IDENTIFICATION OF RAPD MARKERS LINKED TO LYCOPERSICON CHILENSE DERIVED BEGOMOVIRUS RESISTANT GENES ON CHROMOSOME 6 OF TOMATO

2005 ◽  
pp. 407-416 ◽  
Author(s):  
Y. Ji ◽  
J.W. Scott
Keyword(s):  
Rapd Markers ◽  
Chromosome 6 ◽  
Lycopersicon Chilense ◽  
Resistant Genes

Quantitative trait loci for heading date and straw characters in barley

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1098-1104 ◽  
Author(s):  
B. Kjær ◽  
J. Jensen ◽  
H. Giese
Keyword(s):  
Quantitative Trait Loci ◽  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Quantitative Trait ◽  
Rapd Markers ◽  
Spring Barley ◽  
Heading Date ◽  
Chromosome 6 ◽  
Chromosome 2 ◽  
Trait Loci

Quantitative trait loci (QTLs) for heading date and straw characters were examined in 79 chromosome-doubled haploid lines derived from the F1 generation of a cross between a six-rowed winter barley and a two-rowed spring barley. A genetic map covering 1100 cM containing 85 markers, including isozyme, morphological, RFLP, and RAPD markers, was constructed. All traits examined had two QTLs with large effects on chromosome 2. In addition, a QTL for length of the top internode was found on chromosome 6. The QTL in the chromosome segment around locus v (two row/six row) on chromosome 2 may be caused by pleiotropic effects of this locus. The same QTLs for heading date and straw length were found in both 1989 and 1991. The results indicate that two QTLs on chromosome 2 affect a group of correlated traits.Key words: Hordeum vulgare, earliness, RFLP, two rowed, six rowed, linkage map.

scholarly journals Integration of the classical and molecular linkage maps of tomato chromosome 6.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1175-1186
Author(s):  
R Weide ◽  
M F van Wordragen ◽  
R K Lankhorst ◽  
R Verkerk ◽  
C Hanhart ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
Linkage Data ◽  
Chromosome 6 ◽  
Morphological Markers ◽  
Limited Information ◽  
Tomato Genome ◽  
The Past ◽  
Molecular Linkage Map ◽  
Tomato Chromosome

Abstract In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited information about the relative positions of classical and molecular markers. In this paper we describe the construction of an integrated linkage map of tomato chromosome 6 that shows the position of cDNA-, genomic DNA- and RAPD markers relative to 10 classical markers. Integration was achieved by using a L. esculentum line containing an introgressed chromosome 6 from L. pennellii in crosses to a variety of L. esculentum marker lines. In addition, an improved version of the classical linkage map is presented that is based on a combined analysis of new linkage data for 16 morphological markers and literature data. Unlike the classical map currently in use, the revised map reveals clustering of markers into three major groups around the yv, m-2 and c loci, respectively. Although crossing-over rates are clearly different when comparing intraspecific L. esculentum crosses with L. esculentum x L. pennellii crosses, the clusters of morphological markers on the classical map coincide with clusters of genomic- and cDNA-markers on the molecular map constructed by Tanksley and coworkers.

scholarly journals (260) Development of SCAR and CAPS Markers Linked to Tomato Begomovirus Resistance Genes Introgressed from Lycopersicon chilense

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090A-1090 ◽  
Author(s):  
Yuanfu Ji ◽  
John W. Scott
Keyword(s):  
Resistance Genes ◽  
Rapd Markers ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Breeding Lines ◽  
Sequence Characterized Amplified Region ◽  
Lycopersicon Chilense ◽  
Caps Markers ◽  
Leaf Curl Virus

