scholarly journals Techniques for Improving the Tissue Culture Efficiency of Purple Passion Fruit (Passiflora edulis)

2021 ◽  
Vol 25 (02) ◽  
pp. 469-474
Author(s):  
Dongmei Huang
Keyword(s):  
Tissue Culture ◽  
Southern China ◽  
Shoot Proliferation ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Chlorophyll Contents ◽  
Rooting Medium ◽  
Bud Induction ◽  
Purple Passion Fruit ◽  
Stem Segments

Purple passion fruit (Passiflora edulis Sims) has gained attention in Southern China, and its planting area has increased during the last several years. Through tissue culturing, virus-free plants are produced as maternal parents for seedling production. However, there are some difficulties that affect passion fruit tissue culture efficiency, including high contamination rates in explant disinfection, low shoot proliferation, yellow or albino leaves, slow growth, and time-consuming processes. In this work, the aforementioned problems were investigated, and disinfection was optimized. Results revealed that the repeat disinfection method (0.1% HgCl2 for 15 min + 0.1% HgCl2 for 12 min) with a 2-d interval was the most suitable disinfection treatment for young stem segments of purple passion fruit. The addition of silver thiosulfate (STS) improved proliferation efficiency. Moreover, additional 1X iron salt was added to the bud induction and rooting medium. The regenerated shoots had a better seedling state with healthier green leaves, roots were more easily induced and better developed and the chlorophyll contents were higher. Thus, more efficient tissue culturing of purple passion fruit was achieved. © 2021 Friends Science Publishers

scholarly journals Effect of NaCl salinity on seed germination and seedling emergence of purple passion fruit (Passiflora edulis Sims)

2014 ◽  
Vol 32 (2) ◽  
pp. 188-195 ◽  
Author(s):  
Luis Ariel Montaña ◽  
Gerhard Fischer ◽  
Stanislav Magnitskiy ◽  
Guillermo Zuluaga
Keyword(s):  
Saline Solution ◽  
Seedling Emergence ◽  
Petri Dish ◽  
Passion Fruit ◽  
Germination Test ◽  
River Sand ◽  
Transparent Plastic ◽  
Passiflora Edulis ◽  
Chlorophyll Contents ◽  
Purple Passion Fruit

The purple passion fruit is propagated by seeds, but factors, such as hardiness and impermeability of the testa and salinity and pH of the soil, give rise to problems in germination and uniformity of seedlings. The objectives of the study were to evaluate the effect of different NaCl concentrations (0, 30, 60, 90, and 120 mM, corresponding to 0.8, 3.0, 6.0, 9.0, and 12.2 dS m-1) on the germination and emergence of purple passion fruit seeds. For the germination test, 50 seeds per Petri dish were used, which were watered with a saline solution weekly. A seed was considered germinated when the radicle reached 2 mm. In the case of seedling emergence, 50 seeds were sown in cleaned river sand at a 1 cm depth on polystyrene trays, covered with transparent plastic film. They were irrigated weekly with different NaCl concentrations and the electrical conductivity (EC) of the substrate was measured. A seedling was considered emerged when the hypocotyl was fully erect. The results showed significant differences, with germination being higher in seeds treated with 30 mM NaCl than in the control seeds, and no statistical differences for the 60 and 90 mM NaCl treatments. The emergence was significantly higher in the 0 (0.05 dS m-1 of the substrate) and 30 mM NaCl (0.71 dS m-1) treated seeds when compared with 60 mM (1.25 dS m-1), 90 mM (1.69 dS m-1) and 120 mM NaCl (2.30 dS m-1 of the substrate). There was a decline in the chlorophyll contents of the seedling cotyledons and an increased substrate EC with increasing NaCl concentrations.

scholarly journals Review on the ecophysiology of important Andean fruits: Passiflora L.

