scholarly journals Antiviral Activity Effect of Silver Nanoparticles (Agnps) Solution Against the Growth of Infectious Bursal Disease Virus on Embryonated Chicken Eggs with Elisa Test

2017 ◽  
Vol 3 (6) ◽  
pp. 536 ◽  
Author(s):  
Rosa Pangestika ◽  
Rahaju Ernawati
Keyword(s):  
Silver Nanoparticles ◽  
Antiviral Activity ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
Significant Difference ◽  
Bursal Disease ◽  
Chicken Eggs ◽  
Embryonated Chicken

Infectious bursal disease virus is one of the strategic infectious disease in Indonesia. Despite disinfection and vaccination technology has been doing, the cases still frequently occur and it needs another alternative technology to be developed to against IBD virus. This research try to answer the problem, it examines the effect of antiviral activity of silver nanoparticles (AgNPs) solution against the growth of infectious bursal disease virus in embryonated chicken eggs with ELISA Test. The research has two methods, the first method is conducted by mixing a solution of AgNPs and IBD Virus, two hours before inoculated (preventive method) and the second method is the virus inoculated first, 48 hours later the AgNPs solution injected (therapy method). Each method has several dosage of AgNPs solution respectively 0 ppm (positive control 20 ppm, 40 ppm, and 50 ppm). Virus samples taken from the choriallantoic membrane (CAM) and the embryo by crushed method. Results based on the value of OD (optical density) ELISA Test and Statistical Test ANOVA General Linier Models Univariate with Post-Hoc Duncan 5%, both methods have no significant difference (p>0.05), it means the solution of AgNPs has good preventative and therapeutic characteristic. The mean of OD values also showed dosage of 20ppm is most effective dosage in against the growth of the virus, the dosage has significant difference (p<0.05). The decreasing amount of virus in CAM and in embryos were not significantly different (p>0.05), in both CAM and embryo AgNPs solution has good antiviral properties. Keywords : Silver Nanoparticles, Antiviral, Infectious Bursal Disesase, ELISA, Embryonated Chicken Eggs

scholarly journals Evaluation of secondary metabolites of herbal plant extracts as an antiviral effect on infectious bursal disease virus isolates in embryonated chicken eggs

2021 ◽  
pp. 2971-2978
Author(s):  
Rawaa Saladdin Jumaa ◽  
Dhuha Ismael Abdulmajeed ◽  
Abdulkarim Jafar Karim
Keyword(s):  
Viral Infection ◽  
Disease Virus ◽  
Plant Extracts ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Post Inoculation ◽  
Pathological Changes ◽  
Bursal Disease ◽  
Chicken Eggs ◽  
Embryonated Chicken

Background and Aim: Infectious bursal disease attacks the poultry industry, mainly young chickens, causing immunosuppression, and death with high economic losses. This study aimed to evaluate the effects of the monoextract, diextracts, and triextracts of Quercus infectoria (QI), Citrus aurantifolia (CiA), and Coffea arabica (CoA) on infectious bursal disease virus (IBDV) in embryonated chicken eggs (ECEs). Materials and Methods: The experimental design consisted of three sets of ECEs at 11 days of age, and each set included seven groups (G1-G7). The extracts of QI, CiA, and CoA were inoculated to ECEs by the chorioallantoic membrane method before, in concomitant (mixed) with, and after IBDV infection to the first, second, and third sets, respectively. The monoextract, diextracts, and triextracts of QI, CiA, and CoA were given at 1%, 2%, 5%, and 10% concentrations to G1-G3, G4-G6, and G7, respectively. Real-time polymerase chain reaction identified and confirmed the virus in accordance with the pathological changes. Results: The monoextract (5-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas the monoextract (10% concentration) inhibited IBDV during mixed inoculation and post-inoculation. Diextracts (2-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas diextracts (5-10% concentrations) inhibited IBDV during mixed inoculation and post-inoculation. Triextracts (1%, 2%, 5%, and 10% concentrations) inhibited IBDV by ameliorating the pathological changes of the virus and preventing the death of ECEs. Conclusion: The inoculation of herbal extracts, particularly triextracts, alleviates the pathological changes in ECEs infected with IBDV. This study recommends the oral route in evaluating plant extracts against IBDV in poultry.

scholarly journals Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate

2006 ◽  
Vol 87 (1) ◽  
pp. 209-216 ◽  
Author(s):  
Cyril Le Nouën ◽  
Gaëlle Rivallan ◽  
Didier Toquin ◽  
Pierre Darlu ◽  
Yannick Morin ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Virus Production ◽  
Infectious Bursal Disease ◽  
Naturally Occurring ◽  
Significant Difference ◽  
Bursal Disease ◽  
Difference Test ◽  
Post Infectious

