scholarly journals In-vitro Antibacterial Activity of Novel Nanostructured Composites Based on Forsterite and Silver Nanoparticles

2020 ◽  
Vol 71 (1) ◽  
pp. 13-21
Author(s):  
Alexandra Avram ◽  
Maria Gorea ◽  
Sorin Rapuntean ◽  
Aurora Mocanu ◽  
Gertrud Alexandra Paltinean ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Sol Gel ◽  
Trisodium Citrate ◽  
Inhibitory Effect ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Zones Of Inhibition

There is a continuous need for discovering new nanomaterials with antibacterial activity against various pathogens, like Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This study was performed to assess the antimicrobial activity of two novel nanostructured forsterites, both in the absence and the presence of silver nanoparticles (AgNPs). The two nano forsterites (FS) were prepared by advanced sol-gel (FSsg) and precipitation (FSpp) methods. Preparation of colloidal AgNPs systems was realized by using the precursor, AgNO3, and the trisodium citrate and tanic acid assuring the formation and stabilization of AgNPs. The characterization of nano forsterite powders was carried out using complementary physical methods: XRD, SEM, and AFM. The AgNPs were characterized by UV-Vis spectra, STEM and AFM imaging. The antimicrobial activity was studied by the agar well diffusion method both in the FS native state, as FSsg and FSpp, and in their mixture with silver nanoparticles (AgNPs). The inhibitory effect of synthesized forsterites, FSsg and FSpp, particularly variants with AgNPs was found only on the S. aureus strain, the zones of inhibition being between 8 and 10 mm, and more intensely expressed in the FSpp-AgNPs dispersions. These findings open new orthopedic applications of these systems, particularly for antimicrobial coated metallic implants.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Helal F. Hetta ◽  
Israa M. S. Al-Kadmy ◽  
Saba Saadoon Khazaal ◽  
Suhad Abbas ◽  
Ahmed Suhail ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Microtiter Plate ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Infection Model ◽  
The Impact

AbstractWe aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

scholarly journals A new group of compounds derived from 4-, 5-, 6- and 7-aminoindoles with antimicrobial activity

2018 ◽  
Vol 4 (3) ◽  
pp. 27-36 ◽  
Author(s):  
Irina Stepanenko ◽  
Semen Yamashkin ◽  
Yuliya Kostina ◽  
Alyona Batarsheva ◽  
Mikhail Mironov
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Biologically Active ◽  
Diffusion Method ◽  
Significant Activity ◽  
Clinical Strains ◽  
Causative Agents ◽  
New Compounds

Introduction. The problem of antibiotic resistance of microorganisms is becoming more urgent in the twenty-first century. Microorganisms possess an evolutionary adaptive capacity. Non-adherence to the basic principles of rational antibiotic therapy leads to menacing consequences. More and more pathogenic microbes are becoming resistant to two or more antibiotics. The search for new compounds with antimicrobial activity is one of the principles for overcoming the antibiotic resistance of microorganisms. Materials and methods. Eighteen test-strains of microorganisms and more than 2000 clinical strains of microorganisms, representating the families Micrococcaceae, Streptococcaceae, Enterobacteriaceae, Moraxellaceae, Pseudomonadaceae, Sphingomonadaceae, Xanthomonadaceae were studied for sensitivity to the compounds derived from 4-, 5-, 6- and 7-aminoindoles. A method of serial dilutions to determine the minimal inhibitory concentration (MIC) of the compounds under study was used in the study, as well as a disc diffusion method. Results and discussion. Sensitivity of the test-strains and of clinical strains of microorganisms to the resulting compounds was studied. The compounds based on substituted 4-, 5-, 6-, 7-aminoindoles showed different activity against the test strains and experimental strains of microorganisms in vitro. It was found that the marked antibacterial activity was exhibited by the compounds containing a trifluoromethyl group. The most significant activity was noted in amides and pyrroloquinolones based on 4-aminoindole, 6-aminoindole and 7-aminoindole.The most effective compounds with laboratory codes 5D, 7D, 39D, S3, HD, 4D showed a pronounced antibacterial activity. Conclusion. Antimicrobial activity of the substituted amides and pyrroloquinolines on the basis of 4-, 5-, 6-, 7-aminoindoles was etermined in our study, as well as the spectra of their action against Gram-positive and Gram-negative microorganisms, which are causative agents of non-specific and certain specific human infectious diseases. Moreover, we evaluated the synthetic potentials of the substituted 4-, 5-, 6-, 7-aminoindoles as the starting compounds for synthesizing a series of indolylamides and pyrroloquinolines. Also, the prospects for targeted synthesis of biologically active compounds based on indole-type aromatic amines were determined.