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Gerhard Fischer ◽  
Diego Miranda
Keyword(s):  
Climatic Factors ◽  
Fruit Trees ◽  
Passion Fruit ◽  
Flower Bud ◽  
Flower Opening ◽  
Passiflora Edulis ◽  
Bud Induction ◽  
Strong Winds ◽  
Purple Passion Fruit ◽  
Yellow Passion Fruit

The development of Andean fruit crops is viewed as an important and healthy contribution to global food consumption but ecophysiological studies on these fruit trees are scarce. 96% of approximately 520 Passiflora L. species are distributed in the Americas, especially in Colombia and Brazil. Many of these species originated on the edges of humid forests in tropical valleys. The four species: yellow passion fruit (Passiflora edulis f. flavicarpa Degener), sweet granadilla (Passiflora ligularis Juss.), purple passion fruit (Passiflora edulis f. edulis Sims) and banana passion fruit (Passiflora tripartita var. mollissima (Kunth) Holm-Niels & P.M. Jørg) are widely cultivated in Colombia, and their ecophysiological findings are described in this review. The demands, in terms of temperature (°C) and altitude (masl) are, for yellow passion fruits: 15-28 °C and 0-1,300 masl; sweet granadillas: 15-23 °C and 1,800-2,600 masl; purple passion fruits: 15-22/12-14 °C (day/night) and 1,600-2,300 masl; and banana passion fruit: 13-16 °C and 1.800-3.200 masl; all of them have high requirements for solar radiation, a minimum of 7 h of sunshine per day, to encourage flowering and fruit quality. Cloudy days decrease growth, flower bud induction and flower opening. Temperature and photosynthetic active radiation are the climatic factors that have the greatest effect on plant development. Relative humidity between 60 and 80% supports effective pollination and fecundation. Passiflora L. crops do not support long periods of waterlogging, with a maximum of 4 days for yellow passion fruit. Climatic events such as prolonged rain, intense droughts, strong winds and hail are harmful for these plants.

scholarly journals Genetic Diversity of Purple Passion Fruit, Passiflora edulis f. edulis, Based on Single-Nucleotide Polymorphism Markers Discovered through Genotyping by Sequencing

Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 144
Author(s):  
Nohra Castillo Rodríguez ◽  
Xingbo Wu ◽  
María Isabel Chacón ◽  
Luz Marina Melgarejo ◽  
Matthew Wohlgemuth Blair
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphism ◽  
Genotyping By Sequencing ◽  
Passion Fruit ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Passiflora Edulis ◽  
Tropical Fruit ◽  
Commercial Cultivars ◽  
Purple Passion Fruit

Orphan crops, which include many of the tropical fruit species used in the juice industry, lack genomic resources and breeding efforts. Typical of this dilemma is the lack of commercial cultivars of purple passion fruit, Passiflora edulis f. edulis, and of information on the genetic resources of its substantial semiwild gene pool. In this study, we develop single-nucleotide polymorphism (SNP) markers for the species and show that the genetic diversity of this fruit crop has been reduced because of selection for cultivated genotypes compared to the semiwild landraces in its center of diversity. A specific objective of the present study was to determine the genetic diversity of cultivars, genebank accession, and landraces through genotyping by sequencing (GBS) and to conduct molecular evaluation of a broad collection for the species P. edulis from a source country, Colombia. We included control genotypes of yellow passion fruit, P. edulis f. flavicarpa. The goal was to evaluate differences between fruit types and compare landraces and genebank accessions from in situ accessions collected from farmers. In total, 3820 SNPs were identified as informative for this diversity study. However, the majority distinguished yellow and purple passion fruit, with 966 SNPs useful in purple passion fruits alone. In the population structure analysis, purple passion fruits were very distinct from the yellow ones. The results for purple passion fruits alone showed reduced diversity for the commercial cultivars while highlighting the higher diversity found among landraces from wild or semi-wild conditions. These landraces had higher heterozygosity, polymorphism, and overall genetic diversity. The implications for genetics and breeding as well as evolution and ecology of purple passion fruits based on the extant landrace diversity are discussed with consideration of manual or pollinator-assisted hybridization of this species.

