Evaluation of Serum Alkaline Phosphatase and Acid Phosphatase in Relation to Abo Blood Groups and Genotypes of Male Blood Donors in University College Hospital Ibadan, Oyo State

OBJECTIVE: To find out the distribution of ABO and Rh (D) blood groups among blood donors in districtMardan and to help transfusion services in the area.STUDY DESIGN: Cross sectional descriptive study.MATERIALS AND METHODS: The study was conducted in DHQ Hospital Mardan from 1st January2012 to 31st December 2012. A total of 2893 healthy adult, blood donors (both volunteer and directed) fromdistrict Mardan were included. Two ml of blood anti-coagulated with EDTA was taken and both ABO andRh (D) blood groups were determined using commercially available anti-sera of Biolaboratories. Thedistribution ofABO and Rh blood groups in the local community were then calculated.RESULTS: Among ABO blood groups “ B” (27.97%) was the most prevalent, followed by “0” (27.93%),“ A” (24.75%) and “ AB” (19.36%). Group Rh positive (94.30%) was more prevalent than Rh negative(5.70%). Similarly 0+ve (26.65%) was most frequent followed by B+ve (26.17%), A+ve (23.16%), AB+ve(18.32%), B've(1.80%),Ave(l.59%),O ve( 1.28%) and ABve( 1.04%).CONCLUSION: Blood group 'B' was most frequent among the ABO blood groups and Rh (D) positiveamong Rh blood groups while0+vewas the most frequent blood group in the study population.KEYWORDS: ABO Blood grouping, Rh (D) blood grouping, Mardan.

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Prevalence of ABO blood groups in patients with haematological malignancies: a retrospective study

Hematology & Transfusion International Journal ◽

10.15406/htij.2020.08.00215 ◽

2020 ◽

Vol 8 (1) ◽

pp. 13-17

Author(s):

Ashwin Mathew ◽

Kirana Pailoor, ◽

Konchady Deepthi ◽

Suresh Shenoy

Keyword(s):

Sex Ratio ◽

Blood Group ◽

Haematological Malignancy ◽

Geographical Location ◽

Blood Groups ◽

Blood Type ◽

Haematological Malignancies ◽

Abo Blood Groups ◽

College Hospital ◽

And Gender

Background: The pattern and distribution of Haematological malignancies vary depending on age, sex and geographical location. Studies on the association between Haematological cancers and ABO blood types have been largely conflicting. The objective of our study was to determine the distribution of ABO blood groups among patients with Haematological malignancies and to correlate with age and gender. Materials and Methods: This was a chart based observational descriptive study involving the review of records of 85 patients with various Haematological malignancies in Father Muller Medical College Hospital for a period of two years from July 2017- June 2019. Age, gender, malignant condition, ABO and Rh blood group were collected for each case. Data was analyzed statistically by frequency and percentage. Results: The peak age incidence for various Haematological malignancies was in the fifth and sixth decades of life with a mean age of 47.The male to female sex ratio was almost 1:1. Majority of the patients were of the O blood group and an association of O blood group with various hematological malignancies with male preponderance was noted. Altogether, Leukemias were the most common Haematological malignancy observed in this study. Conclusion: The distribution, age and sex ratio of Haematological malignancies in our study was comparable to those reported by other authors with Acute Myeloid Leukemia (AML) being the most common Haematological malignancy in our environment. There was no significant association between Haematological cancers and ABO blood type of the patients, but probably individuals with O Rh positive blood group are more prone to

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Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia

Malaria Research and Treatment ◽

10.1155/2016/8043768 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Getaneh Alemu ◽

Mohammedaman Mama

Keyword(s):

Blood Group ◽

Malaria Infection ◽

Blood Donors ◽

Blood Groups ◽

High Rate ◽

Abo Blood Group ◽

Asymptomatic Malaria ◽

Abo Blood Groups ◽

Group Frequency ◽

Rh Blood Group

Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22±0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P=0.022). High rate of parasitemia was seen in blood group O donors (6.899, P=0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

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Fluorometric Assay of Serum Acid or Alkaline Phosphatase, Either in Solution or on a Semisolid Surface

Clinical Chemistry ◽

10.1093/clinchem/21.12.1791 ◽

1975 ◽

Vol 21 (12) ◽

pp. 1791-1794 ◽

Cited By ~ 8

Author(s):

Bernd Rietz ◽

George G Guilbault

Keyword(s):

Alkaline Phosphatase ◽

Enzyme Activity ◽

Acid Phosphatase ◽

Clinical Laboratory ◽

Serum Alkaline Phosphatase ◽

Buffer Solution ◽

Citrate Buffer ◽

Enzymatic Action ◽

Stable Substrate ◽

Serum Acid Phosphatase

Abstract We describe enzymatic fluorometric methods for determining activities of serum alkaline phosphatase and of serum acid phosphatase in solution and on silicone rubber pads. 4-Methylumbelliferone phosphate is used as substrate, in either tris(hydroxymethyl)aminomethane or citrate buffer. In solution, the reaction is measured at 37 °C in a 3-ml Pyrex cuvette. Measurements on the pads are also made at 37 °C, after establishing a stable substrate film by lyophilizing all reagents on the surface of the pads. Only 20 to 30 µl of substrate solution, 50 µl of buffer solution, and 1 to 10 µl of blood are necessary, making a total volume of 51 to 60 µl for each assay. The rate of appearance of the fluorescent 4-methylumbelliferone liberated from 4-methylumbelliferone phosphate by the enzymatic action is measured and equated to enzyme activity. Calibration plots of the change in fluorescence per minute vs. enzyme activity for measurements in solution and on pads show a good proportionality in the range of 30.8 to 633 U/liter for alkaline phosphatase and in the range of 0.265 to 5.3 King— Armstrong units for acid phosphatase, indicating the usefulness of these methods in the clinical laboratory.

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