Differences of alpha3beta1 integrin glycans from different human bladder cell lines.

Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of alpha3beta1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of alpha3 and beta1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines alpha3beta1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in beta1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of beta1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in beta1 subunit from Hu456 transitional cell cancer line. On the other hand alpha3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with alpha3 not beta1 subunit.

Download Full-text

Expression of beta1-integrins and N-cadherin in bladder cancer and melanoma cell lines.

Acta Biochimica Polonica ◽

10.18388/abp.2000_3968 ◽

2000 ◽

Vol 47 (4) ◽

pp. 1159-1170 ◽

Cited By ~ 25

Author(s):

P Laidler ◽

D Gil ◽

A Pituch-Noworolska ◽

D Ciołczyk ◽

D Ksiazek ◽

...

Keyword(s):

Bladder Cancer ◽

Cell Line ◽

Cell Lines ◽

Melanoma Cell ◽

Cell Cancer ◽

Transitional Cell ◽

Melanoma Cells ◽

Integrin Subunit ◽

Bladder Cell ◽

Transitional Cell Cancer

Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of alpha5 (P < 0.001), alpha2 and beta1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of beta1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of alpha2, alpha3, and alpha5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of alpha2, alpha3 and alpha5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and alpha5 integrin subunit seems to be associated with the most invasive melanoma phenotype.

Download Full-text

Adriamycin (ADM) induced apoptosis in Transitional Cell Cancer (TCC) cell lines accompanied by p21 WAF1/CIP1 induction

APOPTOSIS ◽

10.1023/a:1026472700554 ◽

1997 ◽

Vol 2 (2) ◽

pp. 207-213 ◽

Cited By ~ 8

Author(s):

V. N. Bilim ◽

Y. Tomita ◽

T. Kawasaki ◽

M. Takeda ◽

K. Takahashi

Keyword(s):

Cell Lines ◽

Cell Cancer ◽

Transitional Cell ◽

Transitional Cell Cancer ◽

P21 Waf1 ◽

Induced Apoptosis

Download Full-text

660 Effect of hexyl aminolevulinate on antitumor activity of apaziquone in human bladder cell lines

European Urology Supplements ◽

10.1016/s1569-9056(12)60657-5 ◽

2012 ◽

Vol 11 (1) ◽

pp. e660-e660a

Author(s):

G. Reddy ◽

S. Chawla ◽

S. Fruchtman

Keyword(s):

Antitumor Activity ◽

Cell Lines ◽

Human Bladder ◽

Bladder Cell

Download Full-text

Cytotoxic Necrotizing Factor Type 1 of Uropathogenic Escherichia coli Kills Cultured Human Uroepithelial 5637 Cells by an Apoptotic Mechanism

Infection and Immunity ◽

10.1128/iai.68.10.5869-5880.2000 ◽

2000 ◽

Vol 68 (10) ◽

pp. 5869-5880 ◽

Cited By ~ 80

Author(s):

Melody Mills ◽

Karen C. Meysick ◽

Alison D. O'Brien

Keyword(s):

Escherichia Coli ◽

Cell Line ◽

Cell Lines ◽

Human Bladder ◽

E Coli ◽

Cytotoxic Necrotizing Factor ◽

Bladder Cell ◽

Factor Type ◽

Tract Infections

ABSTRACT Pathogenic Escherichia coli associated with urinary tract infections (UTIs) in otherwise healthy individuals frequently produce cytotoxic necrotizing factor type 1 (CNF1), a member of the family of bacterial toxins that target the Rho family of small GTP-binding proteins. To gain insight into the function of CNF1 in the development of E. coli-mediated UTIs, we examined the effects of CNF1 intoxication on a panel of human cell lines derived from physiologically relevant sites (bladder, ureters, and kidneys). We identified one uroepithelial cell line that exhibited a distinctly different CNF1 intoxication phenotype from the prototypic one of multinucleation without cell death that is seen when HEp-2 or other epithelial cells are treated with CNF1. The 5637 bladder cell line detached from the growth surface within 72 h of CNF1 intoxication, a finding that suggested frank cytotoxicity. To determine the basis for the unexpected toxic effect of CNF1 on 5637 cells, we compared the degree of toxin binding, actin fiber formation, and Rho modification with those CNF1-induced events in HEp-2 cells. We found no apparent difference in the amount of CNF1 bound to 5637 cells and HEp-2 cells. Moreover, CNF1 modified Rho, in vivo and in vitro, in both cell types. In contrast, one of the classic responses to CNF1 in HEp-2 and other epithelial cell lines, the formation of actin stress fibers, was markedly absent in 5637 cells. Indeed, actin stress fiber induction by CNF1 did not occur in any of the other human bladder cell lines that we tested (J82, SV-HUC-1, or T24). Furthermore, the appearance of lamellipodia and filopodia in 5637 cells suggested that CNF1 activated the Cdc42 and Rac proteins. Finally, apoptosis was observed in CNF1-intoxicated 5637 cells. If our results with 5637 cells reflect the interaction of CNF1 with the transitional uroepithelium in the human bladder, then CNF1 may be involved in the exfoliative process that occurs in that organ after infection with uropathogenic E. coli.