Resistance to begomoviruses tomato mottle virus (ToMoV) and tomato yellow leaf curl virus (TYLCV) has been introgressed to tomato (Lycopersicon esculentum) from L. chilense accessions LA 1932, LA 2779, and LA 1938. Resistance genes have been mapped to three regions on chromosome 6 using randomly amplified polymorphic DNA (RAPD) markers. We call these regions 1, 2, and 3. To facilitate breeding by marker assisted selection, advanced breeding lines with resistance from the above sources were assayed for the presence of RAPD markers to determine which were most tightly linked to begomovirus resistance. The best RAPD markers were then converted to sequence characterized amplified region (SCAR) markers or cleaved amplified polymorphic sequence (CAPS) markers. In addition, selected restriction fragment length polymorphism (RFLP) markers near the three regions were converted into CAPS markers, which were tested for association with the advanced breeding lines. Only LA 2779 derivatives have the L. chilense introgression in region 1, which is near the location of the Ty-1 gene and spans across CAPS markers 32.5Cla and TG118. Two region 1 RAPD markers UBC197 and UBC621 were converted co-dominant SCAR or CAPS markers, which were present in all 16 resistant breeding lines tested. Derivatives from all three accessions have introgressions in region 2. Further assays with more markers in this region are under way to determine the lengths and locations of the introgressions. No tightly linked RAPD markers have been found for the resistance gene from LA 1932 in region 3. RFLP and CAPS markers are being used to more precisely locate the region 3 gene.

scholarly journals Quantitative Trait Loci for Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Resistance in Tomato

2006 ◽  
Vol 131 (2) ◽  
pp. 267-272 ◽  
Author(s):  
H.A. Agrama ◽  
J.W. Scott
Keyword(s):  
Quantitative Trait Loci ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rapd Markers ◽  
Tomato Yellow Leaf ◽  
Mottle Virus ◽  
Chromosome 6 ◽  
Yellow Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

The genetic basis of resistance to tomato yellow leaf curl virus (TYLCV) and tomato mottle virus (ToMoV) was studied in three different mapping populations of tomato (Lycopersicon esculentum Mill.). Bulked segregant analysis (BSA) was used to identify random amplification of polymorphic DNA (RAPD) markers linked to TYLCV and ToMoV resistance. Segregated RAPD markers associated with resistance were linked to morphological markers self-pruning (sp) and potato leaf (c) on chromosome 6. RAPD genetic linkage maps of chromosome 6 were constructed for each of the three populations. Common mapped markers revealed straightforward homologies between the chromosome 6 linkage group of the three populations. Multiple-QTL mapping (MQM) was used to identify quantitative trait loci (QTL) for resistance linked to chromosome 6. These revealed that the resistance against TYLCV and ToMoV was mainly explained by two QTL in two populations and one QTL in another. For all of the resistance QTL detected, the favorable allele was provided by the resistant parents. The presence of three different sources of TYLCV and ToMoV resistance, and the markers in tight linkage with them, provide a means of systemically combining multiple resistance genes. Successful cloning of the R gene from tomatoes would lead to deeper understanding of the molecular basis of resistance to TYLCV and ToMoV, and might also shed light on the evolution of resistance genes in plants in general.

scholarly journals Inheritance and Linkage of Tomato Mottle Virus Resistance Genes Derived from Lycopersicon chilense Accession LA 1932

2001 ◽  
Vol 126 (4) ◽  
pp. 462-467 ◽  
Author(s):  
P.D. Griffiths ◽  
J.W. Scott
Keyword(s):  
Rapd Markers ◽  
Genetic Model ◽  
Mottle Virus ◽  
Potato Leaf ◽  
Lycopersicon Chilense ◽  
Generation Means ◽  
Generation Means Analysis ◽  
High Heritability ◽  
Susceptible Tomato ◽  
Additive Genes

Tomato mottle virus (ToMoV) is a silverleaf whitefly (Bemisia argentifolii Bellows and Perring n. sp.) transmitted, bipartite geminivirus that infects tomatoes (Lycopersicon esculentum Mill.). Inbred lines resistant to ToMoV were derived from Lycopersicon chilense Dunal accession LA 1932. Inheritance was studied using a family developed from the crossing of a resistant inbred with a susceptible tomato inbred over two seasons. The F1 had resistance intermediate to the parents and generation means analysis of F1 and F2, backcross and parental populations suggested that the action of at least two additive genes with high heritability (h2n.s. = 0.87) controlled ToMoV resistance. When data from the two seasons were combined, an acceptable fit to an additive-dominance genetic model was obtained. Single plant comparisons, bulk comparisons, and tailends of F2 populations segregating for ToMoV resistance derived from LA 1932 identified randomly amplified polymorphic DNA (RAPD) markers using eight hundred 10-mer oligonucleotide primers. The F2 populations used for inheritance studies were screened for polymorphic markers, and 12 RAPD markers associated with the ToMoV resistant line were linked to the morphological markers self-pruning (sp) and potato leaf (c) on chromosome 6. RAPD markers that were associated with ToMoV resistance segregated into two linked regions flanking either side of the sp and c loci. The molecular studies suggested that the action of at least two additive regions controlled ToMoV resistance which supported the inheritance analysis.