The purpose of this study was to compare the molecular epidemiology of infectious bursal disease virus (IBDV) segments A and B of 50 natural or vaccine IBDV strains that were isolated or produced between 1972 and 2002 in 17 countries from four continents, with phenotypes ranging from attenuated to very virulent (vv). These strains were subjected to sequence and phylogenetic analysis based on partial sequences of genome segments A and B. Although there is co-evolution of the two genome segments (70 % of strains kept the same genetic relatives in the segment A- and B-defined consensus trees), several strains (26 %) were identified with the incongruence length difference test as exhibiting a significantly different phylogenetic relationship depending on which segment was analysed. This suggested that natural reassortment could have occurred. One of the possible naturally occurring reassortant strains, which exhibited a segment A related to the vvIBDV cluster whereas its segment B was not, was thoroughly sequenced (coding sequence of both segments) and submitted to a standardized experimental characterization of its acute pathogenicity. This strain induced significantly less mortality than typical vvIBDVs; however, the mechanisms for this reduced pathogenicity remain unknown, as no significant difference in the bursal lesions, post-infectious antibody response or virus production in the bursa was observed in challenged chickens.

scholarly journals Adjuvanticity of sliver nanoparticles with Infectious bursal disease virus VP2 protein and its effect on humoral immune response in chickens

2021 ◽  
Author(s):  
Sudhakar P. Awandkar ◽  
Prabhakar A Tembhurne ◽  
Nitin V Kurkure ◽  
Sandeep P Chaudhari ◽  
Sachin W Bonde ◽  
...  
Keyword(s):  
Immune Response ◽  
Silver Nanoparticles ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Gene Segment ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Humoral Immune ◽  
Bursal Disease

Abstract The present study was conducted to decipher the effect of silver nanoparticles conjugated marker recombinant VP2 immunogen of Infectious bursal disease virus on humoral immune response in chickens. The hypervariable VP2 gene segment of field Infectious bursal disease virus, consisting of major and minor hydrophilic loops, was amplified using reverse transcription-polymerase chain reaction. The gene segment of size 664 bp was cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated recombinant VP2 protein (rVP2, 19 kDa) was expressed in a prokaryotic expression system using Escherichia coli BL32DE3 cells. The rVP2 protein showed reactivity with specific anti VP2 chicken antibodies. The results of Western blot revealed its utility in serological diagnosis. The recombinant antigen was tested for immunogenic potential by vaccinating the chickens with and without silver nanoparticles. The rVP2 protein blended with adjuvant grade montanide oil showed a highly significant rise in serum IgY titers. The titers induced by rVP2 protein mixed with montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and silver nanoparticles than in chickens vaccinated with conventional vaccines or rVP2 protein. The results represent Infectious bursal disease virus rVP2 protein as a promising candidate for the DIVA vaccine and sero-diagnostic tool. For the first time, the current study elucidated the adjuvanticity effect of silver nanoparticles on avian Infectious bursal disease virus rVP2 vaccine potency.

scholarly journals Two Patented Probiotics Mitigated the Haematological Alterations Induced by a Very Virulent Infectious Bursal Disease Virus in Chicks

2021 ◽  
Author(s):  
Aliyu Danlami Andamin ◽  
Paul Ayuba Abdu ◽  
Felix Tersua Akade ◽  
Ochuko Orakpoghenor ◽  
Tagang Aluwong
Keyword(s):  
Blood Cell ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Blood Parameters ◽  
Infectious Bursal Disease ◽  
Post Inoculation ◽  
Significant Difference ◽  
Bursal Disease ◽  
Group A ◽  
Group B

Abstract The study investigated the mitigating effects of two probiotics on blood parameters of ISA Brown chicks inoculated with a very virulent infectious bursal disease virus (vvIBDV). Two hundred chicks were assigned into four groups of 50 birds each. Groups A and B were administered Antox® in water and Bactofort® in feed daily from 1 to 42 days of age and inoculated with a vvIBDV at 28 days and C and D served as positive and negative controls, respectively. Blood samples were examined for changes in packed cell volume (PCV), haemoglobin concentration (Hb), red blood cell (RBC), total white blood cell (TWBC), heterophil and lymphocyte counts seven days post inoculation. The PCV between groups A and C differed (P < 0.05) and in group B it was higher (P < 0.05) than that of group C. The Hb concentration between groups A, B and C differed (P < 0.05). There was a difference (P < 0.05) in RBC counts between groups A, B, C. Differences in TWBC between group A and C were significant (P < 0.05) and TWBC in group B was higher (P < 0.05) than that of group C. There was a significant difference in heterophil (P < 0.05) and lymphocyte (P < 0.05) count between group A and C, and B and C. Heterophil/lymphocyte ratio was significantly higher in positive control compared to groups A, B, C. Antox® and Bactofort® mitigated the deleterious effects of vvIBDV on blood parameters and can assist in the control of IBD.

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