scholarly journals Identification of larval salivary gland polytene chromosomes of the peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae)

2019 ◽  
Vol 33 (3) ◽  
pp. 295-302
Author(s):  
Yukihiro Momota ◽  
Hideyuki Takano ◽  
Masayuki Azuma ◽  
◽  
◽  
...  
Keyword(s):  
Salivary Gland ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Peach Fruit ◽  
Bactrocera Zonata ◽  
Larval Salivary Gland

Genetic Analysis of the Drosophila 63F Early Puff: Characterization of Mutations in E63-1 and maggie, a Putative Tom22

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 229-244
Author(s):  
Martina Vaskova ◽  
A M Bentley ◽  
Samantha Marshall ◽  
Pamela Reid ◽  
Carl S Thummel ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Genetic Analysis ◽  
Polytene Chromosomes ◽  
Reading Frame ◽  
Protein Levels ◽  
Ef Hand ◽  
Larval Salivary Gland ◽  
Complementation Groups ◽  
Inducible Genes ◽  
Early Puff

Abstract The 63F early puff in the larval salivary gland polytene chromosomes contains the divergently transcribed E63-1 and E63-2 ecdysone-inducible genes. E63-1 encodes a member of the EF-hand family of Ca2+-binding proteins, while E63-2 has no apparent open reading frame. To understand the functions of the E63 genes, we have determined the temporal and spatial patterns of E63-1 protein expression, as well as undertaken a genetic analysis of the 63F puff. We show that E63-1 is expressed in many embryonic and larval tissues, but the third-instar larval salivary gland is the only tissue where increases in protein levels correlate with increases in ecdysone titer. Furthermore, the subcellular distribution of E63-1 protein changes dynamically in the salivary glands at the onset of metamorphosis. E63-1 and E63-2 null mutations, however, have no effect on development or fertility. We have characterized 40 kb of the 63F region, defined as the interval between Ubi-p and E63-2, and have identified three lethal complementation groups that correspond to the dSc-2, ida, and mge genes. We show that mge mutations lead to first-instar larval lethality and that Mge protein is similar to the Tom22 mitochondrial import proteins of fungi, suggesting that it has a role in mitochondrial function.

A modification of Heidenhain's iron-haematoxylin technique, as used to stain smears of Drosophila larval polytene chromosomes and neuroblast cells

1971 ◽  
Vol 49 (1) ◽  
pp. 132-133 ◽  
Author(s):  
Albert E. Moorman
Keyword(s):  
Acetic Acid ◽  
Salivary Gland ◽  
Methyl Alcohol ◽  
Polytene Chromosomes ◽  
Larval Salivary Gland

Acetic-acid-fixed smears of Drosophila larval salivary gland chromosomes and of neuroblast cells from the larval ganglion undergoing mitosis are prepared by a modification of Heidenhain's iron-haematoxylin technique, in which absolute methyl alcohol is the solvent of all reagents used in the staining process.

Genetic and cytogenetic analysis of the fruit fly Rhagoletis cerasi (Diptera: Tephritidae)

Genome ◽  
2008 ◽  
Vol 51 (7) ◽  
pp. 479-491 ◽  
Author(s):  
Ilias Kounatidis ◽  
Nikolaos Papadopoulos ◽  
Kostas Bourtzis ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Sex Chromosomes ◽  
Cytogenetic Analysis ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Pest Species ◽  
Heteromorphic Sex Chromosomes ◽  
Weak Points ◽  
Heterogametic Sex ◽  
Rhagoletis Cerasi

The European cherry fruit fly, Rhagoletis cerasi , is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. We report here a cytogenetic analysis of 4 natural Greek populations of R. cerasi, all of them infected with the endosymbiotic bacterium Wolbachia pipientis . The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. The mitotic metaphase complement consists of 6 pairs of chromosomes, including one pair of heteromorphic sex chromosomes, with the male being the heterogametic sex. The analysis of the salivary gland polytene complement has shown a total of 5 long chromosomes (10 polytene arms) that correspond to the 5 autosomes of the mitotic nuclei and a heterochromatic mass corresponding to the sex chromosomes. The most prominent landmarks of each polytene chromosome, the “weak points”, and the unusual asynapsis of homologous pairs of polytene chromosomes at certain regions of the polytene elements are also presented and discussed.

Bactrocera zonata (peach fruit fly).

2021 ◽  
Author(s):  
Abdeljelil Bakri
Keyword(s):  
Plant Protection ◽  
Reproductive Potential ◽  
Fruit Fly ◽  
Dispersal Ability ◽  
South East Asia ◽  
Peach Fruit ◽  
Export Markets ◽  
Bactrocera Zonata ◽  
Control Programmes ◽  
Mediterranean Plant

Abstract Native to South and South-East Asia, B. zonata is now found in more than 20 countries. The potential risk of its introduction to a new area is facilitated by increasing international tourism and trade, and is influenced by changes in climate and land use. After introduction, it can easily adapt and spread as it is a polyphagous species and has a high reproductive potential (as many as 564 eggs in a lifetime), high biotic potential (several generations of progeny in a year), and a rapid dispersal ability. B. zonata is a strong flier and can be active throughout the year. Economic impacts may result primarily from the loss of export markets and the costly requirement of quarantine restrictions and eradication measures. Furthermore, its establishment may have a serious impact on the environment following the initiation of chemical and/or biological control programmes. B. zonata is of quarantine significance to EPPO (the European and Mediterranean Plant Protection Organization) countries. The pest is classified on the A1 List of Pests recommended for regulation as quarantine pests.

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes

1988 ◽  
Vol 8 (5) ◽  
pp. 1877-1886
Author(s):  
B M Benton ◽  
S Berrios ◽  
P A Fisher
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Transcriptional Regulation ◽  
Salivary Gland ◽  
Indirect Immunofluorescence ◽  
Polytene Chromosomes ◽  
Nuclear Interior ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Larval Salivary Gland

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

Assessment Effect of Gamma Radiation on the Flight Ability of the Peach Fruit Fly, Bactrocera zonata (Saunders)

2013 ◽  
Vol 16 (23) ◽  
pp. 1730-1736
Author(s):  
I.R. El-Gendy ◽  
M.A.M. El-Aw ◽  
A.G. Hashem ◽  
K.A. Draz
Keyword(s):  
Gamma Radiation ◽  
Fruit Fly ◽  
Peach Fruit ◽  
Flight Ability ◽  
Bactrocera Zonata

The genome of the olive fruit fly Bactrocera oleae: localization of molecular markers by in situ hybridization to the salivary gland polytene chromosomes

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 744-751 ◽  
Author(s):  
Anna Zambetaki ◽  
Antigone Zacharopoulou ◽  
Zacharias G. Scouras ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Molecular Markers ◽  
In Situ Hybridization ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Bactrocera Oleae ◽  
Olive Fruit Fly ◽  
Olive Fruit
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