STUDIES ON SEVERAL STRAINS OF APPLE CHLOROTIC LEAF SPOT VIRUS (ACLSV) ISOLATED FROM DIFFERENT FRUIT TREE SPECIES.

1995 ◽  
pp. 63-71 ◽  
Author(s):  
M. Cieslinska ◽  
T. Malinowski ◽  
B.J. Zawadzka
Keyword(s):  
Tree Species ◽  
Leaf Spot ◽  
Fruit Tree ◽  
Spot Virus ◽  
Chlorotic Leaf

scholarly journals Mapping the RNA-binding domain on the Apple chlorotic leaf spot virus movement protein

2005 ◽  
Vol 86 (1) ◽  
pp. 225-229 ◽  
Author(s):  
Masamichi Isogai ◽  
Nobuyuki Yoshikawa
Keyword(s):  
Leaf Spot ◽  
Rna Binding ◽  
Movement Protein ◽  
Binding Domain ◽  
Sequence Specificity ◽  
Nucleic Acid Binding ◽  
Spot Virus ◽  
Uv Crosslinking ◽  
Chlorotic Leaf ◽  
Rna Binding Domain

The RNA-binding properties of the cell-to-cell movement protein (MP) of Apple chlorotic leaf spot virus were analysed. MP was expressed in Escherichia coli and was used in UV-crosslinking analysis, using a digoxigenin–UTP-labelled RNA probe and gel-retardation analysis. The analyses demonstrated that MP bound cooperatively to single-stranded RNA (ssRNA). When analysed for NaCl dependence of the RNA-binding activity, the majority of the MP could bind ssRNA even in binding buffer with 1 M NaCl. Furthermore, competition binding experiments showed that the MP bound preferentially to ssRNA and single-stranded DNA without sequence specificity. MP deletion mutants were used to identify the RNA-binding domain by UV-crosslinking analysis. Amino acid residues 82–126 and 127–287 potentially contain two independently active, single-stranded nucleic acid-binding domains.

Molecular Characterization and Distribution of Apple Chlorotic Leaf Spot Virus on Apple in China

2013 ◽  
Vol 162 (5) ◽  
pp. 284-290
Author(s):  
Hao Duan ◽  
Zhirui Ji ◽  
Shutong Wang ◽  
Tongle Hu ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Leaf Spot ◽  
Spot Virus ◽  
Chlorotic Leaf

scholarly journals Survey of Apple Chlorotic Leaf Spot Virus and Apple Stem Grooving Virus Occurrence in Korea and Frequency of Mixed Infections in Apple

2015 ◽  
Vol 60 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Jae-Yeong Han ◽  
Jung-Kyu Kim ◽  
Jin–Soo Cheong ◽  
Eun–Yeong Seo ◽  
Chan–Hwan Park ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Mixed Infections ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

CHAPTER 17: Apple chlorotic leaf spot virus in Stone Fruits

2011 ◽  
pp. 85-90 ◽  
Author(s):  
A. Myrta ◽  
S. Matic ◽  
T. Malinowski ◽  
G. Pasquini ◽  
T. Candresse
Keyword(s):  
Leaf Spot ◽  
Stone Fruits ◽  
Spot Virus ◽  
Chlorotic Leaf

Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

2010 ◽  
Vol 29 (12) ◽  
pp. 1447-1451 ◽  
Author(s):  
L.P. Wang ◽  
N. Hong ◽  
G.P. Wang ◽  
W.X. Xu ◽  
R. Michelutti ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf
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