scholarly journals The Direct Influence of Cytomegalovirus Lysate on the Natural Killer Cell Receptor Repertoire

2021 ◽  
Author(s):  
Saeede Soleimanian ◽  
Ramin Yaghobi ◽  
Mohammad Hossein Karimi ◽  
Bita Geramizadeh ◽  
Jamshid Roozbeh
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Receptor ◽  
Direct Influence ◽  
Receptor Repertoire ◽  
Adaptive Capabilities ◽  
Nk Cell Receptor ◽  
Cell Receptor Repertoire

Natural killer (NK) cells are essential for controlling certain viral infections, including cytomegalovirus (CMV). In particular, the importance of NK cells in the context of CMV infection is underscored by the adaptive capabilities of these cells. Evidence suggests that some viruses can directly interfere with NK cell compartments and their activation and lead to shape-shifting the NK cell receptor repertoire. Still, it remains unknown whether the CMV can interact with NK cells without intermediaries. Here, we examined whether the direct effects of CMV lysate alter phenotypical properties of NK cells. To investigate this issue, NK cells were isolated from the blood of CMV seropositive healthy donors by negative magnetic separation. Isolated NK cells were cultured in the presence of CMV lysate and analyzed for the expression of NKG2A, NKG2C, and CD57 by FACS caliber. The results showed that NKG2C expression is significantly upregulated in the presence of CMV lysate compared to without stimulated group (mean increase, 6.65 %; 95% CI, 0.2582 to 13.02; p=0.043; R square: 0.38). Likewise, results have shown a significant decrease in the frequency of NKG2A+CD57- NK cell subsets (p=0.005; 95% CI, -13.49 to -3.151; R square: 0.5957) in the stimulated group compared to without stimulated ones. According to these results, CMV may drive a direct influence on NK cell receptor repertoire, including the expansion of NK cells expressing NKG2C receptor, which is needed for further studies.

KLRL1, a novel killer cell lectinlike receptor, inhibits natural killer cell cytotoxicity

Blood ◽  
2004 ◽  
Vol 104 (9) ◽  
pp. 2858-2866 ◽  
Author(s):  
Yanmei Han ◽  
Minghui Zhang ◽  
Nan Li ◽  
Taoyong Chen ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Receptor ◽  
Sh2 Domain ◽  
Target Cells ◽  
Lymphoid Tissues ◽  
Nk Cell Receptor ◽  
Human And Mouse

Abstract Natural killer (NK) cell inhibitory receptors play important roles in the regulation of target susceptibility to natural killing. Here, we report the molecular cloning and functional characterization of a novel NK cell receptor, KLRL1, from human and mouse dendritic cells. KLRL1 is a type II transmembrane protein with an immunoreceptor tyrosine-based inhibitory motif and a C-type lectinlike domain. The KLRL1 gene is located in the central region of the NK gene complex in both humans and mice, on human chromosome 12p13 and mouse chromosome 6F3, adjacent to the other KLR genes. KLRL1 is preferentially expressed in lymphoid tissues and immune cells, including NK cells, T cells, dendritic cells, and monocytes or macrophages. Western blot and fluorescence confocal microscopy analyses indicated that KLRL1 is a membrane-associated glycoprotein, which forms a heterodimer with an as yet unidentified partner. Human and mouse KLRL1 are both predicted to contain putative immunoreceptor tyrosine-based inhibitory motifs (ITIMs), and immunoprecipitation experiments demonstrated that KLRL1 associates with the tyrosine phosphatases SHP-1 (SH2-domain-containing protein tyrosine phosphatase 1) and SHP-2. Consistent with its potential inhibitory function, pretreatment of target cells with human KLRL1-Fc fusion protein enhances NK-mediated cytotoxicity. Taken together, our results demonstrate that KLRL1 belongs to the KLR family and is a novel inhibitory NK cell receptor.

scholarly journals Sequential recovery of NK cell receptor repertoire after allogeneic hematopoietic SCT

2010 ◽  
Vol 45 (6) ◽  
pp. 1022-1030 ◽  
Author(s):  
S Giebel ◽  
J Dziaczkowska ◽  
T Czerw ◽  
J Wojnar ◽  
M Krawczyk-Kulis ◽  
...  
Keyword(s):  
Nk Cell ◽  
Cell Receptor ◽  
Receptor Repertoire ◽  
Nk Cell Receptor ◽  
Cell Receptor Repertoire

