IN VITRO GERMINATION AND SEEDLING DEVELOPMENT OF CAPER (CAPPARIS SPINOSA L.) MATURE SEEDS

Nonstratified seeds of different genotypes of Fraxinusamericana L. (white ash), Fraxinuspennsylvanica Marsh, (green ash), and Fraxinusangustifolia Vahl. ssp. oxycarpa (narrowleaf ash, of Afghanistan origin) were surface disinfested, cut, and germinated in vitro on agar-solidified MS (Murashige and Skoog) medium containing sucrose and various plant growth regulators. Before placing in vitro, approximately one-third of the seed opposite the radicular end was excised and discarded, or the apical and basal 1 mm tips of the seed were aseptically excised. Within 1 week, viable seeds germinated. Cotyledons emerged, elongated, expanded, and turned green. After 2 weeks hypocotyls and radicles elongated. White and green ash genotypes germinated at different rates; generally >80% of seeds placed in vitro germinated. Genotype and the presence of thidiazuron in the medium had an effect on seedling development (hypocotyl and epicotyl elongation). Immature as well as nonstratified mature seeds germinated and grew. Epicotyls were excised, rooted, and established in the greenhouse.

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Phenology and seed germination of the Indian Screw Tree Helicteres isora L. (Malvales: Malvaceae)

Journal of Threatened Taxa ◽

10.11609/jott.3058.9.12.11040-11044 ◽

2017 ◽

Vol 9 (12) ◽

pp. 11040 ◽

Cited By ~ 2

Author(s):

Mariappan Muthukumar ◽

Thiruppathi Senthil Kumar ◽

Mandali Venkateswara Rao

Keyword(s):

Seed Viability ◽

Early Summer ◽

Seedling Development ◽

Ms Medium ◽

Seed Setting ◽

Flower Buds ◽

Helicteres Isora ◽

Half Strength ◽

Mature Seeds

Helicteres isora L. (Malvaceae), a medicinally important tree species used in Ayurveda as well as by folklore practitioners, is studied for its phenology, associated problems with the environment and anthropogenic effects. In Helicteres isora, flower buds are initiated by the end of July and flowering occurs during the middle of August to late October. Fruits mature during late January to early April. In between various factors like blister beetles, unidentified worms, deficit rainfall, early summer affect the flowering and seed setting, which cause 35–45 % loss. Seed viability of mature seeds were recorded 88.2% at fresh collection and decreases over period of time. In vitro, among the various treatments, acid treated seeds (H2SO4, 98.0%; 1 min) resulted in the best germination response of 84.5%. Sterile wet cotton soaked with sterile distilled water was found suitable for germination compared to other substratum, while half-strength MS medium with 2.0% sucrose was suitable for seedling development.

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In vitro germination and seedling development of two European orchid species, Himantoglossum jankae Somlyay, Kreutz & Óvári and Spiranthes spiralis (L.) Chevall.

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-019-09997-z ◽

2019 ◽

Vol 55 (4) ◽

pp. 380-391 ◽

Cited By ~ 2

Author(s):

Jovana Dulić ◽

Mirjana Ljubojević ◽

Vladislav Ognjanov ◽

Goran Barać ◽

Tamara Dulić

Keyword(s):

Seedling Development ◽

In Vitro Germination ◽

Orchid Species

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In Vitro Germination and Initial Seedling Development of Krantz Aloe by Smoke-saturated Water and Seed Imbibition

HortTechnology ◽

10.21273/horttech04643-20 ◽

2020 ◽

Vol 30 (5) ◽

pp. 619-623

Author(s):

Claudia A. Espinosa-Leal ◽

Silverio Garcia-Lara

Keyword(s):

Response Times ◽

Culture Media ◽

Seedling Development ◽

In Vitro Germination ◽

Wild Populations ◽

Active Ingredients ◽

Seed Imbibition ◽

Saturated Water ◽

Pharmaceutical Industries

Krantz aloe (Aloe arborescens) is one of two aloe species currently used for the extraction of active ingredients that can be useful in the cosmetic and pharmaceutical industries. However, krantz aloe plants have been frequently collected from the wild, which is threatening wild populations. In vitro tissue culture would allow the growth of krantz aloe under controlled conditions, optimizing the production of active ingredients without risk to wild populations. The establishment of cultures from krantz aloe plant explants has proved difficult as a result of the long response times of the explants and their release of polyphenols. Krantz aloe seeds are not commonly used as a means of propagation because of their low germination percentages. The objective of this study was to evaluate the effects of seed imbibition (SI) with water and the addition of smoke-saturated water (SSW) to the culture medium on the in vitro germination and initial seedling development of krantz aloe. Seeds were germinated in vitro in axenic conditions. The treatments used were the addition of 10% SSW to the culture media, SI, and a combination of both (10% SSW + SI). Daily germination was recorded and gross morphology was measured after 1 month of culture establishment. The maximum germination percentage (GP) was achieved when 10% SSW was in the medium (97.2%), followed by the combined application of 10% SSW + SI (96.6%), both of which were almost 30% greater and significantly different from that of the control (69.4%). SI had an 83.3% GP. No significant differences were observed among treatments in any of the observed development parameters (leaf and root length and number). Taken together, these findings show that the use of SSW improves the in vitro germination of krantz aloe without affecting seedling development, indicating preliminarily that SSW represents a useful in vitro germination promoter for this species.

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In vitro germination and seedling development of Posidonia oceanica

Aquatic Botany ◽

10.1016/s0304-3770(97)00017-x ◽

1998 ◽

Vol 60 (1) ◽

pp. 83-93 ◽

Cited By ~ 34

Author(s):

Elena Balestri ◽

Luigi Piazzi ◽

Francesco Cinelli

Keyword(s):

Posidonia Oceanica ◽

Seedling Development ◽

In Vitro Germination

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Promotion of Immature Seed Germination in Jacaranda mimosifolia

HortScience ◽

10.21273/hortsci.40.5.1485 ◽

2005 ◽

Vol 40 (5) ◽

pp. 1485-1486

Author(s):

Ikuo Miyajima ◽

Adriana Kato ◽

Juan Carlos Hagiwara ◽

Diego Mata ◽

Gabriela Facciuto ◽

...

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seedling Stage ◽

Immature Seed ◽

In Vitro Germination ◽

Immature Seeds ◽

Mature Seeds ◽

Jacaranda Mimosifolia

In vitro germination of immature seeds of Jacaranda mimosifolia treated with gibberellic acid (GA3) was studied. Immature seeds were collected monthly after crossings and sown on Murashige and Skoog (1962) medium with 3.0% sucrose and 0.6% agar after soaked 24 hours with 0, 10, 100, and 500 mg·L–1 GA3 solutions. Though germination was observed in the immature seeds harvested 2 months after crossing (2 MAC), the rate was quite low. When immature seeds of 3 MAC treated with 100 or 500 mg·L–1 GA3 solution were cultured, >60% germination were obtained within 2 weeks after culturing. These results indicate that immature seeds of 3 MAC treated with adequate GA3 solutions, seedlings can be obtained precociously and the period from crossing to the seedling stage was shorter than for mature seeds.

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