HUMAN PAPILLOMAVIRUS (HPV) INFECTION OF SEXUAL PARTNERS OF WOMEN PRESENTING CERVICAL INTRAEPITHELIAL NEOPLASIA

Human papillomavirus (HPV) infection is thought to be strongly associated with the precarcinomatous state cervical intraepithelial neoplasia (CIN) and cervical carcinoma. To accurately assess the correlation between HPV detection profiles and CIN, the uniplex E6/E7 polymerase chain reaction (PCR) method was used. We detected HPV (37 genotypes) in 267 CIN cases. The detection of a single high-risk HPV genotype occurred in 69.7% of CIN1 and worse than CIN1 (CIN1+) cases whereas other types were detected in 11.6% of cases. Codetection of high-risk HPV genotypes occurred in 4.9% of CIN1+ cases. The high-risk genotype HPV16 was the most frequently detected genotype in CIN1+ lesions; the genotype HPV34 (not a high-risk type) was detected in some CIN3 cases. Furthermore, HPV codetection may not be associated with CIN grades. These results suggest that various HPV genotypes are associated with CIN across all analyzed cases.

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Intralesional Beta-Interferon Treatment of Cervical Intraepithelial Neoplasia Associated with Human Papillomavirus Infection

Tumori Journal ◽

10.1177/030089169408000213 ◽

1994 ◽

Vol 80 (2) ◽

pp. 146-150 ◽

Cited By ~ 6

Author(s):

Carlo Penna ◽

Maria Grazia Fallani ◽

Rodolfo Gordigiani ◽

Lorella Sonni ◽

Gian Luigi Taddei ◽

...

Keyword(s):

Human Papillomavirus ◽

Side Effects ◽

Cervical Intraepithelial Neoplasia ◽

Treatment Options ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Cure Rate ◽

Papillomavirus Infection ◽

Before And After ◽

Ifn Therapy

Aims and backround Interferons (IFN) have offered considerable advances in the therapy of genital warts even those associated with cervical intraepithelial neoplasia (CIN); intralesional therapy either alone or in combination with other modalities such as cryosurgery and laser surgery provides improved clearing and cure of these often recalcitrant lesions. The purpose of this study was to evaluate the effectiveness of intralesional IFN therapy in patients with CIN associated with human papillomavirus (HPV) infection. Methods Beta-IFN was injected intra-perilesionally into the cervix in 41 patients with CIN associated with HPV infection. Results The regimen of 3 million international units (IU) injected intralesionally daily in the 1st week and 3 times a week in the 2nd and 3rd weeks for a total of 11 injections and a total dosage of 33 million IU yielded an 80 percent cure rate and may be more advantageous than other treatment options in certain instances. Cytocolposcopic and histologic examination was carried out before and after treatment and 24 lesions were also analyzed for type-specific papillomaviruses using in situ DNA hybridization. CIN disappeared in 33 patients 6 months after the end of therapy. Side effects of intralesional IFN therapy are dose related and for the most part readily tolerated. Conclusions Intralesional IFN proved to be effective treatment for CIN associated with HPV infection (cure rate: 80%) and well accepted because hospitalization is not required and no important side effects occur.

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Association between human papillomavirus in men and their sexual partners and uterine cervical intraepithelial neoplasia

International Urology and Nephrology ◽

10.1007/s11255-008-9428-6 ◽

2008 ◽

Vol 41 (2) ◽

pp. 335-340 ◽

Cited By ~ 5

Author(s):

José Guzmán-Esquivel ◽

Alicia Martínez-Contreras ◽

Mario Ramírez-Flores ◽

Lilia M. Jiménez Ceja ◽

Ivan Delgado-Enciso ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Sexual Partners

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Comprehensive genomic variation profiling of cervical intraepithelial neoplasia and cervical cancer identifies potential targets for cervical cancer early warning

Journal of Medical Genetics ◽

10.1136/jmedgenet-2018-105745 ◽

2018 ◽

Vol 56 (3) ◽

pp. 186-194 ◽

Cited By ~ 10

Author(s):

Jian Huang ◽

Zhaoyang Qian ◽

Yuhua Gong ◽

Yanzhou Wang ◽

Yanfang Guan ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Molecular Mechanisms ◽

Mutational Analysis ◽

Deep Understanding ◽

Genomic Analysis ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Genomic Variation

BackgroundTo better understand the pathogenesis of cervical cancer (CC), we systematically analysed the genomic variation and human papillomavirus (HPV) integration profiles of cervical intraepithelial neoplasia (CIN) and CC.MethodsWe performed whole-genome sequencing or whole-exome sequencing of 102 tumour-normal pairs and human papillomavirus probe capture sequencing of 45 CCs, 44 CIN samples and 25 normal cervical samples, and constructed strict integrated workflow of genomic analysis.ResultsMutational analysis identified eight significantly mutated genes in CC including four genes (FAT1, MLL3, MLL2 and FADD), which have not previously been reported in CC. Targetable alterations were identified in 55.9% of patients. In addition, HPV integration breakpoints occurred in 97.8% of the CC samples, 70.5% of the CIN samples and 42.8% of the normal cervical samples with HPV infection. Integrations of high-risk HPV strains in CCs, including HPV16, 18, 33 and 58, also occurred in the CIN samples. Moreover, gene mutations were detected in 52% of the CIN specimens, and 54.8% of these mutations occurred in genes that also mutated in CCs.ConclusionOur results lay the foundation for a deep understanding of the molecular mechanisms and finding new diagnostic and therapeutic targets of CC.