Identification of Molecular Markers Linked to Tomato Mottle Virus Resistance Genes

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 515b-515
Author(s):  
P.D. Griffiths ◽  
J.W. Scott
Keyword(s):  
Resistance Genes ◽  
Rapd Markers ◽  
Mottle Virus ◽  
Fresh Market ◽  
Arbitrary Primers ◽  
Breeding Lines ◽  
Lycopersicon Chilense ◽  
Good Repeatability ◽  
Tomato Breeding ◽  
Β Carotene

Tomato mottle virus (ToMoV) is a whitefly transmitted geminivirus threatening the Florida fresh-market tomato (Lycopersicon esculentum Mill.) industry. ToMoV resistance was identified in Lycopersicon chilense accessions LA 1932, LA 1938, LA 1969, and LA 2779, and introgressed into tomato breeding lines. Inheritance studies of populations derived from resistant accessions of LA 1932 and LA 1938 suggest that introgressed resistance is largely additive and multigenic. RAPD polymorphisms were identified using 800 arbitrary primers (decamers), and 88 polymorphisms with good repeatability were identified. Of these, 45 polymorphisms were identified in breeding lines derived from two or more of the four accessions. Analysis of segregating F2 populations has resulted in association of RAPD markers with geminivirus resistance genes, and markers have been identified that are common to different L. chilense resistance sources. Linkage of several markers to each other and to the self pruning (sp) and β-carotene (B) loci on chromosome 6 was also determined.

Deletion mapping on chromosome 6 in colorectal cancer associated with ulcerative colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A121-A122
Author(s):  
T EZAKI ◽  
M WATANABE ◽  
S FUNAKOSHI ◽  
M NAGANUMA ◽  
T AZUMA ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ulcerative Colitis ◽  
Deletion Mapping ◽  
Chromosome 6

Besondere Manifestationsformen der Autoimmunthyreopathie

Praxis ◽  
2002 ◽  
Vol 91 (27) ◽  
pp. 1151-1160
Author(s):  
Fajfr ◽  
Müller
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Chromosome 6 ◽  
Autoimmune Thyroid

Les maladies thyroïdiennes auto-immunes ou immunes (autoimmune thyroid disease, AITD) sont relativement fréquentes. Le terme de AITD comprend les thyréodites euthyroidiennes ou hypothyroïdiennes de Hashimoto avec ou sans goitre, les hyperthyroïdies classiques de Basedow et leurs variantes nettement plus rares euthyroïdiennes ou hypothyroïdiennes. Aucune des nombreuses classifications des AITD n'a pu s'imposer sur le plan international. La pathogénèse de toutes les formes d'AITD comprend une perturbation de la tolérance immune chez les individus prédisposés génétiquement (séquence HLA-DQAI*0501 sur le bras court du chromosome 6) qui provoque un processus auto-immun contre la glande thyroïdienne. Ces processus sont soit destructeurs ou inhibiteurs, soit stimulateurs, ce qui permet d'expliquer les formes très différentes de AITD. Dans de cas rares, ces processus peuvent se contrebalancer («balance hypotheseis»). Les anticorps anti-récepteurs TPO et TSH (TRAK) ont une place particulière dans le diagnostic des AITD. Les dosages de routine utilisent pour la mesure des TRAK des récepteurs qui ne peuvent pas différencier entre les anticorps stimulants ou bloquants contre les récepteurs TSH. C'est, entre autre pour ces raisons, que les résultats d'anticorps positifs ne sont utilisables qu'en connaissance de la clinique et / ou des paramètres de la fonction thyroïdienne. Ce travail présente quatre patients avec des formes plus complexes d'AITD et résume les connaissances actuelles.

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