scholarly journals Bacteriostatic Characteristics of Bone Substituting Constructors Obtained from Composite Materials Based on Natural Polymers, Calcium Phosphates and Vancomycin

2017 ◽  
Vol 24 (2) ◽  
pp. 48-56
Author(s):  
P. A Karalkin ◽  
N. S Sergeeva ◽  
V. S Komlev ◽  
I. K Sviridova ◽  
V. A Kirsanova ◽  
...  
Keyword(s):  
3D Printing ◽  
Calcium Phosphates ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Skin Defect ◽  
Natural Polymers ◽  
Electron Microscopic ◽  
Study Results ◽  
Microscopic Evaluation

Introduction. The local delivery of therapeutic antibiotic concentrations to the zone of surgical intervention in bone pathology enables either to prevent or significantly decrease the rate of osteomyelitis development. It that study the efficacy of vancomycin inclusion and release from three dimensional constructors based on sodium alginate, gelatin and octacalcium phosphate and vancomycin intended for bone defect substitution was studied. Materials and methods . Prototyping of 3D constructors was performed by extrusion 3D printing. Various concentrations of vancomycin were added as an additional component at the stage of preparation of hydrogel (“ink”) for printing. Physical testing of the constructors included electron microscopic evaluation of their microstructure and porosity as well as the study of mechanical strength for compression and stretching. Functional activity of printed constructors with respect to the test strain S. aureus ATCC 6538-P was assessed by a disc-diffusion method in vitro and on the model of infected excision skin wound in rats - in vivo. Results . Analysis of the kinetic curves showed that the principal release of the drug in the model liquid took place within the first day. The total volume of the bound and released vancomycin made up approximately 20% of the calculated initial amount for all three concentrations. Formation of the marked growth inhibition zone of S. aureus strain in presence of vancomycin rich constructors was demonstrated in vitro. Insertion of constructors into the zone of infected skin defect resulted in a decrease of inflammatory processes severity and rate as well as accelerated the terms of complete wound healing. Conclusion. Study results showed the principle potentiality of bone substituting implants 3D-printing using multicomponent hydrogel compositions without change of separate components characteristics.

scholarly journals Bacteriostatic Characteristics of Bone Substituting Constructors Obtained from Composite Materials Based on Natural Polymers, Calcium Phosphates and Vancomycin

2017 ◽  
pp. 48-56
Author(s):  
P. A. Karalkin ◽  
N. S. Sergeeva ◽  
V. S. Komlev ◽  
I. K. Sviridova ◽  
V. A. Kirsanova ◽  
...  
Keyword(s):  
3D Printing ◽  
Calcium Phosphates ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Skin Defect ◽  
Natural Polymers ◽  
Electron Microscopic ◽  
Study Results ◽  
Microscopic Evaluation

Introduction.The local delivery of therapeutic antibiotic concentrations to the zone of surgical intervention in bone pathology enables either to prevent or significantly decrease the rate of osteomyelitis development. It that study the efficacy of vancomycin inclusion and release from three dimensional constructors based on sodium alginate, gelatin and octacalcium phosphate and vancomycin intended for bone defect substitution was studied.Materials and methods. Prototyping of 3D constructors was performed by extrusion 3D printing. Various concentrations of vancomycin were added as an additional component at the stage of preparation of hydrogel (“ink”) for printing. Physical testing of the constructors included electron microscopic evaluation of their microstructure and porosity as well as the study of mechanical strength for compression and stretching. Functional activity of printed constructors with respect to the test strain S. aureus ATCC 6538-P was assessed by a disc-diffusion method in vitro and on the model of infected excision skin wound in rats -in vivo.Results. Analysis of the kinetic curves showed that the principal release of the drug in the model liquid took place within the first day. The total volume of the bound and released vancomycin made up approximately 20% of the calculated initial amount for all three concentrations. Formation of the marked growth inhibition zone of S. aureus strain in presence of vancomycin rich constructors was demonstrated in vitro. Insertion of constructors into the zone of infected skin defect resulted in a decrease of inflammatory processes severity and rate as well as accelerated the terms of complete wound healing.Conclusion. Study results showed the principle potentiality of bone substituting implants 3D-printing using multicomponent hydrogel compositions without change of separate components characteristics.

