scholarly journals The effect of new quaternary phosphonium salts with highest alkyl substituents on the permeability of cell membranes for sodium ions in vitro

2012 ◽  
Vol 93 (3) ◽  
pp. 505-507
Author(s):  
O V Orlova ◽  
V N Oslopov ◽  
S A Sidullina
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Cell Membranes ◽  
Cell Membrane Permeability ◽  
Sodium Ions ◽  
Phosphonium Salts ◽  
In Vitro Methods ◽  
Quaternary Phosphonium Salts

Aim. To assess the effect of newly synthesized quaternary phosphonium salts with highest alkyl substituents (C10, C12, C14, C16, C18) on the permeability of cell membranes for sodium ions in vitro. Methods. In vitro studied was the effect of different concentrations of new quaternary phosphonium salts with highest alkyl substituents on the cell membrane permeability to sodium ions according to the rate of Na+-Li+-countertransport in the erythrocyte membrane. Results. Low concentration (0.001 mM) of substance C10 increased the rate of Na+-Li+-countertransport in the erythrocyte membrane by 11.9%, substance C12 - by 11.8%, substance C14 - by 12.7%, substance C16 - by 13%, substance C18 - by 12.3%. Higher concentrations of substances (0.01-0.05 mM) had no significant effect on the cell membrane permeability to sodium ions. Conclusion. An increase in the rate of Na+-Li+-countertransport in the erythrocyte membrane is influenced by minimal concentrations of a substance, under the influence of other concentrations the rate of Na+-Li+-countertransport doe not change.

scholarly journals Effects of triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide on cellular permeability in patients with hereditary cellular membrane hyperpermeability

2014 ◽  
Vol 95 (1) ◽  
pp. 59-62
Author(s):  
O V Orlova ◽  
V N Oslopov ◽  
S A Sidullina
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Biologically Active ◽  
Cell Membrane Permeability ◽  
Speed Increase ◽  
Antifungal Action ◽  
Antibacterial And Antifungal ◽  
Genetically Determined

Aim. Comparative analysis of effects of novel biologically active agents: triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide on cell membrane permeability for sodium by determination of of Na +-Li +-countertransport speed in erythrocyte membrane at patients with genetically determined high membrane permeability for sodium. Methods. Blood samples of 8 healthy volunteers who were classified as persons belonging to IV population quartile according to Na +-Li +-counter-transport speed in erythrocyte membrane, i.e. persons with high membrane permeability, were studied. Effects of different concentrations of triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide (known by antibacterial and antifungal action) on Na +-Li +-counter-transport speed in erythrocyte membrane in vitro according to the method proposed by M. Canessa et al. Results. Effect of triphenyltetradecylphosphonium bromide (С 14) and tributylhexadecylphosphonium bromide (С 16) on cell membrane permeability for sodium depends on the genetically determined baseline cell membrane state. С 14reduced the erythrocyte membrane permeability for sodium in studied patients belonging to IV quartile of Na +-Li +-counter-transport speed if administered in a concentration of 0.05 μm. C 16increased membrane permeability for sodium in the same group if administered in concentrations of 0.001 and 0.005 μm. Thus, tributylhexadecylphosphonium bromide is better suitable for designing a drug with antibacterial and antifungal action for patients belonging to IV quartile of Na +-Li +-counter-transport speed, if Na +-Li +-counter-transport speed reduction is wanted. If Na +-Li +-counter-transport speed increase is wanted, triphenyltetradecylphosphonium bromide is better suitable. Conclusion. Cand Csubstances affect cell membrane permeability for sodium in patients with genetically determined high membrane permeability for sodium.

