DETECTION OF PRUNE DWARF VIRUS IN SWEET CHERRY IN ISRAEL

1998 ◽  
pp. 249-256 ◽  
Author(s):  
S. Spiegel ◽  
A. Rosner ◽  
Y. Tam ◽  
S. Zilkah ◽  
E. Faingersh ◽  
...  
Keyword(s):  
Sweet Cherry ◽  
Dwarf Virus ◽  
Prune Dwarf Virus

scholarly journals Molecular Identification of Prune Dwarf Virus (PDV) Infecting Sweet Cherry in Canada and Development of a PDV Full-Length Infectious cDNA Clone

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2025
Author(s):  
Aaron J. Simkovich ◽  
Yinzi Li ◽  
Susanne E. Kohalmi ◽  
Jonathan S. Griffiths ◽  
Aiming Wang
Keyword(s):  
Molecular Identification ◽  
Cdna Clone ◽  
Sweet Cherry ◽  
Infectious Clone ◽  
Infectious Cdna Clone ◽  
Dwarf Virus ◽  
Full Genome Sequence ◽  
Small Interfering Rnas ◽  
Prune Dwarf Virus ◽  
Fruit Species

Prune dwarf virus (PDV) is a member of ilarviruses that infects stone fruit species such as cherry, plum and peach, and ornamentally grown trees worldwide. The virus lacks an RNA silencing suppressor. Infection by PDV either alone, or its mixed infection with other viruses causes deteriorated fruit marketability and reduced fruit yields. Here, we report the molecular identification of PDV from sweet cherry in the prominent fruit growing region of Ontario, Canada known as the Niagara fruit belt using next generation sequencing of small interfering RNAs (siRNAs). We assessed its incidence in an experimental farm and determined the full genome sequence of this PDV isolate. We further constructed an infectious cDNA clone. Inoculation of the natural host cherry with this clone induced a dwarfing phenotype. We also examined its infectivity on several common experimental hosts. We found that it was infectious on cucurbits (cucumber and squash) with clear symptoms and Nicotiana benthamiana without causing noticeable symptoms, and it was unable to infect Arabidopsis thaliana. As generating infectious clones for woody plants is very challenging with limited success, the PDV infectious clone developed from this study will be a useful tool to facilitate molecular studies on PDV and related Prunus-infecting viruses.

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

2003 ◽  
Vol 114 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Genet Mekuria ◽  
Sunita A. Ramesh ◽  
Evita Alberts ◽  
Terry Bertozzi ◽  
Michelle Wirthensohn ◽  
...  
Keyword(s):  
Prunus Dulcis ◽  
Dwarf Virus ◽  
Rt Pcr ◽  
Spot Virus ◽  
Prune Dwarf Virus ◽  
Ring Spot

scholarly journals The Incidence and Genetic Diversity of Apple Mosaic Virus (ApMV) and Prune Dwarf Virus (PDV) in Prunus Species in Australia

Viruses ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 136 ◽  
Author(s):  
Wycliff Kinoti ◽  
Fiona Constable ◽  
Narelle Nancarrow ◽  
Kim Plummer ◽  
Brendan Rodoni
Keyword(s):  
Genetic Diversity ◽  
Mosaic Virus ◽  
Dwarf Virus ◽  
Prunus Species ◽  
Prune Dwarf Virus

scholarly journals Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 148 ◽  
Author(s):  
Edmund Kozieł ◽  
Katarzyna Otulak-Kozieł ◽  
Józef J. Bujarski
Keyword(s):  
Vascular Tissue ◽  
Chenopodium Quinoa ◽  
Cell Ultrastructure ◽  
Host Cells ◽  
Dwarf Virus ◽  
Ultrastructural Changes ◽  
Viral Pathogen ◽  
Prune Dwarf Virus ◽  
Systemic Spread ◽  
Specific Reactions

Prune dwarf virus (PDV) is a plant RNA viral pathogen in many orchard trees worldwide. Our knowledge about resistance genes or resistant reactions of plant hosts to PDV is scant. To fill in part of this gap, an aim of this study was to investigate reactions to PDV infection in a model host, Chenopodium quinoa. Our investigations concentrated on morphological and ultrastructural changes after inoculation with PDV strain 0599. It turned out that PDV infection can cause deformations in host cells but also induce changes in the organelles, such as chloroplasts in inoculated leaves. Moreover, we also demonstrated specific reactions/changes, which could be associated with both types of vascular tissue capable of effectively blocking the systemic spread of PDV to upper leaves. Furthermore, the relative amount of virus, P1 protein deposition, and movement protein (MP) gene expression consequently decreased in PDV-inoculated leaves.

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