Analysis of Islet Function by Glucagon Enzyme-linked Immunosorbent Assay (ELISA) v1

2021 ◽  
Author(s):  
IIDP-HIPP not provided
Keyword(s):  
Kidney Diseases ◽  
Medical Center ◽  
Standard Operating Procedure ◽  
Human Islet ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Islet Function ◽  
Vanderbilt University ◽  
Qualitative Determination

This Standard Operating Procedure (SOP) is based on the Vanderbilt University Medical Center Human Islet Phenotyping Program (HIPP) Islet Functional Analysis. This SOP provides the HIPP procedure for measuring islet glucagon content and secretion to assess islet function. This SOP defines the assay method used by the Human Islet Phenotyping Program (HIPP) for the qualitative determination of the Purified Human Pancreatic Islet product, post-shipment, manufactured for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)-sponsored research in the Integrated Islet Distribution Program (IIDP). The goal of this SOP is to define the method for quantitative determination of glucagon released after secretagogue stimulation for proving the potency of the human islet preparation shipped by the IIDP.

Analysis of Islet Function by Insulin Enzyme-linked Immunosorbent Assay (ELISA) v1

2021 ◽  
Author(s):  
IIDP-HIPP not provided
Keyword(s):  
Kidney Diseases ◽  
Medical Center ◽  
Standard Operating Procedure ◽  
Human Islet ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Islet Function ◽  
Vanderbilt University ◽  
Qualitative Determination

This Standard Operating Procedure (SOP) is based on the Vanderbilt University Medical Center Human Islet Phenotyping Program (HIPP) Islet Functional Analysis. This SOP provides the HIPP procedure for measuring islet insulin content and secretion to assess islet function. This SOP defines the assay method used by the Human Islet Phenotyping Program (HIPP) for the qualitative determination of the Purified Human Pancreatic Islet product, post-shipment, manufactured for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)-sponsored research in the Integrated Islet Distribution Program (IIDP). The goal of this SOP is to define the method for quantitative determination of insulin released after glucose stimulation for proving the potency of the human islet preparation shipped by the IIDP.

Analysis of Islet Function in Dynamic Cell Perifusion System v1

2021 ◽  
Author(s):  
IIDP-HIPP not provided
Keyword(s):  
Kidney Diseases ◽  
Hormone Secretion ◽  
Standard Operating Procedure ◽  
Human Islet ◽  
Assay Method ◽  
Islet Function ◽  
Dynamic Cell ◽  
Perifusion System ◽  
Qualitative Determination

This Standard Operating Procedure (SOP) is based on the Vanderbilt Human Islet Phenotyping Program (HIPP) Islet Functional Analysis. This SOP provides HIPP procedure for dynamic perifusion and hormone secretion measurement to assess islet function. This SOP defines the assay method used by the Human Islet Phenotyping Program (HIPP) for qualitative determination of the Purified Human Pancreatic Islet product, post-shipment, manufactured for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)-sponsored research in the Integrated Islet Distribution Program (IIDP). The goal of this SOP is to define the method for quantitative determination of insulin released after glucose stimulation for proving the potency of the human islet preparation shipped by the IIDP.

Assessment of Human Islet Composition and Acinar Cell Component by Immunofluorescence Staining v2

2021 ◽  
Author(s):  
IIDP-HIPP not provided
Keyword(s):  
Kidney Diseases ◽  
Standard Operating Procedure ◽  
Human Islet ◽  
Immunofluorescence Staining ◽  
Immunofluorescent Staining ◽  
Assay Method ◽  
Staining Procedure ◽  
Cell Component ◽  
Qualitative Determination

This Standard Operating Procedure (SOP) is based on the Human Islet Phenotyping Program of the IIDP Immunofluorescence Staining Procedure. This SOP provides the HIPP procedure for immunofluorescent staining, imaging, and analysis of islet preparations. This SOP defines the assay method used by the Human Islet Phenotyping Program (HIPP) for quantitative and qualitative determination of the Purified Human Pancreatic Islet product, post-shipment, manufactured for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)-sponsored research in the Integrated Islet Distribution Program (IIDP).

Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method

Talanta ◽  
2013 ◽  
Vol 116 ◽  
pp. 343-346 ◽  
Author(s):  
Marina M. Vdovenko ◽  
Chun-Tse Hung ◽  
Ivan Yu. Sakharov ◽  
Feng-Yih Yu
Keyword(s):  
Immunosorbent Assay ◽  
Okadaic Acid ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Pharmacia ◽  
2020 ◽  
Vol 67 (4) ◽  
pp. 393-396
Author(s):  
Mykola V. Rybalkin ◽  
Natalya V. Khokhlenkova ◽  
Julia M. Azarenko ◽  
Tatiana V. Diadiun ◽  
Svitlana M. Kovalenko
Keyword(s):  
Candida Albicans ◽  
Therapeutic Effect ◽  
Immunosorbent Assay ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Protection ◽  
Specific Antibodies ◽  
Antibody Titers ◽  
Stimulation Of

Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.

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