scholarly journals Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes

Aging ◽  
2020 ◽  
Vol 12 (17) ◽  
pp. 17503-17527
Author(s):  
Yuhong Pan ◽  
Anchun Cheng ◽  
Xingcui Zhang ◽  
Mingshu Wang ◽  
Shun Chen ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dynamic Response ◽  
Transcriptome Analysis ◽  
Duck Tembusu Virus ◽  
Duck Embryo ◽  
Tembusu Virus ◽  
Embryo Fibroblasts

scholarly journals Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

2018 ◽  
Vol 19 (8) ◽  
pp. 2328 ◽  
Author(s):  
Guanliu Yu ◽  
Yun Lin ◽  
Yi Tang ◽  
Youxiang Diao
Keyword(s):  
Signaling Pathway ◽  
Cell Lineage ◽  
Enrichment Analysis ◽  
Receptor Signaling ◽  
Duck Tembusu Virus ◽  
Duck Embryo ◽  
Chemokine Signaling ◽  
Receptor Signaling Pathway ◽  
Tembusu Virus ◽  
Embryo Fibroblasts

Duck is a major waterfowl species in China, providing high-economic benefit with a population of up to 20–30 billion per year. Ducks are commonly affected by severe diseases, including egg-drop syndrome caused by duck Tembusu virus (DTMUV). The immune mechanisms against DTMUV invasion and infection remain poorly understood. In this study, duck embryo fibroblasts (DEFs) were infected with DTMUV and harvested at 12 and 24 h post-infection (hpi), and their genomes were sequenced. In total, 911 (764 upregulated and 147 downregulated genes) and 3008 (1791 upregulated and 1217 downregulated) differentially expressed genes (DEGs) were identified at 12 and 24 hpi, respectively. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that DEGs were considerably enriched in immune-relevant pathways, including Toll-like receptor signaling pathway, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Chemokine signaling pathway, NOD-like receptor signaling pathway, and Hematopoietic cell lineage at both time points. The key DEGs in immune system included those of the cytokines (IFN α2, IL-6, IL-8L, IL-12B, CCR7, CCL19, and CCL20), transcription factors or signaling molecules (IRF7, NF-κB, STAT1, TMEM173, and TNFAIP3), pattern recognition receptors (RIG-I and MDA5), and antigen-presenting proteins (CD44 and CD70). This suggests DTMUV infection induces strong proinflammatory/antiviral effects with enormous production of cytokines. However, these cytokines could not protect DEFs against viral attack. Our data revealed valuable transcriptional information regarding DTMUV-infected DEFs, thereby broadening our understanding of the immune response against DTMUV infection; this information might contribute in developing strategies for controlling the prevalence of DTMUV infection.

scholarly journals Duck Tembusu Virus Infection Promotes the Expression of Duck Interferon-Induced Protein 35 to Counteract RIG-I Antiviral Signaling in Duck Embryo Fibroblasts

2021 ◽  
Vol 12 ◽  
Author(s):  
Peng Zhou ◽  
Lei Ma ◽  
Zaixiao Rao ◽  
Yaqian Li ◽  
Huijun Zheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Egg Production ◽  
Isotope Labeling ◽  
Specific Interaction ◽  
Innate Immune ◽  
Duck Tembusu Virus ◽  
Duck Embryo ◽  
Tembusu Virus ◽  
Embryo Fibroblasts

Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused a substantial drop in egg production and severe neurological disorders in domestic waterfowl. Several studies have revealed that viral proteins encoded by DTMUV antagonize host IFN-mediated antiviral responses to facilitate virus replication. However, the role of host gene expression regulated by DTMUV in innate immune evasion remains largely unknown. Here, we utilized a stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics analysis of DTMUV-infected duck embryo fibroblasts (DEFs) to comprehensively investigate host proteins involved in DTMUV replication and innate immune response. A total of 250 differentially expressed proteins were identified from 2697 quantified cellular proteins, among which duck interferon-induced protein 35 (duIFI35) was dramatically up-regulated due to DTMUV infection in DEFs. Next, we demonstrated that duIFI35 expression promoted DTMUV replication and impaired Sendai virus-induced IFN-β production. Moreover, duIFI35 was able to impede duck RIG-I (duRIG-I)-induced IFN-β promoter activity, rather than IFN-β transcription mediated by MDA5, MAVS, TBK1, IKKϵ, and IRF7. Importantly, we found that because of the specific interaction with duIFI35, the capacity of duRIG-I to recognize double-stranded RNA was significantly impaired, resulting in the decline of duRIG-I-induced IFN-β production. Taken together, our data revealed that duIFI35 expression stimulated by DTMUV infection disrupted duRIG-I-mediated host antiviral response, elucidating a distinct function of duIFI35 from human IFI35, by which DTMUV escapes host innate immune response, and providing information for the design of antiviral drug.

