scholarly journals Inhibition of the RUNX1-CBFβ transcription factor complex compromises mammary epithelial cell identity: a phenotype potentially stabilized by mitotic gene bookmarking

Oncotarget ◽  
2020 ◽  
Vol 11 (26) ◽  
pp. 2512-2530
Author(s):  
Joshua T. Rose ◽  
Eliana Moskovitz ◽  
Joseph R. Boyd ◽  
Jonathan A. Gordon ◽  
Nicole A. Bouffard ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Cell Identity ◽  
Gene Bookmarking ◽  
Transcription Factor Complex

scholarly journals Glucocorticoid Receptor Activation Signals through Forkhead Transcription Factor 3a in Breast Cancer Cells

2006 ◽  
Vol 20 (10) ◽  
pp. 2304-2314 ◽  
Author(s):  
Wei Wu ◽  
Min Zou ◽  
Deanna R. Brickley ◽  
Travis Pew ◽  
Suzanne D. Conzen
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Transcription Factor ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Breast Cancer Cells ◽  
Receptor Activation ◽  
Mammary Epithelial ◽  
Forkhead Transcription Factor ◽  
Igfbp 3

Abstract Activation of the glucocorticoid receptor (GR) plays a critical role in the stress response of virtually all cell types. Despite recent advances in large-scale genomic and proteomic data acquisition, identification of physiologically relevant molecular events downstream of nuclear hormone receptor activation remains challenging. By analyzing gene expression changes 30 min after dexamethasone (Dex) treatment, we previously found that immediate induction of serum and glucocorticoid-regulated kinase-1 (SGK-1) expression is required for GR-mediated mammary epithelial cell survival signaling. We now report that activation of the GR mediates Forkhead transcription factor 3a (FOXO3a) phosphorylation and inactivation in mammary epithelial cells. GR-mediated induction of SGK-1 expression is required for FOXO3a inactivation; additional growth factor stimulation is not required. To further explore the gene expression changes that occur downstream of GR-mediated FOXO3a inactivation, we analyzed temporal gene expression data and selected GR-down-regulated genes containing core FOXO3a binding motifs in their proximal promoters. This approach revealed several previously unrecognized transcriptional target genes of FOXO3a, including IGF binding protein-3 (IGFBP-3). Endogenous IGFBP-3 expression was confirmed to be dependent on the GR-SGK-1-FOXO3a signaling pathway. Moreover, GR activation decreased FOXO3a-induced apoptosis in SK-BR-3 breast cancer cells. Collectively, our data suggest that GR-mediated FOXO3a inactivation is an important mechanism contributing to glucocorticoid-mediated mammary epithelial cell survival.

scholarly journals Integrated Single-Cell Transcriptomics and Chromatin Accessibility Analysis Reveals Regulators of Mammary Epithelial Cell Identity

Cell Reports ◽  
2020 ◽  
Vol 33 (3) ◽  
pp. 108273
Author(s):  
Nicholas Pervolarakis ◽  
Quy H. Nguyen ◽  
Justice Williams ◽  
Yanwen Gong ◽  
Guadalupe Gutierrez ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Single Cell ◽  
Mammary Epithelial Cell ◽  
Chromatin Accessibility ◽  
Mammary Epithelial ◽  
Cell Identity ◽  
Accessibility Analysis

scholarly journals Integrated Single-Cell Transcriptomics and Chromatin Accessibility Analysis Reveals Novel Regulators of Mammary Epithelial Cell Identity

2019 ◽  
Author(s):  
Nicholas Pervolarakis ◽  
Peng Sun ◽  
Guadalupe Gutierrez ◽  
Quy H. Nguyen ◽  
Darisha Jhutty ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Single Cell ◽  
Mammary Epithelial Cell ◽  
Chromatin Accessibility ◽  
Mammary Epithelial ◽  
Cell Identity ◽  
Accessibility Analysis

Role of p53 Mammary Epithelial Cell Senescence

2005 ◽  
Author(s):  
Goberdhan P. Dimri
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cell Senescence ◽  
Mammary Epithelial
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