Development of efficient transformation systems for functional genomic research in apple

2016 ◽  
pp. 191-198
Author(s):  
H. Dai ◽  
W. Li ◽  
Z. Zhang
Keyword(s):  
Genomic Research ◽  
Functional Genomic ◽  
Efficient Transformation ◽  
Transformation Systems

scholarly journals Two highly representative rice BAC libraries of japonica cv Tainung 67 suitable for rice structural and functional genomic research

Plant Science ◽  
2006 ◽  
Vol 170 (4) ◽  
pp. 889-896
Author(s):  
Yann-Rong Lin ◽  
Teh-Yuan Chow ◽  
Meizhong Luo ◽  
Dave Kudrna ◽  
Chih-Chi Lin ◽  
...  
Keyword(s):  
Genomic Research ◽  
Functional Genomic ◽  
Bac Libraries

Boosting dsRNA delivery in plant and insect cells with peptide- and polymer-based carriers: case-based current status and future perspectives.

2021 ◽  
pp. 102-116
Author(s):  
Kristof de Schutter ◽  
Olivier Christiaens ◽  
Clauvis Nji Tizi Taning ◽  
Guy Smagghe
Keyword(s):  
Plant Cell Wall ◽  
Endosomal Escape ◽  
Genomic Research ◽  
Current Status ◽  
Functional Genomic ◽  
Future Perspectives ◽  
Agricultural Industry ◽  
Efficient Delivery ◽  
Rnai Response ◽  
Carrier Systems

Abstract Since the discovery of this naturally occurring endogenous regulatory and defence mechanism, RNA interference (RNAi) has been exploited as a powerful tool for functional genomic research. In addition, it has evolved as a promising candidate for a sustainable, specific and ecofriendly strategy for pest management and plant improvement. A key element in this technology is the efficient delivery of dsRNAs into the pest or plant tissues. While several examples using transgenic plants expressing the dsRNAs have proved the potential of this technology, nontransgenic approaches are investigated as alternatives, allowing flexibility and circumventing technical limitations of the transgenic approach. However, the efficacy of environmental RNAi is affected by several barriers, such as extracellular degradation of the dsRNA, inefficient internalization of the dsRNA in the cell and low endosomal escape into the cytoplasm, resulting in variable or low RNAi responses. In the medical field, carrier systems are commonly used to enhance RNA delivery and these systems are being rapidly adopted by the agricultural industry. Using four case studies, this chapter demonstrates the potential of carriers to improve the RNAi response in pest control for aquatic-living mosquito larvae and RNAi-resilient Lepidoptera and to cross the plant cell wall, allowing efficient environmental RNAi in plants.

scholarly journals Recent Progress in Functional Genomic Research in Plasmodium falciparum

2010 ◽  
Vol 11 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Jianbing Mu ◽  
Karl B. Seydel ◽  
Adam Bates ◽  
Xin-Zhuan Su
Keyword(s):  
Plasmodium Falciparum ◽  
Recent Progress ◽  
Genomic Research ◽  
Functional Genomic

Functional genomic resources for potato

2008 ◽  
Vol 88 (4) ◽  
pp. 573-581 ◽  
Author(s):  
Xiu-Qing Li ◽  
Rebecca Griffiths ◽  
David De Koeyer ◽  
Charlotte Rothwell ◽  
Vicki Gustafson ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Genetic Maps ◽  
Genomic Research ◽  
Functional Genomic ◽  
Entire Genome ◽  
Future Directions ◽  
Genomic Resources ◽  
Expressed Sequence ◽  
Selection Of ◽  
Made In

Considerable functional genomic resources have been developed by the potato research community in the past decade, including expressed sequence tag (EST) libraries, SAGE libraries, microarrays, molecular-function maps, and mutant populations. This article reviews the types, characteristics, strengths, limitations, and appropriate applications of these resources for genomic research and discusses perspectives on future directions. This wide selection of resources available to potato researchers complements efforts to sequence the entire genome and advances made in the development of saturated genetic maps. Key words: Solanum, potato, genomics, expressed sequence tag, microarray, longSAGE, data mining

Functional genomic research of α1-adrenoceptors

2002 ◽  
Vol 1244 ◽  
pp. 85-92
Author(s):  
Gozoh Tsujimoto ◽  
Susumu Katsuma ◽  
Satoshi Shiojima ◽  
Akira Hirasawa ◽  
Akito Tanoue
Keyword(s):  
Genomic Research ◽  
Functional Genomic

scholarly journals The Establishment of Two Efficient Transformation Systems in Quinoa

