THE DETECTION OF PLUM POX VIRUS (PPV) BY INDICATOR PLANTS AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

1983 ◽  
pp. 151-160
Author(s):  
G. Hamdorf
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Indicator Plants

scholarly journals Detection of a New and Unusual Isolate of Plum pox virus in Plum (Prunus domestica)

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1119-1124 ◽  
Author(s):  
D. James ◽  
A. Varga ◽  
D. Thompson ◽  
S. Hayes
Keyword(s):  
Western Blot ◽  
Immunosorbent Assay ◽  
Molecular Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Polymorphism Analysis ◽  
Coding Region ◽  
Rt Pcr ◽  
Western Blot Assay ◽  
Restriction Sites

Plum pox virus (PPV) isolate 3174-01 was detected by triple-antibody sandwich enzyme-linked immunosorbent assay using the universal PPV monoclonal antibody (MAb) 5B as the secondary antibody, and by reverse-transcription polymerase chain reaction (RT-PCR) using primers that amplify a 243-bp fragment in the C-terminus of the coat protein coding region. The restriction sites RsaI and AluI were absent from this fragment, which is a feature unique to PPV-C isolates. The restriction sites in 3174-01 were replaced by GTAA/GTGA and GGCA, respectively. There was 95 to 99, 94, 91, and 92 to 94% identity of the 243-bp fragment of 3174-01 with the corresponding region of the strains C, D, EA, and M, respectively. Attempts to detect the virus by RT-PCR using strain C-specific primers in three different approaches were unsuccessful. All molecular techniques assessed in attempting to strain type isolate 3174-01 gave negative results, or results inconsistent for D or M in the case of P3-6K1 restriction fragment length polymorphism analysis. Isolate 3174-01 reacted in Western blot assay with MAb 5B, with an estimated molecular mass of 32 kDa. No reaction was observed with D-, M-, EA-, or C-specific monoclonal antibodies in Western blot or enzyme-linked immunosorbent assay. The molecular and serological data seem to indicate that PPV isolate 3174-01 does not belong to any of the recognized strains of PPV.

scholarly journals Evaluation of Resistance to Plum Pox Virus of North American and European Apricot Cultivars

HortScience ◽  
2003 ◽  
Vol 38 (4) ◽  
pp. 568-569 ◽  
Author(s):  
P. Martínez-Gómez ◽  
M. Rubio ◽  
F. Dicenta
Keyword(s):  
North America ◽  
Immunosorbent Assay ◽  
North American ◽  
Prunus Armeniaca ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Sharka Disease

The resistance to a Dideron isolate of Plum pox virus, which causes sharka disease, of four apricot (Prunus armeniaca L.) cultivars from North America (`Harlayne', `Henderson', `Sunglo', and `Veecot') and a Greek cultivar Lito (a cross of American cultivar Stark Early Orange × Greek cultivar Precoce Tirynthos) was evaluated. `Stark Early Orange' and `Canino', previously rated as resistant and susceptible respectively, were included as controls. Resistance, herein, was defined as inability to infect plants by graft-inoculation and negative assays by enzyme-linked immunosorbent assay. Cultivars found to be resistant were: `Harlayne', `Henderson', `Sunglo', `Lito', and `Stark Early Orange'. Cultivars Veecot and Canino were susceptible.

Identification of Viruses Infecting Peanut in Alabama1

1993 ◽  
Vol 20 (2) ◽  
pp. 71-73 ◽  
Author(s):  
R. T. Gudauskas ◽  
K. B. Burch ◽  
P. Jin ◽  
A. K. Hagan ◽  
J. R. Weeks
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indicator Plants ◽  
Tomato Spotted Wilt ◽  
Virus Incidence

Abstract Leaf samples collected from 1,883 peanut plants in 158 fields in 14 counties during July-August, 1990 and 1991, were tested for peanut mottle (PMV), peanut stripe (PStV), peanut stunt (PSV), and tomato spotted wilt (TSWV) viruses by sap inoculations onto indicator plants and/or by enzyme-linked immunosorbent assay (ELISA). Of 889 plants showing virus-like symptoms, 58% were infected with TSWV alone or mixed with PMV or PSV, 36% with PMV alone or mixed with TSWV or PSV, and 5% with PSV alone or mixed with TSWV or PMV. Double infections of PMV with TSWV, PSV with TSWV, and PMV with PSV were detected in 24%, 3%, and 1% of the symptomatic plants. Of 994 apparent asymptomatic plants, 16% were infected with PMV alone or mixed with TSWV, 8% with TSWV alone or mixed with PMV or PSV, and 5% with PSV alone or mixed with TSWV. Double infections of PMV with TSWV and PSV with TSWV were detected in 2% and 1% of the asymptomatic plants. PMV and TSWV were found in at least one field in every county sometime during the two seasons; PSV was found in 13 counties. The most notable difference in virus incidence between the two years was that TSWV was found in 20% of the asymptomatic plants in 1990 as compared to 6% in 1991. PStV was not detected by ELISA in 1990 or 1991, and no reactions suggestive of PStV or any virus other than PMV, PSV, or TSWV were observed on indicator plants used to assay the 1990 collections.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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