Determination of Elution Conditions of Immunoaffinity Chromatography by ELISA (Enzyme Linked Immunosorbent Assay) Method

1992 ◽  
pp. 569-571
Author(s):  
Choe Taeboo ◽  
Chung Ilsun ◽  
Kim Jungkun
Talanta ◽  
2013 ◽  
Vol 116 ◽  
pp. 343-346 ◽  
Author(s):  
Marina M. Vdovenko ◽  
Chun-Tse Hung ◽  
Ivan Yu. Sakharov ◽  
Feng-Yih Yu

Pharmacia ◽  
2020 ◽  
Vol 67 (4) ◽  
pp. 393-396
Author(s):  
Mykola V. Rybalkin ◽  
Natalya V. Khokhlenkova ◽  
Julia M. Azarenko ◽  
Tatiana V. Diadiun ◽  
Svitlana M. Kovalenko

Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.


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