Quick report on prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in India

2016 ◽  
Vol 48 (7) ◽  
pp. 3980-3984 ◽  
Author(s):  
Karthikeyan Kesavan ◽  
Ravi Mani ◽  
Itami Toshiaki ◽  
Raja Sudhakaran
Keyword(s):  
Microsporidian Parasite ◽  
Enterocytozoon Hepatopenaei

scholarly journals Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 233 ◽  
Author(s):  
Patharapol Piamsomboon ◽  
Seong-Kyoon Choi ◽  
Bambang Hanggono ◽  
Yani Lestari Nuraini ◽  
Fatma Wati ◽  
...  
Keyword(s):  
Latin America ◽  
Taqman Probe ◽  
Contaminated Water ◽  
Asian Countries ◽  
Microsporidian Parasite ◽  
Copy Numbers ◽  
Live Feeds ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms ◽  
Normal Shrimp

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

Prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in Jiangsu Province, China

2019 ◽  
Vol 27 (3) ◽  
pp. 675-683 ◽  
Author(s):  
Hui Shen ◽  
Yi Qiao ◽  
Xihe Wan ◽  
Ge Jiang ◽  
Xianping Fan ◽  
...  
Keyword(s):  
Jiangsu Province ◽  
Microsporidian Parasite ◽  
Enterocytozoon Hepatopenaei

scholarly journals Histopathology and Molecular Characterization of Microsporidian Parasites in Cultured Shrimp Penaeus Monodon Fabricius 1798 of Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Md Munjur Hossain ◽  
Nusrat Jahan Punom ◽  
Md Mostavi Enan Eshik ◽  
Mst Khadiza Begum ◽  
Md Aminul Islam Bhuiyan ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Pcr Amplification ◽  
Histological Section ◽  
Rrna Gene ◽  
Microsporidian Parasite ◽  
Specific Primers ◽  
West Region ◽  
Field Samples ◽  
Enterocytozoon Hepatopenaei

Black tiger shrimp (Penaeus monodon Fabricius 1798) cultured in Bangladesh was investigated for the presence of microsporidian parasite. Histological section of hepatopancreas showed a large number of microsporidian spores under light microscopy. Spores under scanning electron microscope appeared oval shapes. Histology of infected shrimps showed severe degeneration of hepatopancreatic tubules. Early and late stage of microsporidian parasites in hepatopancreatic tubules were also observed. DNA extracted from the hepatopancreas of shrimps were subjected to PCR amplification using primers targeting microsporidian SSU rRNA gene. The PCR amplified an expected product of ~328 bp and the sequences showed 81 - 82% identity with the Paranucleospora theridion reported from western Norway in 2008. Further screening of field samples was carried out using EHP-specific primers. DNA extracted from ten hepatopancreas samples of P. monodon were tested and none found to be positive for EHP (Enterocytozoon hepatopenaei). This is the first report for the identification of microsporidian parasites in cultured shrimp along the south-west region of Bangladesh. Asiat. Soc. Bangladesh, Sci. 45(2): 187-196, December 2019

An Improved Microscopic Method for The Rapid Diagnosis of Emerging Microsporidian Parasite, Enterocytozoon hepatopenaei in Shrimp Farms

2018 ◽  
Vol 115 (4) ◽  
pp. 758 ◽  
Author(s):  
P. Ezhil Praveena ◽  
T. Bhuvaneswari ◽  
A. Navaneeth Krishnan ◽  
V. Jagadeesan ◽  
J. Joseph Sahaya Rajan ◽  
...  
Keyword(s):  
Rapid Diagnosis ◽  
Microsporidian Parasite ◽  
Microscopic Method ◽  
Enterocytozoon Hepatopenaei ◽  
Shrimp Farms

scholarly journals Clinical Investigation and Molecular Diagnosis of Microsporidian Enterocytozoon hepatopenaei (EHP) in Shrimps from Selangor, Malaysia

2021 ◽  
Vol 5 (2) ◽  
pp. 67-75
Author(s):  
Shamala Marimuthu ◽  
Chandramathi Samudi Raju ◽  
Subha Bhassu
Keyword(s):  
Growth Retardation ◽  
Clinical Investigation ◽  
Preliminary Investigation ◽  
Environmental Parameters ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Microsporidian Parasite ◽  
Minimum Spanning Network ◽  
Positive Rate ◽  
Enterocytozoon Hepatopenaei

In recent years, shrimp aquaculture production in Southeast Asia countries was highly infected by, microsporidian parasite, EHP. Recently, shrimps from farms located in Selangor were encountering growth retardation and it highly concerned us to carry out a clinical investigation in both farms (Location A and B). EHP infected P. monodon samples were collected from both locations. A total of 43 shrimp samples were collected and diagnosed via PCR using the 18S rRNA gene. Environmental parameters were found relatively higher than the advised values. It indicates that the farms are highly infected and toxic which had led to growth retardation and mortality. However, no significant differences were observed (except water temperatures) between the locations. The phylogenetic analysis expressed EHP detected from Malaysia formed in one clade and all the reference sequences clustered based on geographical continents (Asian-Latin America). Minimum-spanning network analysis shows that Malaysia samples Location A are genetically related with other Asian EHP samples even though the total positive rate of EHP infection was lower. However, samples from Location B were not clustered within the EHP clade and shows the highest genetic variance within Malaysia and among Asian samples. This preliminary investigation results confirmed the incidence of EHP outbreak in Malaysia.

scholarly journals First Report of Enterocytozoon hepatopenaei Infection in Pacific Whiteleg Shrimp (Litopenaeus vannamei) Cultured in Korea

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3150
Author(s):  
Bo-Seong Kim ◽  
Gwang-Il Jang ◽  
Su-Mi Kim ◽  
Young-Sook Kim ◽  
Yu-Gyeong Jeon ◽  
...  
Keyword(s):  
Growth Retardation ◽  
Litopenaeus Vannamei ◽  
Inflammatory Responses ◽  
Microsporidian Parasite ◽  
First Report ◽  
South Korean ◽  
Control And Prevention ◽  
Whiteleg Shrimp ◽  
Enterocytozoon Hepatopenaei ◽  
Important Disease

The consumption of cultured crustaceans has been steadily increasing, and Pacific whiteleg shrimp (Litopenaeus vannamei) are major cultivated invertebrates worldwide. However, shrimp productivity faces a variety of challenges, mainly related to outbreaks of lethal or growth retardation-related diseases. In particular, hepatopancreatic microsporidiosis caused by the microsporidian parasite Enterocytozoon hepatopenaei (EHP) is an important disease associated with growth retardation in shrimp. Here, we report the detection of EHP through histopathological, molecular and electron microscopy methods in the hepatopancreas of Pacific whiteleg shrimp with growth disorder in a South Korean farm. Phylogenetic analysis showed a clade distinct from the previously reported EHP strains isolated in Thailand, India, China and Vietnam. An EHP infection was not associated with inflammatory responses such as hemocyte infiltration. Although EHP infection has been reported worldwide, this is the first report in the shrimp aquaculture in Korea. Therefore, an EHP infection should be managed and monitored regularly for effective disease control and prevention.

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