Genetic Diversity of Black and Aromatic Rice Cultivar (Oryza sativa L.) from Various Regions in Indonesia using Random Amplified Polymorphic DNA Markers (RAPD)

Author(s):  
Ummi Sholikhah ◽  
Parjanto ◽  
Tri Handoyo ◽  
Ahmad Yunus
2015 ◽  
Vol 63 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Masako Okoshi ◽  
Koya Matsuno ◽  
Kazutoshi Okuno ◽  
Masami Ogawa ◽  
Tomio Itani ◽  
...  

Tropics ◽  
2007 ◽  
Vol 16 (4) ◽  
pp. 343-355 ◽  
Author(s):  
Nguyen Loc HIEN ◽  
Wakil Ahmad SARHADI ◽  
Yosei OIKAWA ◽  
Yutaka HIRATA

2016 ◽  
Vol 22 (4) ◽  
pp. 547-555 ◽  
Author(s):  
Humaira Ashraf ◽  
Amjad M. Husaini ◽  
M. Ashraf Bhat ◽  
GA Parray ◽  
Salim Khan ◽  
...  

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
T. Rekha ◽  
Kottackal Poulose Martin ◽  
V. B. Sreekumar ◽  
Joseph Madassery

Random amplified polymorphic DNA fingerprinting was performed to assess the genetic diversity among rarely cultivated traditional indica rice (Oryza sativa L.) varieties collected from a tribal hamlet of Kerala State, India. A total of 664 DNA bands amplified by 15 primers exhibited 72.9% polymorphism (an average of 32.3 polymorphic bands per primer). The varieties Jeerakasala and Kalladiyaran exhibited the highest percent (50.19%) polymorphism, while Thondi and Adukkan showed the lowest (9.85%). Adukkan (78 bands) and Jeerakasala (56 bands) yielded the highest and the lowest number of amplicons, respectively. Unweighted Pair Group Method with Arithmetic mean analysis using the Dice similarity coefficient showed the highest value of similarity coefficient between the varieties Adukkan and Thondi, both shared higher level of similarity (0.81), followed by Kanali and Thondi (0.88). Of the three subclusters, the varieties of Adukkan, Thondi, Kanali, Mannuveliyan, Thonnuranthondi, and Chennellu grouped together with a similarity of 0.77. The second group represented by Navara, Gandhakasala, and Jeerakasala with a similarity coefficient of 0.76 formed a cohesive group. The variety Kalladiyaran formed an isolated position that joined the second cluster. The Principal Coordinate Analysis also showed separation of Kalladiyaran from the other varieties.


2019 ◽  
Vol 98 (3) ◽  
Author(s):  
Anurag Mishra ◽  
Pushpendra Kumar ◽  
Md. Shamim ◽  
Kapil K. Tiwari ◽  
Parveen Fatima ◽  
...  

Author(s):  
Mehfuz Hasan ◽  
Mohammad Sharif Raihan

Genetic polymorphism and relationships among 30 commercial varieties of Bangladeshi aromatic rice (Oryza sativa L.) were established using random amplified polymorphic DNA (RAPD) primers. Out of fifty 10-mer RAPD primers screened initially, four were chosen and used in a comparative analysis of different varieties of indigenous Bangladeshi aromatic rice. Of the 33 total RAPD fragments amplified, 7 (21.21%) were found to be shared by individuals of all eight varieties. The remaining 26 fragments were found to be polymorphic (78.79%). Pair-wise estimates of similarity ranged from 0.101 to 0.911. Highest genetic diversity was determined between Radhunipagol and Dubsail varieties (0.911). The amount of genetic diversity within aromatic rice germplasm was quite high as determined by the genetic similarity coefficients between varieties. Genetic similarities obtained from RAPD data were also used to create a cluster diagram. Cluster analysis using an un-weighted pair-group method with arithmetic averages (UPGMA) was used to group the varieties and the 30 aromatic rice varieties were grouped into 6 clusters where cluster I includes the maximum number of varieties (9). Cluster VI includes minimum number of varieties (2). This Study offered a rapid and reliable method for the estimation of variability between different varieties which could be utilized by the breeders for further improvement of the local aromatic rice varieties.


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