scholarly journals Association of ACE DD Genotype with Hypertension among the Tribal Populations of South India

2016 ◽  
Vol 52 ◽  
pp. 1-8 ◽  
Author(s):  
Raghu Paramasivam ◽  
Nandhakumar Rengasamy ◽  
Deva Arumugam ◽  
Prabhakaran Krishnan
Keyword(s):  
South India ◽  
Renin Angiotensin System ◽  
Chain Reaction ◽  
Specific Primers ◽  
Angiotensin System ◽  
Allele Specific ◽  
Tribal Populations ◽  
Important Regulator ◽  
Polymerase Chain ◽  
Vasoactive Peptide

The Renin-Angiotensin System (RAS) is an important regulator of the blood pressure (BP). The level of the vasoactive peptide Angiotensin-II, is mainly determined by the RAS enzyme, angiotensin converting enzyme-1 (ACE-1). Polymorphisms in ACE gene is reported to be associated with hypertension in various populations worldwide. We investigated the association of ACE I/D polymorphisms with hypertension among the tribal populations of South India. Samples were collected from hypertensive patients (n = 33) and healthy controls (n = 37). Genotyping was performed using Polymerase chain reaction (PCR) with allele specific primers. The DD genotype is significantly observed among the cases (OR = 1.0). Specifically, the DD genotype is more evident among the females (OR = 0 .705) than males (OR = 1.22) and is analysed to be associated with hypertension among the tribal populations of South India.

scholarly journals Irbesartan prevents sodium channel remodeling in a canine model of atrial fibrillation

2018 ◽  
Vol 19 (1) ◽  
pp. 147032031875526 ◽  
Author(s):  
Xuewen Wang ◽  
Guangping Li
Keyword(s):  
Atrial Fibrillation ◽  
Sodium Current ◽  
Renin Angiotensin System ◽  
Canine Model ◽  
Atrial Pacing ◽  
Chain Reaction ◽  
Patch Clamp Technique ◽  
Angiotensin System ◽  
Whole Cell Patch Clamp ◽  
Polymerase Chain

Introduction: Activation of the renin-angiotensin system (RAS) plays an important role in atrial electrical remodeling (AER). The purpose of the present study was to evaluate the effects of irbesartan on cardiac sodium current (INa) in a canine model of atrial fibrillation. Materials and methods: Eighteen dogs were randomized into sham, pacing or pacing+irbesartan groups ( n = 6 in each group). The dogs in the pacing and irbesartan group were paced at 500 bpm for two weeks. Irbesartan (60 mg·kg−1·d−1) was administered orally in the pacing+irbesartan groups. INa was recorded using the whole-cell patch clamp technique from canine atrial myocytes. The expressions of cardiac Na+ channels (Nav1.5) mRNA were semi-quantified by reverse transcription-polymerase chain reaction. Results: Our results showed that INa density and Nav1.5 mRNA expression in the pacing group decreased significantly ( p < 0.05 vs. sham). However, rapid atrial pacing had no effects on the half-activation voltage (V1/2act) and half-inactivation voltage (V1/2inact) of INa ( p > 0.05 vs. sham). Irbesartan significantly increased INa densities and gene expression and hyperpolarized V1/2act without concomitant changes in V1/2inact. Conclusions: Irbesartan significantly increased INa densities, which contributed to improving intra-atrial conduction and prevented the induction and promotion of AF in atrial pacing dogs.

Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers

Transfusion ◽  
1994 ◽  
Vol 34 (11) ◽  
pp. 955-960 ◽  
Author(s):  
B Skogen ◽  
DB Bellissimo ◽  
MJ Hessner ◽  
S Santoso ◽  
RH Aster ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Determination ◽  
Chain Reaction ◽  
Specific Primers ◽  
Allele Specific ◽  
Polymerase Chain

The Polymerase Chain Reaction with Sequence Specific Primers for the Detection of the Factor V Mutation Associated with Activated Protein C Resistance

1995 ◽  
Vol 74 (03) ◽  
pp. 874-878 ◽  
Author(s):  
Nancy E Kirschbaum ◽  
Paul A Foster
Keyword(s):  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Factor V ◽  
Activated Protein C Resistance ◽  
Chain Reaction ◽  
Specific Primers ◽  
Allele Specific ◽  
Fv Leiden ◽  
Polymerase Chain

