CALLUS INİTİATİON FOR INDİRECT POLLEN EMBRYOGENESİS İN ANEMONE CORONARİA

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.

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Structural Changes During The Initiation Of Pollen Embryogenesis In Barley: Ultrastructure Analysis And Live Cell Imaging

Microscopy and Microanalysis ◽

10.1017/s1431927612003145 ◽

2012 ◽

Vol 18 (S2) ◽

pp. 258-259

Author(s):

M. Melzer

Keyword(s):

Live Cell Imaging ◽

Structural Changes ◽

Cell Imaging ◽

Live Cell ◽

Pollen Embryogenesis

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

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The effect of plant growth regulators on callus initiation in wormwood (Artemisia annua L.)

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v9i1.24 ◽

2016 ◽

Vol 9 (1) ◽

pp. 160 ◽

Cited By ~ 1

Author(s):

S.M. Tahir ◽

I. S. Usman ◽

M.D. Katung ◽

M. F. Ishiyaku

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Artemisia Annua ◽

Callus Initiation

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In vitro culture of switchgrass: Influence of 2,4-d and picloram in combination with benzyladenine on callus initiation and regeneration

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/bf00041117 ◽

1995 ◽

Vol 40 (1) ◽

pp. 43-48 ◽

Cited By ~ 24

Author(s):

P. D. Denchev ◽

B. V. Conger

Keyword(s):

In Vitro Culture ◽

Callus Initiation

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The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill.

Planta ◽

10.1007/bf00388396 ◽

1974 ◽

Vol 117 (3) ◽

pp. 227-241 ◽

Cited By ~ 85

Author(s):

N. Sunderland ◽

G. B. Collins ◽

J. M. Dunwell

Keyword(s):

Nuclear Fusion ◽

Pollen Embryogenesis ◽

Datura Innoxia

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MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

CBU International Conference Proceedings ◽

10.12955/cbup.v6.1313 ◽

2018 ◽

Vol 6 ◽

pp. 1185-1191

Author(s):

Minh Van Tran

Keyword(s):

Somatic Embryogenesis ◽

Somatic Cell ◽

Embryonic Cell ◽

Cell Suspensions ◽

Ms Medium ◽

Callus Initiation ◽

Set Up ◽

Planting Materials ◽

In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

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Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv ◽

10.7124/visnyk.utgis.17.1.1198 ◽

2019 ◽

Vol 17 (1) ◽

pp. 33-37

Author(s):

A. Z. Revutskaya ◽

A. V. Holubenko ◽

N. V. Nuzhyna ◽

H. O. Rudik ◽

N. Yu. Taran

Keyword(s):

In Vitro Culture ◽

Growth Regulators ◽

Nutrient Medium ◽

Callus Growth ◽

Basic Medium ◽

High Concentration ◽

Salvia Hispanica ◽

Callus Initiation ◽

Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

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PRIMING OF GENETIC VARIATIONS BY COLOCYNTH FRUIT EXTRACT AND CALLUS INITIATION IN EMBRYOS OF TWO CULTIVARS OF ALFALFA SEEDS

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i3.388 ◽

2017 ◽

Vol 48 (3) ◽

Author(s):

Hamza & Ali

Keyword(s):

Tissue Culture ◽

Dry Weight ◽

Culture Technique ◽

Factorial Experiment ◽

Graduate Studies ◽

College Of Agriculture ◽

Fruit Extract ◽

Local Variety ◽

Molecular Weights ◽

Callus Initiation

An experiment was conducted at Central lab of Graduate studies, College of Agriculture, University of Baghdad during 2015-2016. The aim was to study the effect of colocynth fruit extract on inducing callus from embryos of two alfalfa cultivars were PAC-78001 and local variety by tissue culture technique using factorial experiment within CRD. Seeds was soaked with colocynth fruit extract at 0, 75, 150, 225, and 300 ml Li-1 for 24 hours, then, sterilized by NaOCl at 4.5% for 15 minutes. The results showed significant differences between two cultivars responding to colocynth fruit extract levels. Concentration at 150 ml Li-1 gave the highest both fresh and dry weight of callus (205.90 and 20.60) respectively. Also, PCR results depending on RAPD and electrophoresis for DNA samples which isolated from callus of two cultivars and subjected to various concentrations of colocynth fruit extract 0, 75, 150, 225 and 300 ml Li-1 showed differences in amplified observed bands, molecular weights and brightness intensity by using primers primer -Y07, -Y06, OE-12, C-08, D-10, -Y10 and A-16, and There was no difference in bands number at all concentrations of colocynth fruit extract for local variety by primer A-16.

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