scholarly journals Effect of different post-harvest treatments on free amino acid content in Fucus vesiculosus

2020 ◽  
Author(s):  
Ekaterina Obluchinskaya
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Dry Matter ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Air Drying ◽  
Long Term Storage ◽  
Term Storage

The effect of different post-harvest treatments (fresh, freezing and air-drying) and storage duration on free amino acid (FAA) content of brown seaweed Fucus vesiculosus were studied. The FAA profile of thalli algae was studied every 3 months over 1 year. The FAA were extracted in ethanol/water (70: 30) solution, purified by ion exchange chromatography, derivatized and determined using hight-performance liquid chromatography. Free amino acid profile was significantly (p<0.001) different among the fresh, freezing and air-drying seaweeds. Long-term storage of seaweeds for 12 months in both dried and frozen form contributed to increased levels of free amino acids. The total free amino acid content increased from 285.80 mg/100 g dry matter of fresh seaweeds to 597.38 mg/100 g dry matter of dried seaweeds and to 473.84 mg/100 g dry matter of the frozen form. The total content of essential amino acids increased from 49.65 mg/100 g dry matter in fresh seaweeds to 130.45 mg/100 g dry matter of dried seaweeds and to 106.70 mg/100 g dry matter of frozen seaweeds. The content of glutamic acid responsible for the taste increased from 132.36 mg/100 g dry matter of fresh seaweeds to 169.50 mg/100 g dry matter of dried seaweeds, and to 242.00 mg/100 g dry matter of frozen seaweeds. Thus, the long-term storage of dried and frozen seaweeds contributes to the improvement of their qualities.

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

scholarly journals Free amino acid content in muscle tissue of bighead carp and wels catfish

2016 ◽  
Vol 72 (10) ◽  
pp. 632-636 ◽  
Author(s):  
Renata Pyz-Łukasik ◽  
Maria Szpetnar ◽  
Waldemar Paszkiewicz ◽  
Marcin R. Tatara ◽  
Adam Brodzki
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Muscle Tissue ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Bighead Carp ◽  
Predatory Fish ◽  
Free Amino Acid Content ◽  
Natural Diet

The present research was aimed at a comparative analysis of free amino acid (FAA) levels in the muscle tissue of herbivorous and predatory fish. The FAA concentration in the muscle tissue samples from bighead carp and wels catfish was determined by ion-exchange chromatography. The bighead carp muscle tissue, as compared to that of wels catfish, showed significantly higher concentrations of arginine, histidine, methionine, phenyloalanine, alanine, asparagine, serine, glycine, and taurine, but significantly lower levels of isoleucine, leucine, threonine, valine, glutamine, cystationine, β-alanine, ethanoloamine, as well as aspartic, glutamic, cysteic, α- and γ-aminobutyric acids. The muscle tissues of bighead carp and wels catfish did not differ significantly in the levels of cystine, lysine, tryptophan, tyrosine, α-aminoadipic acid, cytrulline, ornithine and 1-methyl-histidine. Proline was detected only in the wels catfish muscle. The results obtained have shown differences in free amino acid concentration in muscle tissue of examined fish. A differentiated natural diet induces changes in free amino acid content in fish tissues. Knowledge of levels of free amino acids which are precursors for biogenic amines facilitates setting the safety criteria for fish and fishery products from species other than those mentioned in the Commission Regulation No 2073/2005

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