SummaryThe prevalence of the Factor V (FV) mutation associated with activated protein C resistance (FV Leiden) and its significance as a genetic risk factor for venous thrombosis have necessitated the development of a simple, rapid, and accurate assay for its detection. The polymerase chain reaction with sequence specific primers (PCR-SSP) provides a powerful technique for the discrimination of alleles resulting from single base substitutions. PCR amplification was performed using a sense primer complementary to both FV alleles coupled with either of two antisense allele specific primers, one complementary to the normal FV allele and one complementary to the FV Leiden allele. PCR conditions were developed that favored amplification only in the case of perfect complementation between template DNA and allele specific primer. The FV genotype was assigned based on whether or not each allele specific primer set produced an amplified product. Assignment of genotypes correlated 100% with those determined by the method of PCR amplification followed by MnII digestion. PCR-SSP allows the rapid and accurate identification of carriers of the Factor V Leiden mutation by a simple PCR reaction without the need for the usual post-amplification specificity step.

scholarly journals Duffy blood group genotypes among malaria patients in Rondônia, Western Brazilian Amazon

2001 ◽  
Vol 34 (6) ◽  
pp. 591-595 ◽  
Author(s):  
Carlos Eugênio Cavasini ◽  
Fabrício José Tarelho Pereira ◽  
Weber Luidi Ribeiro ◽  
Gerhard Wunderlich ◽  
Marcelo Urbano Ferreira
Keyword(s):  
Blood Group ◽  
Antigen Expression ◽  
Brazilian Amazon ◽  
Genotype Distribution ◽  
Chain Reaction ◽  
Specific Primers ◽  
Duffy Blood Group ◽  
Duffy Antigen ◽  
Allele Specific ◽  
Polymerase Chain

We have compared Duffy blood group genotype distribution, as determined by polymerase chain reaction with allele-specific primers, in 68 Plasmodium vivax-infected patients and 59 non-vivax malaria controls from Rondônia, Brazil. Homozygosity for the allele Fy, which abolishes Duffy antigen expression on erythrocytes, was observed in 12% non-vivax controls but in no P. vivax patient. However, no significant association was found between Fy heterozygosity and protection against P. vivax. The Fy x allele, which has recently been associated with very weak erythrocyte expression of Duffy antigen, was not found in local P. vivax patients.

scholarly journals Molecular selection of Beta vulgaris L. breeding materials with genes of resistance to abiotic stresses

2019 ◽  
Vol 1 (1) ◽  
pp. 16-20
Author(s):  
A. A. Nalbandyan ◽  
T. P. Fedulova ◽  
A. S. Hussein
Keyword(s):  
Sugar Beet ◽  
Molecular Genetic ◽  
Polymerase Chain Reaction Method ◽  
Root Knot Nematode ◽  
Chain Reaction ◽  
Specific Primers ◽  
Allele Specific ◽  
Molecular Selection ◽  
Polymerase Chain ◽  
Selection Of

In the work, the results of sugar beet breeding materials' molecular-genetic studying for presence of genes of resistance to root-knot nematode, rhizomania and powdery mildew are presented. Testing of plants was conducted using polymerase chain reaction method. The genes R6m-1, Rz1 and Rz2, Pm were identified with the help of 5 one-chain RAPD and 4 allele-specific primers. Aim of the studies is to screen sugar beet varieties for presence of the abovementioned genes of resistance. Domestic and foreign sugar beet hybrids were an object of the studies.

Duffy blood group genotyping in French Basques using polymerase chain reaction with allele-specific primers (PCR-ASP)

2003 ◽  
Vol 16 (1) ◽  
pp. 78-81 ◽  
Author(s):  
Fr�d�ric Bauduer ◽  
Mhammed Touinssi ◽  
Anna Degioanni ◽  
St�phane Leroux ◽  
Olivier Dutour ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Group ◽  
Chain Reaction ◽  
Specific Primers ◽  
Duffy Blood Group ◽  
Allele Specific ◽  
Polymerase Chain
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