Download Full-text

Angiogenesis-inducing ability of human bladder epithelium cell lines and ‘spontaneously’ transformed murine fibroblasts

European Journal of Cancer and Clinical Oncology ◽

10.1016/0277-5379(83)90283-3 ◽

1983 ◽

Vol 19 (9) ◽

pp. 1293

Keyword(s):

Cell Lines ◽

Bladder Epithelium ◽

Human Bladder ◽

Epithelium Cell ◽

Murine Fibroblasts

Download Full-text

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

Cytometry Part A ◽

10.1002/cyto.a.20329 ◽

2006 ◽

Vol 69A (10) ◽

pp. 1077-1085 ◽

Cited By ~ 6

Author(s):

Nor F. Rajab ◽

Declan J. McKenna ◽

Jim Diamond ◽

Kate Williamson ◽

Peter W. Hamilton ◽

...

Keyword(s):

Cell Lines ◽

Nuclear Chromatin ◽

Human Bladder ◽

Bladder Cell

Download Full-text

The ganglioside profile of human bladder cell lines and the effect of exogenous gangliosides on their growth

International Journal of Oncology ◽

10.3892/ijo.8.2.275 ◽

1996 ◽

Author(s):

M Slevin ◽

S Kumar ◽

J Gaffney

Keyword(s):

Cell Lines ◽

Human Bladder ◽

Bladder Cell

Download Full-text

Studies of the biosynthesis of the mannose 6-phosphate receptor in receptor-positive and -deficient cell lines.

The Journal of Cell Biology ◽

10.1083/jcb.97.6.1700 ◽

1983 ◽

Vol 97 (6) ◽

pp. 1700-1706 ◽

Cited By ~ 21

Author(s):

D E Goldberg ◽

C A Gabel ◽

S Kornfeld

Keyword(s):

Cell Lines ◽

Lysosomal Enzymes ◽

Cell Types ◽

Receptor Protein ◽

Complex Type ◽

Cultured Cell ◽

Selective Targeting ◽

High Mannose ◽

Mannose 6 Phosphate ◽

Intracellular Pathway

Phosphomannosyl residues present on lysosomal enzymes are specifically recognized by the mannose 6-phosphate receptor protein. This interaction results in the selective targeting of lysosomal enzymes to lysosomes. While this pathway is operative in many cell types, we have found four cultured cell lines that are deficient in the ability to bind lysosomal enzymes containing phosphomannosyl residues to their intracellular or surface membranes (Gabel, C., D. Goldberg, and S. Kornfeld, 1983, Proc. Natl. Acad. Sci. USA, 80:775-779). These cells appear to segregate lysosomal enzymes by an alternate intracellular pathway. To determine the basis for the lack of mannose 6-phosphate receptor activity in these cell lines, we studied the biosynthesis of the receptor in receptor-positive (BW5147) and receptor-deficient (P388D1 and MOPC 315) cells. The cells were labeled with [2-3H]mannose or [35S]methionine and the receptor was immunoprecipitated with an antireceptor antiserum. BW5147 cells synthesize a receptor protein whose size increases after translation/glycosylation. MOPC 315 cells produce an apparently normal receptor and degrade it rapidly. P388D1 cells fail to synthesize any detectable receptor. The receptor from BW5147 and MOPC 315 cells is a glycoprotein with both high mannose and complex asparagine-linked oligosaccharides. The complex-type units become fully sialylated and remain so during long periods of chase.

Download Full-text