scholarly journals Calcium phosphate and composite materials functionalization of bioactive agents for its target delivery to the bone

2020 ◽  
Vol 27 (3) ◽  
pp. 52-59
Author(s):  
Ekaterina A. Kuvshinova ◽  
Natalia V. Petrakova ◽  
Natalya S. Sergeeva ◽  
Irina K. Sviridova ◽  
Valentina A. Kirsanova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Factors ◽  
Polymer Composite ◽  
Calcium Phosphates ◽  
Bioactive Molecules ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Growth Delay ◽  
Target Delivery

Aim of the study. The development of the method of octacalcium phosphate (OCP) and mineral-polymer composite material functionalization with biological agents (human platelet lysate (PL) growth factors and antibiotic vancomycin) by the biomimetic coprecipitation principle technique. Materials and methods. The OCP and the mineral-polymer composite matrices (sodium alginate / gelatin / OCP) functionalization was obtained by biomimetic coprecipitation of calcium phosphates and the bioactive molecules on their surface. The materials structure was examined by electron microscopy. The functionalization efficiency was determined by measurement of the incorporated compounds in solution, as well as by analysis of their release over the 8 days. The antimicrobial activity of vancomycin functionalized samples was evaluated by in vitro disk diffusion method against the Staphylococcus aureus strain. Results. The evaluation of incorporated molecules release showed that the OCP functionalization with vancomycin is more effective than PL. The antibiotic release had continued for three days, while PL growth factors only for 30 minutes. The incorporated into a composite matrix vancomycin was completely released within 24 h. In vitro study of the functionalized composite samples showed growth delay of the Staphylococcus aureus strain in dependence on antibiotic content. Conclusion. The developed method of drug incorporation during biomimetic precipitation allowed to create target delivery system which transfer antibiotic to the bone defect.

scholarly journals In-vitro Antibacterial Activity of Novel Nanostructured Composites Based on Forsterite and Silver Nanoparticles

2020 ◽  
Vol 71 (1) ◽  
pp. 13-21
Author(s):  
Alexandra Avram ◽  
Maria Gorea ◽  
Sorin Rapuntean ◽  
Aurora Mocanu ◽  
Gertrud Alexandra Paltinean ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Sol Gel ◽  
Trisodium Citrate ◽  
Inhibitory Effect ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Zones Of Inhibition

There is a continuous need for discovering new nanomaterials with antibacterial activity against various pathogens, like Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This study was performed to assess the antimicrobial activity of two novel nanostructured forsterites, both in the absence and the presence of silver nanoparticles (AgNPs). The two nano forsterites (FS) were prepared by advanced sol-gel (FSsg) and precipitation (FSpp) methods. Preparation of colloidal AgNPs systems was realized by using the precursor, AgNO3, and the trisodium citrate and tanic acid assuring the formation and stabilization of AgNPs. The characterization of nano forsterite powders was carried out using complementary physical methods: XRD, SEM, and AFM. The AgNPs were characterized by UV-Vis spectra, STEM and AFM imaging. The antimicrobial activity was studied by the agar well diffusion method both in the FS native state, as FSsg and FSpp, and in their mixture with silver nanoparticles (AgNPs). The inhibitory effect of synthesized forsterites, FSsg and FSpp, particularly variants with AgNPs was found only on the S. aureus strain, the zones of inhibition being between 8 and 10 mm, and more intensely expressed in the FSpp-AgNPs dispersions. These findings open new orthopedic applications of these systems, particularly for antimicrobial coated metallic implants.

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