scholarly journals The role of polymorphisms genes of detoxification of xenobiotics in the development of endometriosis

2012 ◽  
Vol 61 (6) ◽  
pp. 18-24
Author(s):  
Natalya Vladimirovna Artymuk ◽  
Lyudmila Fedorovna Gulyayeva ◽  
Olga Aleksandrovna Zotova ◽  
Yekaterina Petrovna Khvostova
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Gene Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Estrogen Metabolism ◽  
Buccal Epithelium ◽  
Genotype C ◽  
Gene Cyp1a1 ◽  
Restriction Fragment Length

The review presents the results of investigations of gene polymorphism of enzymes involved in estrogen metabolism: CYP1A1, CYP1A2, CYP19 and SULT1A1 in the buccal epithelium samples from 703 women by RFLP (Restriction Fragment Length Polymorphism). The significant association of the presence of allele C and genotype T / C and C / C gene CYP1A1, allele A and genotype C / A and A / A CYP1A2 gene and the T allele and genotype C / T and C / C gene SYP19 with the risk of adenomyosis.

Adenomyosis: genetics of estrogen metabolism

2019 ◽  
Vol 37 (2) ◽  
Author(s):  
Natalia Artymuk ◽  
Olga Zotova ◽  
Lyudmila Gulyaeva
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Estrogen Metabolism ◽  
Control Group ◽  
Inclusion Criteria ◽  
Group I ◽  
Restriction Fragment Length

Abstract Background To analyze the allelic variants of genes of enzymes involved in estrogen metabolism: CYP1A1, CYP1A2, CYP19 and SULT1A1 using polymerase chain reaction-restriction fragment length polymorphism-restriction fragment length polymorphism (PCR-RFLP) analysis of women with histologically confirmed adenomyosis and women without proliferative diseases of pelvic organs was performed. We studied the following polymorphisms: CYP1A1 M1, T264 → C transition in the 3′-noncoding region; CYP1A2*1F, C734 → A transversion in CYP1A2 gene; C → T transition (Arg264Cys) in exon 7 of CYP19; SULT1A1*2, G638 → A transition (Arg213His) in the SULT1A1 gene. Materials and methods The study included 804 patients. Group I (experimental group) consisted of 268 women with adenomyosis. Inclusion criteria were: histological verification of adenomyosis, consent of patients to participate in the study. Group II (control group) – 536 women without proliferative diseases of the uterus. Inclusion criteria were: lack of proliferative processes of the uterus histologically confirmed by ultrasound examination, patient’s consent to participate in the study. Results We found the significant association of C allele, T/C and C/C genotypes of the CYP1A1 gene (CYP1A1 M1 polymorphism), A allele, C/A and A/A genotypes of the CYP1A2 gene (CYP1A2*1F polymorphism) and the T allele, C/T and C/C genotypes of the CYP19 (Arg264Cys polymorphism) gene with the risk for adenomyosis. Conclusions Patients with adenomyosis had increased frequency of C allele, T/C and C/C genotypes of the CYP1A1 gene, A allele, C/A and A/A genotypes of the CYP1A2 gene and T allele and C/T and C/C genotypes of the CYP19 gene and, on the contrary, decreased frequency of the mutant allele and heterozygous and mutant homozygous genotype of the CYP1A2 gene compared to women without proliferative diseases of the uterus.

scholarly journals Sources of Campylobacter spp. Colonizing Housed Broiler Flocks during Rearing

2006 ◽  
Vol 72 (1) ◽  
pp. 645-652 ◽  
Author(s):  
S. A. Bull ◽  
V. M. Allen ◽  
G. Domingue ◽  
F. Jørgensen ◽  
J. A. Frost ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Strong Evidence ◽  
Fragment Length Polymorphism ◽  
Growing Period ◽  
Broiler House ◽  
Campylobacter Spp ◽  
Restriction Fragment Length

ABSTRACT The study aimed to identify sources of campylobacter in 10 housed broiler flocks from three United Kingdom poultry companies. Samples from (i) the breeder flocks, which supplied the broilers, (ii) cleaned and disinfected houses prior to chick placement, (iii) the chickens, and (iv) the environments inside and outside the broiler houses during rearing were examined. Samples were collected at frequent intervals and examined for Campylobacter spp. Characterization of the isolates using multilocus sequence typing (MLST), serotyping, phage typing, and flaA restriction fragment length polymorphism typing was performed. Seven flocks became colonized during the growing period. Campylobacter spp. were detected in the environment surrounding the broiler house, prior to as well as during flock colonization, for six of these flocks. On two occasions, isolates detected in a puddle just prior to the birds being placed were indistinguishable from those colonizing the birds. Once flocks were colonized, indistinguishable strains of campylobacter were found in the feed and water and in the air of the broiler house. Campylobacter spp. were also detected in the air up to 30 m downstream of the broiler house, which raises the issue of the role of airborne transmission in the spread of campylobacter. At any time during rearing, broiler flocks were colonized by only one or two types determined by MLST but these changed, with some strains superseding others. In conclusion, the study provided strong evidence for the environment as a source of campylobacters colonizing housed broiler flocks. It also demonstrated colonization by successive campylobacter types determined by MLST during the life of a flock.

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