scholarly journals Cellular and molecular aspects of degenerative disc disease and potential strategies of biological therapy

The Clinician ◽  
2020 ◽  
Vol 14 (1-2) ◽  
pp. 42-54
Author(s):  
A. V. Novikova ◽  
N. G. Pravdyuk ◽  
N. A. Shostak
Keyword(s):  
Degenerative Disc Disease ◽  
Biological Therapy ◽  
Viral Vector ◽  
Platelet Rich Plasma ◽  
Genetically Engineered ◽  
Biologically Active ◽  
Disc Disease ◽  
Plasma Therapy ◽  
Intercellular Substance ◽  
Degenerative Disc

Back pain is one of the main global health problems with a high level of prevalence and patients’ disability. In most cases, it is associated with degenerative spine damage (degenerative disc disease), dorsopathy, discopathy (M51 and M53 according to the International Classification of Diseases, 10th revision), affecting all levels of the intervertebral disc (IVD) (cytological, chemical and biochemical) as a whole as well as biological molecules that regulate homeostasis of the disc intercellular substance (growth factors, pro-inflammatory cytokines, enzymes). A key point in IVD dehydration is that catabolic processes predominate over anabolic ones due to changed gene expression in the corresponding biologically active molecules, disc angiogenesis and neoinnervation of the structures of the fibrous ring and pulpous nucleus. The latter is responsible for chronic pain in patients.Cells supporting homeostasis in nucleus pulpous, chondrocytes, continuously synthesize and restore proteoglycans and hyaluronic acid in nucleus pulpous, restoring shock-absorbing functions of the vertebral-motor segment. Decreased activity and death of chondrocytes in the avascular disc structure is a serious problem for reparative medicine. In accordance with IVD molecular-cellular mechanisms, numerous approaches to treat degenerative disc disease are being developed, each of which, influencing one of the links in the pathogenesis, has a direct or indirect effect on IVD repair.The article describes morphology, pathogenesis and genetics of degenerative disc disease, as well as main modern strategies of biological therapy: tissue engineering, biologically active substances locally used in IVD matrix, including PRP therapy (Platelet Rich Plasma therapy), methods of gene (using the viral vector) and cell therapy, as well as experience in the local use of genetically engineered biological products. Most successful studies are a combination of cell and gene therapy with the use of synthesized matrices.

A regenerative approach towards recovering the mechanical properties of degenerated intervertebral discs: Genipin and platelet-rich plasma therapies

2016 ◽  
Vol 231 (2) ◽  
pp. 127-137 ◽  
Author(s):  
Mohammad Nikkhoo ◽  
Jaw-Lin Wang ◽  
Masoud Abdollahi ◽  
Yu-Chun Hsu ◽  
Mohamad Parnianpour ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Material Properties ◽  
Degenerative Disc Disease ◽  
Structural Changes ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
Element Model ◽  
Intervertebral Discs ◽  
Disc Disease ◽  
Degenerative Disc

Degenerative disc disease, associated with discrete structural changes in the peripheral annulus and vertebral endplate, is one of the most common pathological triggers of acute and chronic low back pain, significantly depreciating an individual’s quality of life and instigating huge socioeconomic costs. Novel emerging therapeutic techniques are hence of great interest to both research and clinical communities alike. Exogenous crosslinking, such as Genipin, and platelet-rich plasma therapies have been recently demonstrated encouraging results for the repair and regeneration of degenerated discs, but there remains a knowledge gap regarding the quantitative degree of effectiveness and particular influence on the mechanical properties of the disc. This study aimed to investigate and quantify the material properties of intact (N = 8), trypsin-denatured (N = 8), Genipin-treated (N = 8), and platelet-rich plasma–treated (N = 8) discs in 32 porcine thoracic motion segments. A poroelastic finite element model was used to describe the mechanical properties during different treatments, while a meta-model analytical approach was used in combination with ex vivo experiments to extract the poroelastic material properties. The results revealed that both Genipin and platelet-rich plasma are able to recover the mechanical properties of denatured discs, thereby affording promising therapeutic modalities. However, platelet-rich plasma–treated discs fared slightly, but not significantly, better than Genipin in terms of recovering the glycosaminoglycans content, an essential building block for healthy discs. In addition to investigating these particular degenerative disc disease therapies, this study provides a systematic methodology for quantifying the detailed poroelastic mechanical properties of intervertebral disc.

scholarly journals Platelet-rich plasma effects on degenerative disc disease: analysis of histology and imaging in an animal model

2011 ◽  
Vol 2 (04) ◽  
pp. 13-18 ◽  
Author(s):  
Gregory Gullung ◽  
W Woodall ◽  
Michelle Tucci ◽  
Judy James ◽  
David Black ◽  
...  
Keyword(s):  
Animal Model ◽  
Degenerative Disc Disease ◽  
Platelet Rich Plasma ◽  
Disc Disease ◽  
Degenerative Disc ◽  
Plasma Effects ◽  
Disease Analysis

Hubungan Kadar Lipid Darah Dengan Degenerative Disc Disease Berdasarkan Klasifikasi Pfirrmann Menggunakan Magnetic Resonance Imaging Lumbosacral pada Pasien Nyeri Punggung Bawah

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Ryan Indra ◽  
Muhammad Ilyas ◽  
Mirna Muis ◽  
Bachtiar Murtala ◽  
Andi Alfian ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Degenerative Disc Disease ◽  
Serum Lipid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Resonance Imaging ◽  
Disc Disease ◽  
Degenerative Disc

Penelitian ini bertujuan mengetahui hubungan serum lipid darah dengan degenerative disc disease berdasarkan klasifikasi pfirrmann menggunakan magnetic resonance imaging lumbosacral pada pasien nyeri punggung bawah. Penelitian ini dilaksanakan di Departemen Radiologi RS. Dr. Wahidin Sudirohusodo Makassar mulai bulan Oktober 2018 sampai Januari 2019. Subjek sebanyak 52 orang dengan rentang usia 30 - 60 tahun. Metode yang digunakan adalah uji Spearman. Hasil penelitian menunjukkan terdapat korelasi antara kadar low-density lipoprotein (LDL) dan trigliserida serum dengan degenerative disc disease dengan nilai p=0.02 (p0.05). Semakin tinggi kadar LDL dan trigliserida maka derajat degerative disc disease cendereng semakin berat. Tidak terdapat korelasi antara HDL dengan degenerative disc disease. Secara statistik tidak terdapat korelasi antara kolesterol total dengan degenerative disc disease. Namun, didapatkan pada grafik nilai kolesterol total pada setiap derajat degenerative disc disease meningkat.

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