3

Author(s):

M.-Y. Wang ◽

J.-L. Geng ◽

Y.-J. Chen ◽

Y. Song ◽

M. Sun ◽

...

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Direct Detection ◽

Pcr Assay ◽

Touchdown Pcr

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Chlamydia Pneumoniae in Asymptomatic Carotid Atherosclerosis

International Journal of Immunopathology and Pharmacology ◽

10.1177/205873920601900111 ◽

2006 ◽

Vol 19 (1) ◽

pp. 205873920601900 ◽

Cited By ~ 14

Author(s):

R. Sessa ◽

M. DI Pietro ◽

G. Schiavoni ◽

M. Galdiero ◽

P. Cipriani ◽

...

Keyword(s):

Chlamydia Pneumoniae ◽

Carotid Atherosclerosis ◽

Mononuclear Cells ◽

Asymptomatic Carotid ◽

Peripheral Blood Mononuclear ◽

Carotid Plaques ◽

First Choice ◽

Patients At Risk ◽

Touchdown Pcr ◽

Choice Method

We evaluated, in 415 patients with asymptomatic carotid atherosclerosis: (i) the prevalence of C. pneumoniae DNA in atherosclerotic carotid plaques and peripheral blood mononuclear cells (PBMC); (ii) the distribution of C. pneumoniae in atherosclerotic carotid plaques and PBMC from the same patients; (iii) the correlation between circulating anti-chlamydial antibodies and the presence of C. pneumoniae DNA. Overall, 160 atherosclerotic carotid plaques and 174 PBMC specimens from patients with asymptomatic carotid atherosclerosis were examined by ompA nested touchdown PCR for presence of C. pneumoniae. In addition, C. pneumoniae DNA was detected in 81 specimens of atherosclerotic carotid plaque and PBMC obtained from the same patients. C. pneumoniae DNA was found in 36.9% of atherosclerotic carotid plaques and in 40.2% of PBMC specimens examined (P=NS). With regard to 81 patients, C. pneumoniae DNA was detected in 27.2% of atherosclerotic carotid plaques and in 44.4% of PBMC specimens (P=0.05). In 18 patients, the presence of C. pneumoniae DNA in PBMC specimens and atherosclerotic carotid plaques coincided (P=0.005). No statistically significant association was found between anti-C pneumoniae antibodies (IgG and IgA) and positive PCR results. In conclusion, our results suggest that the detection of C. pneumoniae DNA in PBMC specimens seems to be a first-choice method to identify the patients at risk for endovascular chlamydial infection.

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