Effect of Polycation Nanostructures on Cell Membrane Permeability and Toxicity

2022 ◽  
Author(s):  
Magdalena Wytrwal ◽  
Paulina Knobloch ◽  
Sławomir Lasota ◽  
Marta Michalik ◽  
M. Nowakowska ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membranes ◽  
Cell Membrane Permeability ◽  
Key Factors ◽  
Synthetic Materials

The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and...

scholarly journals Virulent Shigella flexneri Causes Damage to Mitochondria and Triggers Necrosis in Infected Human Monocyte-Derived Macrophages

2005 ◽  
Vol 73 (1) ◽  
pp. 504-513 ◽  
Author(s):  
James F. Koterski ◽  
Massoumeh Nahvi ◽  
Malabi M. Venkatesan ◽  
Beatrice Haimovich
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Shigella Flexneri ◽  
Human Monocyte ◽  
Cell Membrane Permeability ◽  
Vital Dyes

ABSTRACT Shigella flexneri is a gram-negative bacterium that causes bacillary dysentery in humans that is characterized by an acute inflammatory response of the colon. The fate of phagocytes that are infected in vitro with virulent Shigella has been the subject of some investigation and debate. In this study we found that virulent Shigella caused a rapid increase in the cell membrane permeability of infected human monocyte-derived macrophages (HMDM) but not in the cell membrane permeability of monocytes, as demonstrated by the uptake of fluorescent vital dyes. Within 2 h of infection, 59% ± 6% of the HMDM and ≤4% of the monocytes were stained with propidium iodide. Treatment of the cells with the inhibitors of caspases YVAD and zVAD, the antioxidants N-acetyl-l-cysteine and butylated hydroxyanisole, or an inhibitor of NADPH oxidase, diphenyleniodonium, did not alter the infection outcome. Importantly, we found that virulent Shigella caused a rapid drop in the ATP level to about 50% in infected HMDM. Furthermore, using a combination of fluorescent vital dyes and mitochondrial membrane potential-sensitive dyes, we observed that cells that exhibited a permeable cell membrane were not stained by the mitochondrion-specific dyes, indicating that the mitochondrial membrane potential was lost in these cells. We also observed infected cells that were not stained with either type of dye, indicating that the loss of the mitochondrial membrane potential preceded the increase in cell membrane permeability. Taken together, our studies showed that virulent Shigella flexneri targets the host cell mitochondria for destruction. This activity may account for the necrotic cell death precipitated by these pathogens.

scholarly journals Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effects

2020 ◽  
Vol 21 (24) ◽  
pp. 9756
Author(s):  
Zhaoxi Deng ◽  
Tian Dai ◽  
Wenming Zhang ◽  
Junli Zhu ◽  
Xin M. Luo ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Bacterial Cell ◽  
Lactobacillus Reuteri ◽  
Surface Proteins ◽  
Cell Membrane Permeability ◽  
In Vivo Studies ◽  
Bacterial Surface

The ability to adhere to the intestinal mucus layer is an important property of probiotic bacteria. Lactobacillus reuteri strains ZJ615 and ZJ617 show low and high adhesion, respectively, to intestinal epithelial cells. In this study, we quantified bacterial cell wall-associated glyceraldehyde-3-phosphate dehydrogenases (cw-GAPDH) and bacterial cell membrane permeability in both strains using immunoblotting and flow cytometry, respectively. Highly adhesive L. reuteri ZJ617 possessed significantly more cw-GAPDH, higher cell membrane permeability, and significantly higher adhesive ability toward mucin compared with low-adhesive L. reuteri ZJ615. In vitro adhesion studies and analysis of interaction kinetics using the Octet, the system revealed significantly decreased interaction between L. reuteri and mucin when mucin was oxidized when bacterial surface proteins were removed when bacteria were heat-inactivated at 80 °C for 30 min, and when the interaction was blocked with an anti-GAPDH antibody. SWISS-MODEL analysis suggested intensive interactions between mucin glycans (GalNAcα1-O-Ser, GalNAcαSer, and Galβ3GalNAc) and GAPDH. Furthermore, in vivo studies revealed significantly higher numbers of bacteria adhering to the jejunum, ileum, and colon of piglets orally inoculated with L. reuteri ZJ617 compared with those inoculated with L. reuteri ZJ615; this led to a significantly decreased rate of diarrhea in piglets inoculated with L. reuteri ZJ617. In conclusion, there are strong correlations among the abundance of cw-GAPDH in L. reuteri, the ability of the bacterium to adhere to the host, and the health benefits of this probiotic.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

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