scholarly journals Diagnostic value of measurement of exhaled endogenous nonvolatile substances

2008 ◽  
pp. 86-90
Author(s):  
V. A. Dobrykh ◽  
I. E. Mun ◽  
O. P. Gnatjuk
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Renal Insufficiency ◽  
Chronic Renal Insufficiency ◽  
Exhaled Breath Condensate ◽  
Diagnostic Value ◽  
Renal Diseases ◽  
Exhaled Breath ◽  
Breath Condensate ◽  
Healthy Persons

The total amount of nonvolatile substances in exhaled breath condensate (EBC) is a biomarker of formation of dispersed aerosol in airways. Exhaled endogenous nonvolatile substances have been studied in 358 healthy persons and patients with respiratory, heart, and renal diseases. The exhalation of endogenous nonvolatile substances was not associated with gender but enhanced with aging. Smoking, acute period of pneumonia, and chronic renal insufficiency increased nonvolatile substances volume in EBC. Chronic heart failure and COPD reduced this parameter. Concentration of nonvolatile substances in EBC increased in all these diseases. Proposed diagnostic test could be useful for investigation of EBC.

Exhaled Breath Condensate in Acute and Chronic Heart Failure: New Insights into the Role of Lung Injury and Barrier Dysfunction

2014 ◽  
Vol 190 (3) ◽  
pp. 342-345 ◽  
Author(s):  
Loukas K. Pappas ◽  
Georgios Giannopoulos ◽  
Stelios Loukides ◽  
Gerasimos Gavrielatos ◽  
Efrossini Athanasopoulou ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Lung Injury ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Barrier Dysfunction ◽  
Breath Condensate

scholarly journals SIGNS OF INFLAMATION IN THE AIRWAYS IN CHILDREN WITH EXERCISE-INDUCED BRONCHIAL ASTHMA

2017 ◽  
Vol 4 (1) ◽  
pp. 14-19 ◽  
Author(s):  
M.N. Garas
Keyword(s):  
Key Words ◽  
Proteolytic Activity ◽  
Bronchial Asthma ◽  
Exhaled Breath Condensate ◽  
Nitrogen Monoxide ◽  
Diagnostic Value ◽  
Exhaled Breath ◽  
Asthma Phenotype ◽  
Exercise Induced ◽  
Breath Condensate

SIGNS OF INFLAMATION IN THE AIRWAYS IN CHILDREN WITH EXERCISE-INDUCED BRONCHIAL ASTHMAGaras M.N., Bilous T.M., Lekhkun G.V.In the article based on examination of 102 children with bronchial asthma was defined indices of inflammatory activity of the airways and was set the diagnostic value of these indices as tests for verification exercise-induced bronchial asthma. It was shown that the children with no signs of exercise-induced asthma compared to patients having them, experience some changes in the exhaled breath condensate, indicating a higher activity of inflammation in the airways. The content of nitrogen monoxide metabolites less than 50 mcmol/L or markers of proteolytic activity by azocol lysis less than 0,2 ml/hour in pulmonary expiration products increase the chances of having exercise-induced bronchial asthma phenotype with these tests sensitivity within 66,7-75,7%.Key words: exercise-induced bronchial asthma, children, diagnostics, inflammation of airways, pulmonary expiration. ПОКАЗНИКИ АКТИВНОСТІ ЗАПАЛЬНОГО ПРОЦЕСУ ДИХАЛЬНИХ ШЛЯХІВ У ДІТЕЙ, ХВОРИХ НА БРОНХІАЛЬНУ АСТМУ ФІЗИЧНОЇ НАПРУГИ Гарас М.Н., Білоус Т.М., Лехкун Г.В.У статті на підставі обстеження 102 дітей, хворих на бронхіальну астму, було визначено показники активності запального процесу дихальних шляхів, а також установлено діагностичну цінність даних показників як тестів по верифікації бронхіальної астми фізичної напруги. Установлено, що у дітей без ознак бронхіальної астми фізичної напруги порівняно з пацієнтами за їх наявності виявляються зміни у конденсаті видихуваного повітря, що свідчать про вищу активність запального процесу в дихальних шляхах. Уміст у легеневому експіраті метаболітів монооксиду нітрогену менше 50 мкмоль/л або маркерів протеолітичної активності за лізисом азоколу менше 0,2 мл/год у два рази підвищують шанси наявності фенотипу астми фізичної напруги при чутливості даних тестів у межах 66,7-75,7%.Ключові слова: бронхіальна астма фізичної напруги, діти, діагностика, запалення дихальних шляхів, легеневий експірат. ПОКАЗАТЕЛИ АКТИВНОСТИ ВОСПАЛИТЕЛЬНОГО ПРОЦЕССА ДЫХАТЕЛЬНЫХ ПУТЕЙ У ДЕТЕЙ, БОЛЬНЫХ БРОНХИАЛЬНОЙ АСТМОЙ ФИЗИЧЕСКОГО НАПРЯЖЕНИЯ.Гарас Н.Н., Билоус Т.М., Лехкун Г.В.В статье на основании обследования 102 детей, больных бронхиальной астмой, были определены показатели активности воспалительного процесса дыхательных путей, а также установлена диагностическую ценность данных показателей как тестов по верификации бронхиальной астмы физического напряжения. Показано, что у детей без признаков бронхиальной астмы физического напряжения по сравнению с пациентами при их наличии выявляются изменения в конденсате выдыхаемого воздуха, которые свидетельствуют о более высокой активности воспалительного процесса в дыхательных путях. Содержание в легочном экспиратов метаболитов окиси азота меньше 50 мкмоль/л или маркеров протеолитической активности по лизису азокола меньше 0,2 мл/ч в два раза повышают шансы наличии фенотипа астмы физического напряжения при чувствительности данных тестов в пределах 66,7-75,7%.Ключевые слова: бронхильная астма физического напряжения, воспаление дыхательных путей, дети, диагностика, легочный экспират.