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

2020 ◽  
Vol 2 (29) ◽  
pp. 32-36
Author(s):  
Semwal Amit Negi Sweta
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Disk Diffusion Method ◽  
Zanthoxylum Armatum ◽  
Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

scholarly journals Antibacterial ativity of biosynthesized silver nanoparticles against Pseudomonas aeruginosa in vitro

2017 ◽  
Vol 41 (1) ◽  
pp. 60-65
Author(s):  
Ahmad N. A. Salih ◽  
Mohammad J. Eesa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Sulfadiazine ◽  
Diffusion Method ◽  
Olive Leaves ◽  
Scanning Electron

     This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.                                                        

scholarly journals Potential Antibacterial Activity of Two Important Local Chewing Sticks “Fagara zanthoxyloides and Distemonanthus benthamianus” along with Antioxidant Capacities

2019 ◽  
Vol 18 (2) ◽  
pp. 223-232
Author(s):  
AO Shittu ◽  
A Aliyu ◽  
MS David ◽  
NS Njinga ◽  
HI Ishaq
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Zones Of Inhibition ◽  
Chewing Sticks

In order to authenticate and ascertain the various claim by the rural dwellers that depend on chewing sticks for their oral hygiene and protection against innumerable diseases, the antibacterial activity of the roots of Fagara zanthoxyloides and Distemonanthus benthamianus were evaluated. Preliminary phytochemical screening was carried out using standard methods. In-vitro antibacterial activity of the methanol extracts of both plants was also carried out using the agar well diffusion method against standard strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090 and also some clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis at different concentrations and ratios. Preliminary phytochemical screening showed that the extracts contained alkaloids, saponin, tannins, steroids, flavonoids, anthraquinones, cardiac glycosides and phenol. All the test microorganisms were susceptible to the inhibitory effect of the extracts at concentrations of 200 mg/ml and 500 mg/ml. F. zanthoxyloides had better antimicrobial activity with zones of inhibition ranging from 21.0 to 26.0 mm at 200 mg/ml and 24.3 to 29.3 mm at 500 mg/ml while D. benthamianus had zones of inhibition ranging from 17.7 to 26.7 mm at 200 mg/mL and 19.0 to 26.7 mm at 500 mg/mL. Some of the test microorganisms were resistant to the standard antibiotics (cefuroxime, ciprofloxacin, ofloxacin and gentamicin). Combinations of the extracts in ratios 50:50 and 75:25 yielded no increase in activity. The MIC and MBC for D. benthamianus ranged from 6.25 mg/ml to 100 mg/mL and 25 to 200 mg/ml, respectively while that of F. zanthoxyloides ranged from 1.56 mg/ml to 12.5 mg/mL and 50 to 200 mg/ml, respectively. The antimicrobial activity demonstrated by F. zanthoxyloides and D. benthamianus indicates that they would be valuable in the management of urinary, respiratory and gastrointestinal tracts infections while at the same time helping to mitigate the problem of antimicrobial resistance. Phenolic compounds of plants, among them flavonoids are the chief constituents, which have potent antioxidant activities Dhaka Univ. J. Pharm. Sci. 18(2): 223-232, 2019 (December)

scholarly journals In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms

2002 ◽  
Vol 13 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Caio Cezar Randi Ferraz ◽  
Morgana Eli Vianna ◽  
Pedro Luiz Rosalen ◽  
Alexandre Augusto Zaia ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Stainless Steel ◽  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Agar Medium ◽  
Diffusion Method ◽  
Root Canals ◽  
Zones Of Inhibition ◽  
Agar Diffusion Method

The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide in combination with several vehicles against some microorganisms commonly isolated from root canals. Antimicrobial activity was determined by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medicaments and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after incubation for each plate and the results were analyzed statistically (ANOVA). The in vitro antimicrobial effects of the medications were ranked from strongest to weakest as follows: Ca(OH)2 + CMCP + glycerine, Ca(OH)2 + CMCP, Ca(OH)2 + glycerine, Ca(OH)2 + anesthetic, Ca(OH)2 + saline, Ca(OH)2 + H2O, Ca(OH)2 + polyethyleneglycol. The pastes with oily vehicles showed significantly larger mean zones of inhibition compared to those with aqueous or viscous vehicles. It was concluded that diffusion and antimicrobial activity of calcium hydroxide were affected by the type of vehicle used.

scholarly journals In vitro antibacterial activity of Terminalia chebula leaf gall extracts against some human pathogenic strains

2012 ◽  
Vol 1 (8) ◽  
pp. 217-220 ◽  
Author(s):  
BE Ravi Shankara ◽  
YL Ramachandra ◽  
S Sundara Rajan ◽  
J Preetham ◽  
PS Sujan Ganapathy
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Terminalia Chebula ◽  
Bacterial Strains ◽  
South Indian