Characterization of monoclonal antibodies against duck Tembusu virus E protein: an antigen-capture ELISA for the detection of Tembusu virus infection

2015 ◽  
Vol 160 (3) ◽  
pp. 757-764 ◽  
Author(s):  
Xiaofei Bai ◽  
Wulin Shaozhou ◽  
Qingshan Zhang ◽  
Chenxi Li ◽  
Na Qiu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Virus Infection ◽  
Capture Elisa ◽  
Antigen Capture Elisa ◽  
E Protein ◽  
Duck Tembusu Virus ◽  
Antigen Capture ◽  
Tembusu Virus

Generation of duck Tembusu virus using a simple reverse genetic system in duck embryo fibroblast cells

2021 ◽  
pp. 114385
Author(s):  
Feng Hu ◽  
Tong Zhu ◽  
Xiaozhen Guo ◽  
Kexiang Yu ◽  
Xiuli Ma ◽  
...  
Keyword(s):  
Genetic System ◽  
Reverse Genetic System ◽  
Fibroblast Cells ◽  
Reverse Genetic ◽  
Duck Embryo Fibroblast ◽  
Duck Tembusu Virus ◽  
Duck Embryo ◽  
Tembusu Virus

scholarly journals Corrigendum: Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in Vivo

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhiqiang Hu ◽  
Yuhong Pan ◽  
Anchun Cheng ◽  
Xingcui Zhang ◽  
Mingshu Wang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Therapeutic Target ◽  
Potential Therapeutic Target ◽  
Duck Tembusu Virus ◽  
Tembusu Virus

Response to “A comment on ‘Serological evidence of duck Tembusu virus infection in free‐grazing ducks, Thailand’”

2019 ◽  
Vol 66 (2) ◽  
pp. 1098-1099
Author(s):  
Wikanda Tunterak ◽  
Duangduean Prakairungnamthip ◽  
Patchareeporn Ninvilai ◽  
Supanat Bunyapisitsopa ◽  
Kanisak Oraveerakul ◽  
...  
Keyword(s):  
Virus Infection ◽  
Serological Evidence ◽  
Duck Tembusu Virus ◽  
Free Grazing ◽  
Tembusu Virus

scholarly journals Autophagy Is a Potential Therapeutic Target Against Duck Tembusu Virus Infection in vivo

2020 ◽  
Vol 10 ◽  
Author(s):  
Zhiqiang Hu ◽  
Yuhong Pan ◽  
Anchun Cheng ◽  
Xingcui Zhang ◽  
Mingshu Wang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Therapeutic Target ◽  
Potential Therapeutic Target ◽  
Duck Tembusu Virus ◽  
Tembusu Virus

scholarly journals Innate immune responses to duck Tembusu virus infection

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Ning Li ◽  
Jun Zhao ◽  
Yudong Yang ◽  
Yongqing Zeng ◽  
Sidang Liu
Keyword(s):  
Virus Infection ◽  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Duck Tembusu Virus ◽  
Tembusu Virus

Serological evidence of duck Tembusu virus infection in free-grazing ducks, Thailand

2018 ◽  
Vol 65 (6) ◽  
pp. 1943-1950 ◽  
Author(s):  
Wikanda Tunterak ◽  
Duangduean Prakairungnamthip ◽  
Patchareeporn Ninvilai ◽  
Supanat Bunyapisitsopa ◽  
Kanisak Oraveerakul ◽  
...  
Keyword(s):  
Virus Infection ◽  
Serological Evidence ◽  
Duck Tembusu Virus ◽  
Free Grazing ◽  
Tembusu Virus

A comment on “Serological evidence of duck Tembusu virus infection in free‐grazing ducks, Thailand”

2019 ◽  
Vol 66 (2) ◽  
pp. 1097-1097
Author(s):  
L.‐F. Zhao ◽  
J.‐L. Yang ◽  
F.‐M. Miao ◽  
T. Chen ◽  
J.‐H. Zhao
Keyword(s):  
Virus Infection ◽  
Serological Evidence ◽  
Duck Tembusu Virus ◽  
Free Grazing ◽  
Tembusu Virus
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