Increased keratin content detected by proteomic analysis of exhaled breath condensate from healthy persons who smoke

2004 ◽  
Vol 117 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Elisabetta Gianazza ◽  
Luigi Allegra ◽  
Enrica Bucchioni ◽  
Ivano Eberini ◽  
Lina Puglisi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate ◽  
Healthy Persons

scholarly journals Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics

2017 ◽  
Vol 313 (4) ◽  
pp. L664-L676 ◽  
Author(s):  
Laura M. López-Sánchez ◽  
Bernabé Jurado-Gámez ◽  
Nuria Feu-Collado ◽  
Araceli Valverde ◽  
Amanda Cañas ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Proteomic Analysis ◽  
Exhaled Breath Condensate ◽  
Characteristic Curve ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Chronic Obstructive ◽  
Exhaled Breath ◽  
Diagnosis Of Lung Cancer ◽  
Breath Condensate

We explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide biomarkers for noninvasive screening for the early detection of lung cancer (LC). EBC was collected from 192 individuals [49 control (C), 49 risk factor-smoking (S), 46 chronic obstructive pulmonary disease (COPD) and 48 LC]. With the use of liquid chromatography and tandem mass spectrometry, 348 different proteins with a different pattern among the four groups were identified in EBC samples. Significantly more proteins were identified in the EBC from LC compared with other groups (C: 12.4 ± 1.3; S: 15.3 ± 1; COPD: 14 ± 1.6; LC: 24.2 ± 3.6; P = 0.0001). Furthermore, the average number of proteins identified per sample was significantly higher in LC patients, and receiver operating characteristic curve (ROC) analysis showed an area under the curve of 0.8, indicating diagnostic value. Proteins frequently detected in EBC, such as dermcidin and hornerin, along with others much less frequently detected, such as hemoglobin and histones, were identified. Cytokeratins (KRTs) were the most abundant proteins in EBC samples, and levels of KRT6A, KRT6B, and KRT6C isoforms were significantly higher in samples from LC patients ( P = 0.0031, 0.0011, and 0.0009, respectively). Moreover, the amount of most KRTs in EBC samples from LC patients showed a significant positive correlation with tumor size. Finally, we used a random forest algorithm to generate a robust model using EBC protein data for the diagnosis of patients with LC where the area under the ROC curve obtained indicated a good classification (82%). Thus this study demonstrates that the proteomic analysis of EBC samples is an appropriated approach to develop biomarkers for the diagnosis of lung cancer.

Diagnostic value of a pattern of exhaled breath condensate biomarkers in asthmatic children

2017 ◽  
Vol 45 (1) ◽  
pp. 2-10 ◽  
Author(s):  
I. Maloča Vuljanko ◽  
M. Turkalj ◽  
B. Nogalo ◽  
S. Bulat Lokas ◽  
D. Plavec
Keyword(s):  
Exhaled Breath Condensate ◽  
Diagnostic Value ◽  
Exhaled Breath ◽  
Asthmatic Children ◽  
Breath Condensate
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