2021 ◽  
Author(s):  
Yanfang Wang ◽  
Yanzi Zhang ◽  
Chongjing Dai ◽  
Jun Ma ◽  
Yewei Zhou ◽  
...  
Keyword(s):  
Hairy Root ◽  
Stress Responses ◽  
Genetic Manipulation ◽  
Direct Injection ◽  
Chenopodium Quinoa ◽  
Free Food ◽  
Efficient Transformation ◽  
Low Efficiency ◽  
Transformation Systems

Abstract Quinoa (Chenopodium quinoa Willd.) provides a gluten-free food with abundant nutrition and is potential to become a major crop in future. Quinoa has a lot of unique features including the high tolerance to multiple stresses, of which the underlying mechanisms may help improve other crops. Genetic manipulation will provide powerful tools to investigate the function of key genes in regulation of quinoa development and stress responses, which will further improve quinoa planting in the fields. However, the efficient transformation system for quinoa has not been well developed yet. Here, we established two rapid and efficient transformation systems for quinoa by using hairy root and leaf agroinfiltration, which provide useful tools for quick analyses of genes function in quinoa. Hairy roots were obtained from three types of explants, including cotyledon-nod with hypocotyl, cotyledon itself, and hypocotyl pieces. Interestingly, explants of cotyledon-nod with hypocotyl showed the highest transformation efficiency at 67.9%, and cotyledon displayed medium efficiency at 42.2%, while hypocotyl with the lowest at 31.6%. We also obtained transgenic quinoa roots successfully in-vivo, which showed low efficiency, but this provides a potential method to test gene function in live plants. By using young leaves for agroinfiltration, direct injection showed a better transgenic effect compared with vacuum penetration. Overall, the transformation systems using both hairy root and leaf agroinfiltration provide efficient and convenient ways to manipulate and analyse gene functions in quinoa.

Functional Genomic Research of α1-Adrenoceptors

2001 ◽  
Vol 38 ◽  
pp. S1-S5
Author(s):  
Gozoh Tsujimoto ◽  
Susumu Katsuma ◽  
Satoshi Shiojima ◽  
Akira Hirasawa ◽  
Akito Tanoue
Keyword(s):  
Genomic Research ◽  
Functional Genomic

scholarly journals Sequencing of Euscaphis konishii Endocarp Transcriptome Points to Molecular Mechanisms of Endocarp Coloration

2018 ◽  
Vol 19 (10) ◽  
pp. 3209 ◽  
Author(s):  
Xueyan Yuan ◽  
Weihong Sun ◽  
Xiaoxing Zou ◽  
Bobin Liu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Anthocyanin Biosynthesis ◽  
Carotenoid Biosynthesis ◽  
Genomic Research ◽  
Functional Genomic ◽  
Glutathione S Transferase ◽  
Economic Market ◽  
Chlorophyll Breakdown ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

Flower and fruit colors are of vital importance to the ecology and economic market value of plants. The mechanisms of flower and fruit coloration have been well studied, especially among ornamental flower plants and cultivated fruits. As people pay more attention to exocarp coloration, the endocarp coloration in some species has often been ignored. Here, we report on the molecular mechanism of endocarp coloration in three development stages of Euscaphis konishii. The results show that endocarp reddening is closely related to anthocyanin accumulation, and a total of 86,120 unigenes were assembled, with a mean length of 893 bp (N50 length of 1642 bp). We identified a large number of differentially expressed genes associated with endocarp coloration, including anthocyanin biosynthesis, carotenoid biosynthesis, and chlorophyll breakdown. The genes participating in each step of the anthocyanin biosynthesis were found in the transcriptome dataset, but a few genes were found in the carotenoid biosynthesis and chlorophyll breakdown. In addition, the candidate R2R3-MYB transcription factors and candidate glutathione S-transferase transport genes, which likely regulate the anthocyanin biosynthesis, were identified. This study offers a platform for E. konishii functional genomic research and provides a reference for revealing the regulatory mechanisms of endocarp reddening.

Plant gene targeting and gene replacement: application to crop genetic improvement

2008 ◽  
Vol 5 (2) ◽  
pp. 93-99
Author(s):  
Zhang Xiao-hui ◽  
Ye Zhi-biao ◽  
Zhang Yu-yang ◽  
Hou Zheng ◽  
Li Han-xia
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
High Efficiency ◽  
Gene Replacement ◽  
Genomic Research ◽  
Functional Genomic ◽  
Molecular Modification ◽  
Base Replacement ◽  
Chimeric Oligonucleotides ◽  
Key Pathways

AbstractRecent advances and limitations of using zinc finger nuclease to stimulate a high efficiency of homologous recombination, and chimeric oligonucleotides to promote single base replacement in functional genomic research and plant genetic breeding, are systematically reviewed. Approaches to improve gene targeting efficiency through molecular modification of key pathways in plant homologous recombination are also discussed.

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