Sputum specimen processing for culture procedure v1 (protocols.io.uvzew76)

protocols.io ◽  
2018 ◽  
Author(s):  
Basra Doulla
2006 ◽  
Vol 16 (2) ◽  
pp. 86-92 ◽  
Author(s):  
Tiffany G. Harris ◽  
Howard D. Strickler ◽  
Herbert Yu ◽  
Michael N. Pollak ◽  
E. Scott Monrad ◽  
...  

2010 ◽  
Vol 201 (s1) ◽  
pp. S27-S36 ◽  
Author(s):  
Adrian Puren ◽  
Jay L. Gerlach ◽  
Bernhard H. Weigl ◽  
David M. Kelso ◽  
Gonzalo J. Domingo

2013 ◽  
Vol 189 (6) ◽  
pp. 2039-2046 ◽  
Author(s):  
Marc A. Bjurlin ◽  
H. Ballentine Carter ◽  
Paul Schellhammer ◽  
Michael S. Cookson ◽  
Leonard G. Gomella ◽  
...  

2019 ◽  
Vol 41 (3) ◽  
pp. 59-62
Author(s):  
Jyotshna Sapkota ◽  
Manisha Sharma ◽  
Deepti Shrestha ◽  
Beena Jha

Introduction Acinetobacter calcoaceticus-Acinetobacter baumanni (ACB) complex is one of the commonest cause of hospital acquired and ventilator associated pneumonia. Multidrug resistant Acinetobacter species have become a matter of huge concern. This study was done to find out the antibiotic susceptibility pattern of Acinetobacter calcoaceticus-Acinetobacter baumanii complex from sputum samples. MethodsThis descriptive cross-sectional study was carried out in Clinical Microbiology laboratory from July 2018 to Jan 2019 after ethical approval. Acinetobacter calcoaceticus-Acinetobacter baumannii complex was identified on the basis of its microscopy and morphological characteristics followed by biochemical tests. Antibiotic sensitivity test of isolated pathogens was done using Muller Hinton Agar by Kirby-Bauer method. ResultsOf the 384 culture positive sputum specimen, 76 (19.80%) were Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Most of the isolates were resistant to commonly used antibiotics, 72.36% of the isolates were multidrug resistance and 3.95% isolates were resistant to tigecycline. ConclusionThis study provides valuable information regarding prevalence of Acinetobacter calcoaceticus-Acinetobacter baumannii complex from sputum specimen. The alarming number of Multidrug resistance isolates is worrisome finding. Antibiotics like Tigecycline and Colistin which is still sensitive to isolates should be cautiously used only in MDR cases.


2016 ◽  
Vol 5 (3) ◽  
pp. 265-268
Author(s):  
Subramanyam Balaji ◽  
Vanaja Kumar ◽  
Prabu Seenivasan ◽  
R. Radhakrishnan ◽  
Gomathi Sekar ◽  
...  

2017 ◽  
Vol 43 (2) ◽  
pp. 324-348 ◽  
Author(s):  
Caitlin Donahue Wylie

New technologies can upset scientific workplaces’ established practices and social order. Scientists may therefore prefer preserving skilled manual work and the social status quo to revolutionary technological change. For example, digital imaging of rock-encased fossils is a valuable way for scientists to “see” a specimen without traditional rock removal. However, interviews in vertebrate paleontology laboratories reveal workers’ skepticism toward computed tomography (CT) imaging. Scientists criticize replacing physical fossils with digital images because, they say, images are more subjective than the “real thing.” I argue that these scientists are also implicitly supporting rock-removal technicians, who are skilled and trusted experts whose work would be made obsolete by widespread implementation of CT scanning. Scientists’ view of CT as a sometimes useful tool rather than a universal new approach to accessing fossils preserves the laboratory community’s social structure. Specifically, by privileging “real” specimens and trusted specimen-processing technicians over images and imaging experts, scientists preserve the lab community’s division of labor and skill, hierarchy between scientists and technicians, and these groups’ identity and mutual trust.


2015 ◽  
Vol 90 (4) ◽  
pp. 278-287 ◽  
Author(s):  
G Prats-Muñoz ◽  
A Malgosa ◽  
A Isidro ◽  
I Galtés

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