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Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

10.21203/rs.2.21014/v2 ◽

2020 ◽

Author(s):

Satoshi Baba ◽

Ayumi Taguchi ◽

Akira Kawata ◽

Konan Hara ◽

Satoko Eguchi ◽

...

Keyword(s):

Gene Expression ◽

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Detection Rate ◽

Expression Patterns ◽

Epidemiologic Study ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Hpv 16 ◽

Hpv Genotypes

Abstract Background Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6* , E1^E4 , and viral capsid protein L1 in four major HPV genotypes—HPV 16, 18, 52, and 58—were investigated. Results The detection rate of E6/E6* increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1 . Conclusions We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis.

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Penile lesions and human papillomavirus in male sexual partners of women with cervical intraepithelial neoplasia

Journal of the American Academy of Dermatology ◽

10.1067/mjd.2002.122198 ◽

2002 ◽

Vol 47 (3) ◽

pp. 351-357 ◽

Cited By ~ 62

Author(s):

Maaike C.G. Bleeker ◽

Cornelis J.A. Hogewoning ◽

Adriaan J.C. van den Brule ◽

Feja J. Voorhorst ◽

Rick E. van Andela ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Sexual Partners ◽

Male Sexual Partners

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HES1 Protein Modulates Human Papillomavirus–Mediated Carcinoma of the Uterine Cervix

Journal of Global Oncology ◽

10.1200/jgo.18.00141 ◽

2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Richa Tripathi ◽

Gayatri Rath ◽

Vishwas Sharma ◽

Showket Hussain ◽

Shashi Sharma ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Invasive Squamous Cell Carcinoma ◽

Hpv 16 ◽

Notch 3 ◽

Gynecology And Obstetrics ◽

Hes1 Expression ◽

Histologic Subtypes

PURPOSE Cervical cancer (CC) is the most common cancer affecting women worldwide. Human papillomavirus (HPV) infection is a major contributing factor for the development of CC. The development of CC occurs progressively from precancer stages to cancerous stages (ie, invasive squamous cell carcinoma [ISCC] and adenocarcinoma [ADC]). ADC is a rare form of CC that develops from the mucinous endocervical epithelium. It is believed that the downstream targets of Notch signaling contribute to the etiology of CC. One such target is HES1, whose role in the modulation of ADC is unknown. The purpose of this study is to determine the role of HES1 protein in HPV-associated ADC subtype of CC and also to compare its expression in histologic subtypes of precancer and ISCC. PATIENTS AND METHODS A total of 148 patients (30 with precancers, 98 with ISCC, and 20 with ADC) and 40 normal control participants were analyzed for the expression of HES1 via immunohistochemistry, with results validated by immunoblotting. RESULTS The comparison between HPV-16 and HES1 expression was significant in precancer (cervical intraepithelial neoplasia grades 1 to 3; P = .013), ISCC (International Federation of Gynecology and Obstetrics stages I to IV; P = .001), and ADC ( P = .007). An overall significant mean difference was observed between HES1, JAG1, and Notch-3 proteins in precancer ( P = .001), ISCC ( P = .001), and ADC ( P = .001). Pairwise comparisons between HES1 and JAG1 and HES1 and Notch-3 were also found to be significant. CONCLUSION This study showed that among all HPV-16–positive precancers, the major HES1 positivity signal arises from cervical intraepithelial neoplasia grades 2 and 3 that develops into ISCC. Moreover, HPV-16–positive ADC also showed an association with HES1. The HES1, JAG1, and Notch-3 proteins showed their synergistic role in modulating HPV associated ADC along with histologic subtypes of precancer and ISCC of CC.

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Distribution and clinical significance of human papillomavirus subtypes in Shenzhen city, People's Republic of China

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2007.01024.x ◽

2008 ◽

Vol 18 (2) ◽

pp. 295-299 ◽

Cited By ~ 6

Author(s):

Y. Dai ◽

Y. S. Huang ◽

M. Tang ◽

X. P. Lv ◽

T. Y. Li ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Blot Hybridization ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Multiple Infections ◽

Dot Blot ◽

Shenzhen City ◽

Hpv Status ◽

Malignant Changes

This study investigated the subtype distribution of the human papillomavirus (HPV) in the patients with condyloma accuminatum (CA) in Shenzhen city, China, and assessed the relationship between different HPV subtypes and cervical neoplasia. Type-specific prevalence and extent of multiple infections were assessed in the genital tract. CA samples collected from the 352 patients in the departments of dermatology and gynecology from the People's Hospital in Shenzhen during 2004–2006, using MY09/11 PCR and reverse dot blot hybridization for genotyping of 9–20 kinds of HPV subtypes. HPV status was studied in relation to the pathologic findings. HPV type diversity was wide. The low-risk HPV subtype 11 and 6 were the main subtypes, and multiple HPV infection rate was about 37% in HPV-positive samples. High-risk HPV (HR-HPV) types (16, 18, 58, 52, and 33) were the main subtypes in the CA of cervix, especially in the advanced stage cervical intraepithelial neoplasia (cervical intraepithelial neoplasia II+or above), multiple HR-HPV infection was found in 87% of HPV-positive samples. We conclude that HPV type 6 and 11 were the main subtypes in patients with CA in Shenzhen region, while HPV type 16 and 18 may be one of the main reasons for malignant changes of cervix, but this study cannot prove the association between multiple HPV infection and severity of cervix lesions.

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