Isolation and Characterization of Bacterial Endophytes from Ramie (Boehmeria nivea)

Endophytic bacteria provide several advantages to the host plant by promoting growth as well as conferring resistance against pathogens and toxic compounds. More than 90% of the cultivation of ramie [Boehmeria nivea (L.) Gaud] is contributed by China. In our study, 265 isolates of bacterial endophytes were obtained from ramie plants. The isolates were then subjected to PCR-based screening. Four endophytic bacterial phyla were identified using 16S rDNA sequencing. The identified dominant microorganisms were Janibacter melonis, Moraxella, and Bacillus pumilus in the root, stem, and leaf, respectively. The results suggest that these endophytes isolated from the ramie have the potential to act as biocontrol agents against pathogens such as Sclerotinia, Colletotrichum, and Phytophthora. However, further study is required to examine the potential application of bacterial endophytes, particularly as biological control agents. © 2021 Friends Science Publishers

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Diversity of Endophtic Bacteria in Aerides crispa, an Epiphytic Orchid

International Journal of Scientific Research in Science and Technology ◽

10.32628/ijsrst21822 ◽

2021 ◽

pp. 07-16

Author(s):

Darsha S ◽

Jayashankar M

Keyword(s):

Endophytic Bacteria ◽

Bacillus Pumilus ◽

Bacterial Culture ◽

Pcr Amplification ◽

Epiphytic Orchid ◽

Tree Generation ◽

Rdna Sequencing ◽

The Family ◽

Relationship Of

Aerides crispa is an epiphytic flowering orchid belongs to the family Orchidaceae.we have identified five different endophytic bacteria from this orchid. Genotypic characterization of the bacterial culture was done using 16S rDNA sequencing after PCR amplification. These sequences were compared with the known similar bacterial sequences from the NCBI GenBank database. Homological relationship of the bacteria was also compared by Phylogenetic tree generation. Bacteria obtained were Bacillus pumilus MT463728, Bacillus megaterium MT540506, Lysinibacillus fusiformis MT540507, Bacillus cereus MT540510, Aneurinibacillus migulanus. From the study, we have molecularly characterized and documented five different endophytic bacteria from the epiphytic orchid, A crispa. This shows the diversity of endophytes in orchids which speaks a lot about the urgent necessity of bioconservation without words.

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Isolation and Characterization of Bacterial Endophytes from Lycopersicon esculentum Plant and their Plant Growth Promoting Characteristics

Nepal Journal of Biotechnology ◽

10.3126/njb.v2i1.5679 ◽

1970 ◽

Vol 2 (1) ◽

pp. 37-52 ◽

Cited By ~ 19

Author(s):

Hardik A Patel ◽

Rajesh K Patel ◽

Sunil M Khristi ◽

Kruti Parikh ◽

Geetha Rajendran

Keyword(s):

Lycopersicon Esculentum ◽

Plant Growth ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Bacterial Endophytes ◽

Isolation And Characterization ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Different Characteristics

The study was designed to isolate and characterize bacterial endophytes from root and stem of Lycopersicon esculentum plant which was collected form different region of Gujarat. Total 18 isolates of endophytic bacteria were selected in which, all the endophytic bacteria produced one or the other different characteristics involved in plant growth promotion. They either produced phytohormones like indole acetic acid, siderophore, protease, pectinase, organic acid showed antifungal activity, chromium tolerance and solubilized phosphate. Four of the strains among the 18 showed maximum positive results of plant growth promoting regulators (PGPR) test and among them best probable isolate was selected and thus its 16SrDNA was amplified and sequenced. Only HR7 endophyte of tomato turned out to be Pseudomonas aeruginosa. It’s a gram negative coccobacili, sporeforming motile bacilli and show maximum PGPR activity. The results of our present studies indicated that above strains might be endophytic and therefore, were associated with the plant growth. Keywords: Lycopersicon esculentum; endophytic bacteria; PGPR; IAA; 16SrDNA DOI: http://dx.doi.org/10.3126/njb.v2i1.5679 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 37-52

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