QUALITY CHANGES OF PURPLE PASSION FRUIT (PASSIFLORA EDULIS) DURING STORAGE

2008 ◽  
pp. 239-244 ◽  
Author(s):  
W.C. Schotsmans ◽  
S.E. Nicholson ◽  
S. Pinnamaneni ◽  
A.J. Mawson
Keyword(s):  
Passion Fruit ◽  
Quality Changes ◽  
Passiflora Edulis ◽  
Purple Passion Fruit

scholarly journals Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 231 ◽  
Author(s):  
Nohra C. Rodríguez ◽  
Luz M. Melgarejo ◽  
Matthew W. Blair
Keyword(s):  
Water Status ◽  
High Elevation ◽  
Passion Fruit ◽  
Physiological Adaptation ◽  
Apparent Quantum Yield ◽  
Important Conclusion ◽  
Passiflora Edulis ◽  
Chlorophylls A And B ◽  
Passiflora Edulis Sims ◽  
Purple Passion Fruit

Purple passion fruit (Passiflora edulis Sims f. edulis) is a tropical juice source. The goal of this project was to evaluate photosynthetic and physiological variability for the crop with the hypotheses that landraces contain the diversity to adapt to higher elevation nontraditional growing environments and this is dependent on specific parameters of ecological adaptation. A total of 50 genotypes of this crop were chosen from divergent sources for evaluations of their eco-physiological responses in two equatorial locations at different altitudes in the Andes Mountains, a center of diversity for the species. The germplasm included 34 landraces, 8 commercial cultivars, and 8 genebank accessions. The two locations were contrasting in climates, representing mid and high elevations in Colombia. Mid-elevation valleys are typical regions of production for passion fruit while high elevation sites are not traditional. The location effects and variables that differentiated genotypes were determined. Results showed statistically significant differences between locations and importance of physiological parameters related to photosynthesis and water use efficiency. Some landraces exhibited better water status and gas exchange than commercial types. Parameters like maximum photosynthesis, points of light saturation and compensation, darkness respiratory rate, and apparent quantum yield varied between genotype groups. The landraces, commercial types, and genebank entries also differed in content of carotenoids and chlorophylls a and b. Meanwhile, photosynthesis measurements showed that altitudinal difference had an effect on genotype-specific plant growth and adaptation. An important conclusion was that landraces contained the diversity to adapt to the new growing environment at higher altitudes.

scholarly journals Identification and pathogenicity of microorganisms affecting purple passion fruit in Colombia

Revista CERES ◽  
2017 ◽  
Vol 64 (3) ◽  
pp. 250-257
Author(s):  
Joaquín Guillermo Ramírez Gil ◽  
Pablo Julián Tamayo ◽  
Juan Gonzalo Morales
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Passion Fruit ◽  
Phytophthora Nicotianae ◽  
Passiflora Edulis ◽  
Fruit Samples ◽  
Phytophthora Drechsleri ◽  
Pathogenicity Tests ◽  
Purple Passion Fruit ◽  
First Time

ABSTRACT Purple passion fruit (Passiflora edulis f. edulis Sims, known in Colombia as “gulupa”) is an increasingly important crop in Colombia, as seen by an increase in the area under cultivation. This recent prominence coincides with a low number of existing technologies related to the cultivation of the fruit, resulting in a lack of knowledge about its associated pathogens and the absence of rapid and precise diagnostic tests. The objective of this study was to determine the pathogenicity of distinct microorganisms isolated from purple passion fruit samples, some of which had not been previously reported in Colombia. The sampling was performed in seven plots located in two regions of the Colombian Antioquia. With the use of field symptomatology, the isolation of microorganisms, morphological characteristics, molecular analysis, and pathogenicity tests, the pathogen species Phytophthora nicotianae var. parasitica, Colletotrichum gloeosporioides sensu lato, and Phytophthora drechsleri were identified for the first time in Colombia. These pathogens cause lesions in fruits, with an incidence of 10.9, 7.6, and 2.8%, respectively. This is also the first report on a global level of Phytophthora drechsleri causing a disease in this plant species.

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