scholarly journals Alfombras de Guano: salvando nuestra herencia cultural

Esferas ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 26
Author(s):  
María Isabel Paz Suárez ◽  
Mónica Gabriela Zaldumbide Chiriboga
Keyword(s):  
San Francisco

El tejido de la alfombra anudada ha representado, por siglos, la identidad del cantón Guano, en Ecuador. Sin embargo, en la actualidad, la incapacidad de monetizar esta técnica artesanal ha estancado el desarrollo de su comunidad. La caída en ventas de las alfombras ha llevado a que el cantón entero comience a diversificar sus oficios, lo cual ha llevado a una pérdida de la herencia textil acumulada por siglos. Además, muchos de los artesanos, ante el golpe de la falta de demanda por sus actividades, han sufrido la explotación de intermediarios abusivos, la apropiación de sus oficios, remuneraciones injustas, entre otros problemas que alejan a las nuevas generaciones de aprender de estas tradiciones. Adicionalmente, la falta de formación adecuada para defender y adaptar sus oficios a un contexto contemporáneo enfrenta a las alfombras de Guano a una inminente extinción artesanal. Este ensayo es parte de una iniciativa de investigación y aplicación llamada “Alfombras de Guano: El Proyecto”, que surgió en 2017 del Colegio de Arquitectura y Diseño Interior (CADI), de la Universidad San Francisco de Quito. Desde entonces, se ha trabajado en conjunto con los artesanos en crear un plan a futuro basado en el cuarto de los objetivos de desarrollo sostenible de la ONU. Rediseñar las alfombras, capacitar a los artesanos y crear plataformas de exposición son los canales que se han implementado para revitalizar al cantón de Guano en el mediano y largo plazo. La aplicación exitosa de este plan tiene el potencial de convertirse en un caso de estudio para otras comunidades artesanales de la región, y contesta a la pregunta: ¿cómo se puede proteger el conocimiento artesanal y potencializarlo a través de la educación, para que sea un canal de desarrollo económico de pequeñas comunidades en la actualidad?

Ultrastructural Localization of Antigens by Capillary Action Immunocytochemistry

1996 ◽  
Vol 54 ◽  
pp. 40-41
Author(s):  
László G. Kömüves
Keyword(s):  
San Francisco ◽  
Colloidal Gold ◽  
Specific Binding ◽  
Ultrastructural Localization ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Adhesive Tape ◽  
Distilled Water ◽  
Capillary Action ◽  
Rabbit Igg

Light microscopic immunohistochemistry based on the principle of capillary action staining is a widely used method to localize antigens. Capillary action immunostaining, however, has not been tested or applied to detect antigens at the ultrastructural level. The aim of this work was to establish a capillary action staining method for localization of intracellular antigens, using colloidal gold probes.Post-embedding capillary action immunocytochemistry was used to detect maternal IgG in the small intestine of newborn suckling piglets. Pieces of the jejunum of newborn piglets suckled for 12 h were fixed and embedded into LR White resin. Sections on nickel grids were secured on a capillary action glass slide (100 μm wide capillary gap, Bio-Tek Solutions, Santa Barbara CA, distributed by CMS, Houston, TX) by double sided adhesive tape. Immunolabeling was performed by applying reagents over the grids using capillary action and removing reagents by blotting on filter paper. Reagents for capillary action staining were from Biomeda (Foster City, CA). The following steps were performed: 1) wet the surface of the sections with automation buffer twice, 5 min each; 2) block non-specific binding sites with tissue conditioner, 10 min; 3) apply first antibody (affinity-purified rabbit anti-porcine IgG, Sigma Chem. Co., St. Louis, MO), diluted in probe diluent, 1 hour; 4) wash with automation buffer three times, 5 min each; 5) apply gold probe (goat anti-rabbit IgG conjugated to 10 nm colloidal gold, Zymed Laboratories, South San Francisco, CA) diluted in probe diluent, 30 min; 6) wash with automation buffer three times, 5 min each; 7) post-fix with 5% glutaraldehyde in PBS for 10 min; 8) wash with PBS twice, 5 min each; 9) contrast with 1% OSO4 in PBS for 15 min; 10) wash with PBS followed by distilled water for5 min each; 11) stain with 2% uranyl acetate for 10 min; 12) stain with lead citrate for 2 min; 13) wash with distilled water three times, 1 min each. The glass slides were separated, and the grids were air-dried, then removed from the adhesive tape. The following controls were used to ensure the specificity of labeling: i) omission of the first antibody; ii) normal rabbit IgG in lieu of first antibody; iii) rabbit anti-porcine IgG absorbed with porcine IgG.

Game Changer

2011 ◽  
Vol 20 (1) ◽  
pp. 17-18 ◽  
Author(s):  
Lateef McLeod
Keyword(s):  
San Francisco ◽  
Mobile Technology ◽  
Technology Tools ◽  
Communication Challenges ◽  
Communication Needs ◽  
Game Changer

Abstract Individuals with significant communication challenges need to communicate across many different venues. The author, from the perspective of an individual who uses AAC, discusses the strengths and weaknesses of both traditional AAC technologies and new mobile AAC technologies. He describes how access to AAC has allowed him to fulfill his dreams as a presenter and writer. He successfully manages a blog in San Francisco, writes grants, and has published his first book of poetry. Not one AAC device fits all of his communication needs; however, access to mobile technology tools has increased his flexibility across environments and given him another successful tool for communication.

126: Nephrectomy for Traumatic Renal Injuries: 26 Year Experience at San Francisco General Hospital

2005 ◽  
Vol 173 (4S) ◽  
pp. 34-34
Author(s):  
Viraj A. Master ◽  
Jennifer Young ◽  
Jack W. McAninch
Keyword(s):  
General Hospital ◽  
San Francisco ◽  
Renal Injuries
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