scholarly journals Mass Spectrometric Identification of Proteins Enhanced by the Atomic Force Microscopy Immobilization Surface

2021 ◽  
Vol 22 (1) ◽  
pp. 431
Author(s):  
Anna L. Kaysheva ◽  
Pavel A. Frantsuzov ◽  
Arthur T. Kopylov ◽  
Tatyana O. Pleshakova ◽  
Alexander A. Stepanov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Ion Trap ◽  
Time Of Flight ◽  
Mass Spectrometric ◽  
Triple Quadrupole ◽  
Target Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mass Spectrometric Identification

An approach to highly-sensitive mass spectrometry detection of proteins after surface-enhanced concentrating has been elaborated. The approach is based on a combination of mass spectrometry and atomic force microscopy to detect target proteins. (1) Background: For this purpose, a technique for preliminary preparation of molecular relief surfaces formed as a result of a chemical or biospecific concentration of proteins from solution was developed and tested on several types of chip surfaces. (2) Methods: mass spectrometric identification of proteins using trailing detectors: ion trap, time of flight, orbital trap, and triple quadrupole. We used the electrospray type of ionization and matrix-assisted laser desorption/ionization. (3) Results: It is shown that when using locally functionalized atomically smooth surfaces, the sensitivity of the mass spectrometric method increases by two orders of magnitude as compared with measurements in solution. Conclusions: It has been demonstrated that the effective concentration of target proteins on specially prepared surfaces increases the concentration sensitivity of mass spectrometric detectors—time-of-flight, ion trap, triple quadrupole, and orbital ion trap in the concentration range from up to 10−15 M.

scholarly journals Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

2010 ◽  
Vol 56 (1) ◽  
pp. 26-39 ◽  
Author(s):  
A.L. Kaysheva ◽  
Yu.D. Ivanov ◽  
V.G. Zgoda ◽  
P.A. Frantsuzov ◽  
T.O. Pleshakova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spectrometric Identification ◽  
Detection And Identification ◽  
Viral Particles ◽  
Mass Spectrometric Identification

Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.

Cleanability of Soiled Stainless Steel as Studied by Atomic Force Microscopy and Time of Flight Secondary Ion Mass Spectrometry

2001 ◽  
Vol 64 (1) ◽  
pp. 87-93 ◽  
Author(s):  
R. D. BOYD ◽  
D. COLE ◽  
D. ROWE ◽  
J. VERRAN ◽  
A. J. PAUL ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Stainless Steel ◽  
Surface Defects ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Analytical Techniques ◽  
Force Microscopy ◽  
Atomic Force ◽  
Secondary Ion

The hygienic status of food contact surfaces can deteriorate with wear. Effective cleaning regimes must remove any adsorbed organic material as well as microorganisms. Previous work has determined the extent of surface wear occurring on a stainless steel surface within the food industry, and we have reproduced representative samples in vitro. Two surface analytical techniques, atomic force microscopy and time of flight secondary ion mass spectrometry were combined with fluorescence microscopy to give detailed analysis of stainless steel surfaces fouled with starch and milk powder, then cleaned with water either by a spray or brushing method. It was found that the surface cleanability is affected by the cleaning regime and the surface roughness, not only the average vertical roughness but also by the shape of the surface defects, with sharp scratches more difficult to clean than wider surface defects. Spray cleaning with distilled water was found to be a selective method by preferentially removing proteinaceous material more easily than fatty acid ester material. The analytical techniques employed provided information on selective cleanability and surface topography at a hitherto unexplored level, and the information gained may be of value in the design and investigation of novel cleaning regimes and hygienic surfaces.

scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals Salmon Muscle Adherence to Polymer Coatings and Determination of Antibiotic Residues by Reversed-Phase High-Performance Liquid Chromatography Coupled to Selected Reaction Monitoring Mass Spectrometry, Atomic Force Microscopy, and Fourier Transform Infrared Spectroscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
E. Zumelzu ◽  
M. J. Wehrhahn ◽  
O. Muñoz ◽  
F. Rull
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Urea Solution ◽  
Antibiotic Residues ◽  
Force Microscopy ◽  
Atomic Force

The persistent adhesion of salmon muscle to food container walls after treatment with urea solution was observed. This work evaluated the diffusion of antibiotics from the salmon muscle to the polyethylene terephthalate (PET) coating protecting the electrolytic chromium coated steel (ECCS) plates. New aquaculture production systems employ antibiotics such as florfenicol, florfenicol amine, oxytetracycline, and erythromycin to control diseases. The introduction of antibiotics is a matter of concern regarding the effects on human health and biodiversity. It is important to determine their impact on the adhesion of postmortem salmon muscle to can walls and the surface and structural changes affecting the functionality of multilayers. This work characterized the changes occurring in the multilayer PET polymer and steel of containers by electron microscopy, 3D atomic force microscopy (3D-AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. A robust mass spectrometry methodology was employed to determine the presence of antibiotic residues. No evidence of antibiotics was observed on the protective coating in the range between 0.001 and 2.0 ng/mL; however, the presence of proteins, cholesterol, and alpha-carotene was detected. This in-depth profiling of the matrix-level elements is relevant for the use of adequate materials in the canning export industry.

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