scholarly journals Pengaruh Cara Kematian dan Tahap Penurunan Mutu Filet Ikan Nila Merah (Oreochromis niloticus)

2021 ◽  
Vol 24 (2) ◽  
pp. 284-291
Author(s):  
Simson Masengi ◽  
Winda Sary ◽  
Yuliati Hotmauli Sipahutar
Keyword(s):  
Oreochromis Niloticus ◽  
Rigor Mortis

Perubahan yang dialami ikan setelah mati berlangsung dalam tiga fase, yaitu fase pre rigor, rigor mortis, dan post rigor. Tujuan dari penelitian ini adalah mengetahui pengaruh cara kematian dan waktu preparasi terhadap penurunan kesegaran ikan (pre rigor, rigor mortis, dan post rigor) yang disimpan pada suhu chilling. Pengolahan data dilakukan dengan ANOVA menggunakan rancangan acak kelompok (RAK) yang terdiri dari cara mati (menggelepar, di es, dan ditusuk segera) serta waktu preparasi dimulai (pre rigor, rigor dan post rigor) yang disimpan pada suhu chilling. Hasil penelitian pendahuluan berdasarkan parameter indeks rigor maupun nilai pH dari perlakuan ikan yang ditusuk mengalami penurunan mutu lebih lambat. Hasil penelitian utama laju kemunduran mutu filet ikan nila merah menunjukkan pengaruh nyata antara lama waktu penyimpanan pada setiap parameter uji filet ikan nila merah semua perlakuan. Berdasarkan parameter TVB dan nilai pH filet ikan nila merah mengalami laju penurunan mutu yang lebih lambat pada perlakuan dimatikan ditusuk dalam kondisi pre rigor.

INFLUÊNCIA DO ESTRESSE SOCIAL SOBRE O ESTABELECIMENTO DO RIGOR MORTIS EM TILÁPIAS DO NILO (Oreochromis niloticus)

2015 ◽  
Vol 3 (5) ◽  
pp. 61-73
Author(s):  
Sandro Alves CORRÊA
Keyword(s):  
Oreochromis Niloticus ◽  
Rigor Mortis

O objetivo deste estudo foi verificar a influência do estresse social sobre o estabelecimento do rigor mortis em tilápias do Nilo (Oreochromis niloticus) dominantes e submissas. Os animais experimentais (n=21) foram adaptados em aquários individuais durante sete dias. Após esse período, 14 tilápias foram submetidas ao pareamento (sete pares) por um período de seis horas e outras sete foram mantidas em isolamento (controle). Ao término dos pareamentos, os peixes foram identificados segundo sua posição hierárquica e submetidos à  coleta de sangue destinada à quantificação dos ní­veis plasmáticos de cortisol, glicose e proteínas totais. Após a coleta, os animais foram sacrificados e submetidos à análise do índice de rigor mortis (IRM). Os ní­veis plasmáticos de cortisol, glicose e proteí­nas totais não diferiram entre animais dominantes e submissos. Em comparação aos animais controle observou-se um aumento nos ní­veis plasmáticos de cortisol nos animais pareados (dominantes e submissos) (P<0,05) e nos níveis de glicose nos animais submissos (P<0,05). O IRM foi maior nos animais pareados, principalmente, submissos, comparados ao controle, porém, não diferiu entre animais de diferentes posições hierárquicas. Conclui-se que o estresse social reduz o tempo de vida útil do pescado, principalmente, em animais submissos. 

scholarly journals Efecto de la temperatura de almacenamiento sobre el rigor mortis del músculo de tilapia (Oreochromis niloticus)//Effect of storage temperature on tilapia muscle (Oreochromis niloticus) rigor mortis

Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 88-93
Author(s):  
Nathaly Montoya-Camacho ◽  
Enrique Márquez-Ríos ◽  
Francisco Javier Castillo-Yáñez ◽  
Saúl Ruíz-Cruz ◽  
José Luis Cárdenas-López ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Oreochromis Niloticus ◽  
Storage Time ◽  
Storage Temperature ◽  
Rigor Mortis ◽  
Acid Ph ◽  
Two Temperatures ◽  
Ir Analysis ◽  
And Storage

El objetivo de la presente investigación fue evaluar el efecto de la temperatura de almacenamiento (0 y 5ºC) sobre los principales metabolitos y parámetros relacionados con el rigor mortis del músculo de tilapia (Oreochromis niloticus). Se evaluó el índice de rigor (IR), adenosina 5´-trifosfato (ATP), glucógeno, ácido láctico, pH y textura. El análisis del IR se realizó durante 266 h de almacenamiento a las dos temperaturas, mientras que el resto de los parámetros se monitorearon al tiempo 0 y a las 48 h de almacenamiento en ambas temperaturas. Se encontró que tanto la temperatura como el tiempo de almacenamiento presentaron un efecto significativo sobre el IR, siendo más pronunciado a 5°C en comparación al de 0°C.ABSTRACTThe aim of this research was to evaluate the effect of storage temperature (0 and 5°C) on the main metabolites and related parameters to rigor mortis in muscle of tilapia (Oreochromis niloticus). The rigor index (IR), adenosine 5’-triphosphate (ATP), glycogen, lactic acid, pH and texture were evaluated. The IR analysis was performed during 266 hours of storage at the two temperatures, while the rest of the parameters were monitored at 0 and at 48 hours of storage at both temperatures. It was found that both temperature and storage time, had a significant effect on IR, being more pronounced at 5°C compared to 0°C.

