Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method

Talanta ◽  
2013 ◽  
Vol 116 ◽  
pp. 343-346 ◽  
Author(s):  
Marina M. Vdovenko ◽  
Chun-Tse Hung ◽  
Ivan Yu. Sakharov ◽  
Feng-Yih Yu
Keyword(s):  
Immunosorbent Assay ◽  
Okadaic Acid ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy

Pharmacia ◽  
2020 ◽  
Vol 67 (4) ◽  
pp. 393-396
Author(s):  
Mykola V. Rybalkin ◽  
Natalya V. Khokhlenkova ◽  
Julia M. Azarenko ◽  
Tatiana V. Diadiun ◽  
Svitlana M. Kovalenko
Keyword(s):  
Candida Albicans ◽  
Therapeutic Effect ◽  
Immunosorbent Assay ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Protection ◽  
Specific Antibodies ◽  
Antibody Titers ◽  
Stimulation Of

Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.

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