The NK cell receptor repertoire: formation, adaptation and exploitation

2003 ◽  
Vol 15 (2) ◽  
pp. 233-237 ◽  
Author(s):  
Werner Held ◽  
Jérôme D Coudert ◽  
Jacques Zimmer
Keyword(s):  
Nk Cell ◽  
Cell Receptor ◽  
Receptor Repertoire ◽  
Nk Cell Receptor ◽  
Cell Receptor Repertoire

scholarly journals The Role of Natural Killer (NK) Cells and NK Cell Receptor Polymorphisms in the Assessment of HIV-1 Neutralization

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e29454 ◽  
Author(s):  
Bruce K. Brown ◽  
Lindsay Wieczorek ◽  
Gustavo Kijak ◽  
Kara Lombardi ◽  
Jeffrey Currier ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Cell Receptor ◽  
Nk Cell Receptor ◽  
Hiv 1

scholarly journals NKB1: a natural killer cell receptor involved in the recognition of polymorphic HLA-B molecules.

1994 ◽  
Vol 180 (2) ◽  
pp. 537-543 ◽  
Author(s):  
V Litwin ◽  
J Gumperz ◽  
P Parham ◽  
J H Phillips ◽  
L L Lanier
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Cell Clone ◽  
Killer Cell ◽  
Cell Receptor ◽  
Target Cells ◽  
Multiple Alleles ◽  
Barr Virus ◽  
Cell Clones

Natural killer (NK) cells kill normal and transformed hematopoietic cells that lack expression of major histocompatibility complex (MHC) class I antigens. Lysis of HLA-negative Epstein Barr virus-transformed B lymphoblastoid cell lines (B-LCL) by human NK cell clones can be inhibited by transfection of the target cells with certain HLA-A, -B, or -C alleles. NK cell clones established from an individual demonstrate clonal heterogeneity in HLA recognition and a single NK clone can recognize multiple alleles. We describe a potential human NK cell receptor (NKB1) for certain HLA-B alleles (e.g., HLA-B*5101 and-B*5801) identified by the mAb DX9. NKB1 is a 70-kD glycoprotein that is expressed on a subset of NK cells and NK cell clones. DX9 monoclonal antibody (mAb) specifically inhibits the interaction between NK cell clones and B-LCL targets transfected with certain HLA-B alleles, but does not affect recognition of HLA-A or HLA-C antigens. An individual NK cell clone can independently recognize B-LCL targets transfected with HLA-B or HLA-C antigens; however, DX9 mAb only affects interaction with transfectants expressing certain HLA-B alleles. These findings demonstrate the existence of NK cell receptors involved in the recognition of HLA-B and imply the presence of multiple receptors for MHC on an individual NK clone.

scholarly journals NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity.

1995 ◽  
Vol 181 (5) ◽  
pp. 1911-1915 ◽  
Author(s):  
J C Ryan ◽  
E C Niemi ◽  
M C Nakamura ◽  
W E Seaman
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Receptor ◽  
Specific Receptor ◽  
Natural Killer Cell Cytotoxicity ◽  
Natural Killing ◽  
Inositol 1,4,5 Trisphosphate ◽  
Functional Features

NKR-P1A is a lectinlike surface molecule expressed on rat natural killer (NK) cells. NKR-P1A has structural and functional features of an activating NK cell receptor, but a requirement for NKR-P1A in target cell lysis has not been determined. To define the role of NKR-P1A in natural killing, we have generated a mutant of the rat NK cell line, RNK-16, lacking expression of all members of the NKR-P1 receptor family. Although these NKR-P1-deficient NK cells were able to kill many standard tumor targets, including YAC-1, they were selectively deficient in the lysis of IC-21 macrophage, B-16 melanoma, and C1498 lymphoma targets. Reexpression of a single member of the NKR-P1 family, NKR-P1A, on mutant cells restored lysis of IC-21, and killing of IC-21 targets through rat NKR-P1A was completely blocked by F(ab')2 anti-NKR-P1A. Reexpression of NKR-P1A also restored transmembrane signaling to IC-21, as assessed by the generation of inositol-1,4,5-trisphosphate. The generation of inositol-1,4,5-trisphosphate was also restored in response to B-16 targets, but both B-16 and C1498 cells remained resistant to lysis, indicating that other NK cell molecules, perhaps within the NKR-P1 family, are required for the efficient killing of these tumors. These results are the first to demonstrate that NKR-P1A is a target-specific receptor that activates natural killing.

