An Improved Microscopic Method for The Rapid Diagnosis of Emerging Microsporidian Parasite, Enterocytozoon hepatopenaei in Shrimp Farms

2018 ◽  
Vol 115 (4) ◽  
pp. 758 ◽  
Author(s):  
P. Ezhil Praveena ◽  
T. Bhuvaneswari ◽  
A. Navaneeth Krishnan ◽  
V. Jagadeesan ◽  
J. Joseph Sahaya Rajan ◽  
...  
Keyword(s):  
Rapid Diagnosis ◽  
Microsporidian Parasite ◽  
Microscopic Method ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms

scholarly journals Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 233 ◽  
Author(s):  
Patharapol Piamsomboon ◽  
Seong-Kyoon Choi ◽  
Bambang Hanggono ◽  
Yani Lestari Nuraini ◽  
Fatma Wati ◽  
...  
Keyword(s):  
Latin America ◽  
Taqman Probe ◽  
Contaminated Water ◽  
Asian Countries ◽  
Microsporidian Parasite ◽  
Copy Numbers ◽  
Live Feeds ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms ◽  
Normal Shrimp

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

scholarly journals White spot syndrome virus and Enterocytozoon hepatopenaei in shrimp farms in Costa Rica

2020 ◽  
pp. 479-489
Author(s):  
Nelson Peña Navarro ◽  
Ruth Castro Vásquez ◽  
Gaby Dolz
Keyword(s):  
Costa Rica ◽  
White Spot Syndrome Virus ◽  
Low Frequency ◽  
Small Subunit ◽  
White Spot ◽  
Production Cycle ◽  
Central Pacific ◽  
White Spot Syndrome ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms

Introduction. The white spot syndrome virus (WSSV) causes systemic destruction of ectodermal and mesodermal tissues in shrimp and can cause 100 % mortality. Enterocytozoon hepatopenaei (EHP) is an intracellular microsporidian that causes lysis of epithelial cells in hepatopancreatic tubules in shrimp. Objective. Detect the presence of WSSV and EHP in fifteen shrimp farms in the Gulf of Nicoya and the Central Pacific from Costa Rica. Materials and methods. Between January 2017 and July 2018 water during the filling process of the pond, postlarvae, and juvenile shrimp of Penaeus vannamei was collected, during a production cycle in each one of the fifteen farms. Samples were analyzed through polymerase chain reaction (PCR) using primers and protocols to detect the VP41B gene in WSSV, and the small subunit ribosomal RNA gene of EHP. Results. The presence of WSSV was detected in one farm, while EHP was not detected in any of the fifteen farms. The sequencing of the amplified segments of the VP41B gene showed 100 % identity with isolated shrimp sequences in Taiwan and Mexico. Conclusion. WSSV was detected at a very low frequency and EHP was not detected in this study. It is necessary to continue monitoring these agents in the country’s shrimp farms.

Prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in Jiangsu Province, China

2019 ◽  
Vol 27 (3) ◽  
pp. 675-683 ◽  
Author(s):  
Hui Shen ◽  
Yi Qiao ◽  
Xihe Wan ◽  
Ge Jiang ◽  
Xianping Fan ◽  
...  
Keyword(s):  
Jiangsu Province ◽  
Microsporidian Parasite ◽  
Enterocytozoon Hepatopenaei

scholarly journals Histopathology and Molecular Characterization of Microsporidian Parasites in Cultured Shrimp Penaeus Monodon Fabricius 1798 of Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Md Munjur Hossain ◽  
Nusrat Jahan Punom ◽  
Md Mostavi Enan Eshik ◽  
Mst Khadiza Begum ◽  
Md Aminul Islam Bhuiyan ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Pcr Amplification ◽  
Histological Section ◽  
Rrna Gene ◽  
Microsporidian Parasite ◽  
Specific Primers ◽  
West Region ◽  
Field Samples ◽  
Enterocytozoon Hepatopenaei

Black tiger shrimp (Penaeus monodon Fabricius 1798) cultured in Bangladesh was investigated for the presence of microsporidian parasite. Histological section of hepatopancreas showed a large number of microsporidian spores under light microscopy. Spores under scanning electron microscope appeared oval shapes. Histology of infected shrimps showed severe degeneration of hepatopancreatic tubules. Early and late stage of microsporidian parasites in hepatopancreatic tubules were also observed. DNA extracted from the hepatopancreas of shrimps were subjected to PCR amplification using primers targeting microsporidian SSU rRNA gene. The PCR amplified an expected product of ~328 bp and the sequences showed 81 - 82% identity with the Paranucleospora theridion reported from western Norway in 2008. Further screening of field samples was carried out using EHP-specific primers. DNA extracted from ten hepatopancreas samples of P. monodon were tested and none found to be positive for EHP (Enterocytozoon hepatopenaei). This is the first report for the identification of microsporidian parasites in cultured shrimp along the south-west region of Bangladesh. Asiat. Soc. Bangladesh, Sci. 45(2): 187-196, December 2019

scholarly journals A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0166320 ◽  
Author(s):  
Pattana Jaroenlak ◽  
Piyachat Sanguanrut ◽  
Bryony A. P. Williams ◽  
Grant D. Stentiford ◽  
Timothy W. Flegel ◽  
...  
Keyword(s):  
Nested Pcr ◽  
False Positive ◽  
Environmental Samples ◽  
Pcr Assay ◽  
False Positive Detection ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms

Quick report on prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in India

2016 ◽  
Vol 48 (7) ◽  
pp. 3980-3984 ◽  
Author(s):  
Karthikeyan Kesavan ◽  
Ravi Mani ◽  
Itami Toshiaki ◽  
Raja Sudhakaran
Keyword(s):  
Microsporidian Parasite ◽  
Enterocytozoon Hepatopenaei

scholarly journals Clinical Investigation and Molecular Diagnosis of Microsporidian Enterocytozoon hepatopenaei (EHP) in Shrimps from Selangor, Malaysia

2021 ◽  
Vol 5 (2) ◽  
pp. 67-75
Author(s):  
Shamala Marimuthu ◽  
Chandramathi Samudi Raju ◽  
Subha Bhassu
Keyword(s):  
Growth Retardation ◽  
Clinical Investigation ◽  
Preliminary Investigation ◽  
Environmental Parameters ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Microsporidian Parasite ◽  
Minimum Spanning Network ◽  
Positive Rate ◽  
Enterocytozoon Hepatopenaei

In recent years, shrimp aquaculture production in Southeast Asia countries was highly infected by, microsporidian parasite, EHP. Recently, shrimps from farms located in Selangor were encountering growth retardation and it highly concerned us to carry out a clinical investigation in both farms (Location A and B). EHP infected P. monodon samples were collected from both locations. A total of 43 shrimp samples were collected and diagnosed via PCR using the 18S rRNA gene. Environmental parameters were found relatively higher than the advised values. It indicates that the farms are highly infected and toxic which had led to growth retardation and mortality. However, no significant differences were observed (except water temperatures) between the locations. The phylogenetic analysis expressed EHP detected from Malaysia formed in one clade and all the reference sequences clustered based on geographical continents (Asian-Latin America). Minimum-spanning network analysis shows that Malaysia samples Location A are genetically related with other Asian EHP samples even though the total positive rate of EHP infection was lower. However, samples from Location B were not clustered within the EHP clade and shows the highest genetic variance within Malaysia and among Asian samples. This preliminary investigation results confirmed the incidence of EHP outbreak in Malaysia.

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