scholarly journals Changes on the Development of Rigor Mortis in Cultured Tilapia (Oreochromis niloticus) Fed with a Mixture of Plant Proteins

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Nathaly Montoya Camacho ◽  
Enrique Márquez Ríos ◽  
Francisco Javier Castillo Yáñez ◽  
Saúl Ruíz Cruz ◽  
Aldo Alejandro Arvizu Flores ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Oreochromis Niloticus ◽  
Shear Force ◽  
Dietary Treatment ◽  
Average Weight ◽  
Farmed Fish ◽  
Rigor Mortis ◽  
Tank System ◽  
Main Protein

In recent years it has been pointed out that the feed of farmed fish has an effect on the quality of the final product. Therefore, this study evaluated the effect of fishmeal (FM) replacement by a mixture of plant protein (MPP) on the development of rigor mortis of tilapia (Oreochromis niloticus). One hundred and twenty fish at an initial average weight of 123 ± 6.3 g were fed with three extruded isonitrogenous and isolipidic 6.2% crude lipids experimental diets, in which FM were replaced by 0% (D0), 50% (D50), and 100% (D100) of MPP (soybean meal, corn meal, wheat meal, and sorghum meal). A reference diet (DC) containing FM as the main protein source was used as a control. The fish were divided into triplicate groups per dietary treatment. The experiment was conducted in a tank system at 26.8°C water temperature for 67 days. The chemical composition of experimental diets and muscle were determined. The glycogen, adenosine 5′-triphosphate (ATP) and related compounds, pH, shear force, and rigor index (RI%) were monitored during storage on ice for 48 h. The results indicated that FM replacement affected (p≤0.05) the muscle composition, where the fish fed with D100 presented the higher content of lipids and ash. Fish fed with D0 and DC presented a more pronounced onset of rigor mortis and also showed a higher IR%, a lower content of glycogen, ATP, adenosine 5′-diphosphate (ADP), adenosine 5′-monophosphate (AMP), pH, and shear force. The changes in chemical composition of muscle and other parameters evaluated indicated that FM replacement increases energy reserves (glycogen, ATP, ADP, and AMP) which delayed the onset of rigor mortis, as well as a lower pH and shear force in the muscle of tilapia. Therefore, the substitution of FM by MPP could contribute to delaying the onset of rigor mortis and with this, the quality and shelf life of tilapia could be increased.

Ultrastructure of Testicular Spermatozoon of the Fish Oreochromis Niloticus

1988 ◽  
Vol 46 ◽  
pp. 278-279
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Electron Microscope ◽  
Oreochromis Niloticus ◽  
Osmium Tetroxide ◽  
Sperm Cells ◽  
Adult Fish ◽  
Testicular Sperm ◽  
Thin Sections ◽  
Important Fish ◽  
Testicular Spermatozoon ◽  
Diamond Knife

Tilapia, Oreochromis niloticus, is an economically important fish in Saudi Arabia. Elucidation of reproductive biology of this species is necessary for successful breeding program. In this paper we describe fine structure of testicular sperm cells in O, niloticus.Testes from young adult fish were fixed in gluteraldehyde (2%) and osmium tetroxide (1%), both in cacodyl ate buffer. Specimens were processed in the conventional way for electron microscopy and thin sections of tissues (obtained by cutting the blocks with a diamond knife) were stained by ura- nyl acetate and lead citrate. These were examined in a Carl Zeiss electron microscope operated at 40 kV to 60 kV. Sperm cells were obtained from testes by squeezing them in cacodyl ate buffer. They were fixed in gluteraldehyde (2%) in the same buffer, air dried, gold coated and then examined in a Philips scanning electron microscope (SEM) operated at 25kV.The spermatozoon of O. niloticus is consisting of head, midpiece and tail (Fig. 1).

Ultrastructure of primary spermatocyte in fish (Tilapia: Oreochromis niloticus): The synaptonemal complex

1986 ◽  
Vol 44 ◽  
pp. 288-289
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Synaptonemal Complex ◽  
Oreochromis Niloticus ◽  
Short Communication ◽  
Primary Spermatocyte ◽  
Mitotic Division ◽  
Meiotic Cell ◽  
Electron Microscope Level ◽  
Homologous Chromosomes

The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we are reporting the Ultrastructure of Primary Spermatocytes in tilapia, O. niloticus, and the fine structure of synaptonemal complexes seen in the spermatocyte nuclei.

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