Correction: Influence of human cytomegalovirus infection on the NK cell receptor repertoire in children

2010 ◽  
Vol 41 (1) ◽  
pp. 258-258
Author(s):  
Adriana Monsiváis-Urenda ◽  
Daniel Noyola-Cherpitel ◽  
Alba Hernández-Salinas ◽  
Christian García-Sepúlveda ◽  
Neus Romo ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Nk Cell ◽  
Cell Receptor ◽  
Receptor Repertoire ◽  
Human Cytomegalovirus Infection ◽  
Nk Cell Receptor ◽  
Cell Receptor Repertoire

scholarly journals Influence of human cytomegalovirus infection on the NK cell receptor repertoire in children

2010 ◽  
Vol 40 (5) ◽  
pp. 1418-1427 ◽  
Author(s):  
Adriana Monsiváis‐Urenda ◽  
Daniel Noyola‐Cherpitel ◽  
Alba Hernández‐Salinas ◽  
Christian García‐Sepúlveda ◽  
Neus Romo ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Nk Cell ◽  
Cell Receptor ◽  
Receptor Repertoire ◽  
Human Cytomegalovirus Infection ◽  
Nk Cell Receptor ◽  
Cell Receptor Repertoire

scholarly journals Pseudorabies Virus US3 Protein Kinase Protects Infected Cells from NK Cell-Mediated Lysis via Increased Binding of the Inhibitory NK Cell Receptor CD300a

2015 ◽  
Vol 90 (3) ◽  
pp. 1522-1533 ◽  
Author(s):  
K. Grauwet ◽  
M. Vitale ◽  
S. De Pelsmaeker ◽  
T. Jacob ◽  
K. Laval ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Nk Cells ◽  
Natural Killer ◽  
Pseudorabies Virus ◽  
Nk Cell ◽  
Cell Receptor ◽  
Target Cells ◽  
Group I ◽  
Infected Cells ◽  
Nk Cell Receptor

ABSTRACTSeveral reports have indicated that natural killer (NK) cells are of particular importance in the innate response against herpesvirus infections. As a consequence, herpesviruses have developed diverse mechanisms for evading NK cells, although few such mechanisms have been identified for the largest herpesvirus subfamily, the alphaherpesviruses. The antiviral activity of NK cells is regulated by a complex array of interactions between activating/inhibitory receptors on the NK cell surface and the corresponding ligands on the surfaces of virus-infected cells. Here we report that the US3 protein kinase of the alphaherpesvirus pseudorabies virus (PRV) displays previously uncharacterized immune evasion properties: it triggers the binding of the inhibitory NK cell receptor CD300a to the surface of the infected cell, thereby providing increased CD300a-mediated protection of infected cells against NK cell-mediated lysis. US3-mediated CD300a binding was found to depend on aminophospholipid ligands of CD300a and on group I p21-activated kinases. These data identify a novel alphaherpesvirus strategy for evading NK cells and demonstrate, for the first time, a role for CD300a in regulating NK cell activity upon contact with virus-infected target cells.IMPORTANCEHerpesviruses have developed fascinating mechanisms to evade elimination by key elements of the host immune system, contributing to their ability to cause lifelong infections with recurrent reactivation events. Natural killer (NK) cells are central in the innate antiviral response. Here we report that the US3 protein kinase of the alphaherpesvirus pseudorabies virus displays a previously uncharacterized capacity for evasion of NK cells. Expression of US3 protects infected cells from NK cell-mediated lysis via increased binding of the inhibitory NK cell receptor CD300a. We show that this US3-mediated increase in CD300a binding depends on aminophospholipids and on cellular p21-activated kinases (PAKs). The identification of this novel NK cell evasion strategy may contribute to the design of improved herpesvirus vaccines and may also have significance for other PAK- and CD300a-modulating viruses and cancer cells.

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