The leaf galls of Terminalia chebula is used widely as Karkatasringi in south Indian markets. Karkatashringi is an important crude drug employed in various indigenous systems of medicine against several diseases and the drug has diverse medicinal properties. The present study was carried out to understand the antimicrobial activity of various extracts. The antibacterial activity of T. chebula (leaf gall) was evaluated against ten bacterial strains including Gram-positive and Gram-negative bacteria using the agar-well diffusion method. Among the two extracts tested, the ethanol extract presented the best results against all the bacteria while aqueous extract showed moderate inhibition of the microbial growth. Each extract is unique against different microorganisms; Staphylococcus aureus was more susceptible to both extract among the tested organisms, whereas Serratia marcescens and Proteus mirabilis were less susceptible for ethanol and aqueous extract respectively. The inhibitory effect of the extracts was compared with standard antibiotic Ciprofloxacin.DOI: http://dx.doi.org/10.3329/icpj.v1i8.11254 International Current Pharmaceutical Journal 2012, 1(8): 217-220 

scholarly journals Comparison of Antimicrobial Activity of Child Formula Dentifrices at different Concentrations: An in vitro Study

2017 ◽  
Vol 10 (2) ◽  
pp. 131-135
Author(s):  
Ritika Malhotra ◽  
ND Shashikiran
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Fluoride Concentration ◽  
Agar Plate ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Distilled Water ◽  
Zones Of Inhibition

ABSTRACT Aim The aim of the present in vitro study is to evaluate and compare antimicrobial efficacy of commercially available child's dental formulas in reduced concentrations containing different forms of fluoride against Streptococcus mutans activity. Materials and methods The selected dentifrices were prepared in dilutions of 1:1, 1:2, 1:4, 1:8, and 1:16 using sterile pyrogen-free distilled water. Various dilutions of the selected toothpaste slurries were incubated in the agar plate containing pure strains of S. mutans, and antimicrobial activity of each was assessed by measuring the diameter of zones of inhibition (in mm). Agar well plate diffusion method and minimum inhibitory concentration (MIC) determination were the methods used in this study. The inhibitory circle of each dentifrice was measured and MIC was achieved by considering the value of diameter of the circle. Results The results of the study showed that even at a lower concentration of fluoride, inhibition halos were obtained for all the formulations at different dilutions. Conclusion The kid's formulations having lower fluoride concentration show antimicrobial activity even after dilutions. Thus, commercially, the fluoride concentrations can be further lowered down in the dentifrices, thereby reducing the risk associated with fluoride. How to cite this article Malhotra R, Singla S, Shashikiran ND. Comparison of Antimicrobial Activity of Child Formula Dentifrices at different Concentrations: An in vitro Study. Int J Clin Pediatr Dent 2017;10(2):131-135.

scholarly journals In vitro Antibacterial Activities of Honey Bee Extracts against Bacterial Isolates of Wound Infections

2021 ◽  
pp. 20-25
Author(s):  
O. A. Ayodele ◽  
J. O. Aribisala ◽  
A. T. Oseni ◽  
M. K. Oladunmoye
Keyword(s):  
Antibacterial Activity ◽  
Honey Bee ◽  
Honey Bees ◽  
Inhibitory Activity ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Wound Infections ◽  
Zones Of Inhibition

Microorganisms most especially bacteria, continue to develop resistance against antimicrobial agents; hence novel sources of antibiotics are urgently needed to reduce this problem. This study was carried out to investigate the antibacterial activities of ethanolic, chloroform and aqueous extracts of Apis mellifera (honey bee) on isolates of wound infections. The isolates used in this study were procured from University of Ilorin Teaching Hospital (UITH) and confirmed using morphological and biochemical tests. The isolates used include; Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae, Proteus mirabilis and Proteus vulgaris. Honey bees were collected from an apitherapist at Sunshine honey and agro foods, Akure, Ondo State Nigeria. The whole insect was used for in vitro antibacterial evaluation of the isolates using agar well diffusion method. Ethanolic extract of A. mellifera had the highest inhibitory activity with mean zones of inhibition ranging from 7.40 mm to 21.67 mm, chloroform extracts had moderate inhibitory activity ranging from 4.63 mm to 10.03 mm while the aqueous extract had the least activity with zones of inhibition ranging from 3.00 mm to 6.30 mm. However, no antibacterial activity was observed against P. aeruginosa for all the extracts. It is concluded that extracts of honey bees most especially the ethanolic extract have antibacterial activity and thus could be a potential antibacterial agent against isolates of wound infections.

Sign in / Sign up

Export Citation Format

Share Document