scholarly journals Dose-dependent effect of recombinant CHH-B1 on osmoregulatory capacity and Na+/K+-ATPase expression in bilaterally eyestalk-ablated shrimp Litopenaeus vannamei

Biotecnia ◽  
2021 ◽  
Vol 23 (3) ◽  
Author(s):  
Elizabeth Ponce-Rivas ◽  
Laura Camacho-Jiménez ◽  
Fernando Diaz ◽  
Edna Sánchez-Castrejón ◽  
Ariana Montiel-Arzate

Osmoregulation in crustaceans is under neuroendocrine control by the crustacean hyperglycemic hormones (CHHs), which modify water and ion concentrations in diverse species. Previous studies done by our group suggested that CHH variant B1 (CHH-B1) has effects on the osmoregulatory responses of the Pacific white shrimp Litopenaeus vannamei. For a better understanding of the molecular action mechanisms of CHH-B1 in osmoregulation, in this work, we established the dose-dependent effect of recombinant CHH-B1 on the gene expression of Na+/K+-ATPase (NKA) in gills, in contrast to changes in the osmoregulatory capacity (OC) of bilaterally eyestalk-ablated shrimp under hyper-osmotic conditions. The results indicate that CHH-B1 regulates the OC of shrimp during hypo-regulation by modulating Na+/K+-ATPase at a transcriptional level. Our results suggest that CHH has a direct participation in the control of osmo-ionic regulation mechanisms, not only in L. vannamei but in crustaceans in general.

2011 ◽  
Vol 43 (5) ◽  
pp. 745-755 ◽  
Author(s):  
Xiang-he Liu ◽  
Ji-dan Ye ◽  
Kun Wang ◽  
Jiang-hong Kong ◽  
Wei Yang ◽  
...  

Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 5-12
Author(s):  
Alejandro Mcdonal-Vera ◽  
Wilfrido M. Contreras-Sánchez ◽  
Neil J. Duncan ◽  
Kevin Fitzsimmons ◽  
Maria J. Contreras- García ◽  
...  

In the present investigation, we evaluated if the presence of tilapia affects growth and survival of shrimp during pre-growth and grow-out phases. In both experiments, we used a random-blocks design in a single earth pond (0.75 ha) that was prepared and divided into nine (7 x 30 m) enclosures that were considered to be three blocks of three enclosures each. Each randomly stocked block consisted on Pacific white shrimp Litopenaeus vannamei (30 shrimp/m2) and three different densities (0.0, 0.25 and 0.50 fish/m2) of red tilapia hybrids (Oreochromis mossambicus x Oreochromis niloticus) to give three different treatments with three replicates. The results from the pre-growth and grow-out experiments followed the same trend. In both cases, we found no significant statistical differences (P>0.05) in the final average weight of the shrimp. However, the experimental blocks had a significant (P<0.05) effect on the mean sample weight of shrimp. Shrimp in blocks B and C were significantly bigger than shrimp from block A (P<0.05). For both experiments, we found no effect of the presence of tilapias on survival or growth of shrimp.RESUMENEvaluamos si la presencia de tilapias libres afecta el crecimiento y la supervivencia de camarones en fase de preengorda y engorda. Para ambos experimentos, utilizamos un diseño de bloques al azar en un estanque rústico (0.75 ha) que fue preparado y dividido en nueve encierros (7 x 30 m), se formaron tres bloques con tres encierros cada uno. Cada encierro dentro de cada bloque fue aleatoriamente seleccionado para sembrar camarón blanco del Pacífico, Litopenaeus vannamei (30 camarones/m2) y tres diferentes densidades (0.0, 0.25 y 0.50 peces/m2) de híbrido de tilapia roja (Oreochromis mossambicus x Oreochromis niloticus) para formar tres tratamientos con tres réplicas. Los resultados del experimento de pre-engorda y engorda siguieron la misma tendencia. En ambos experimentos no encontramos diferencias estadísticamente en el crecimiento de los camarones ni en las tilapias. Sin embargo, los bloques experimentales presentaron diferencias significativas (P<0.05) en el peso promedio final. Los camarones de los bloques B y C fueron estadísticamente más grandes (P<0.05) que los del bloque A. Para las dos etapas de crecimiento de los camarones, no se observó un efecto de la presencia de tilapias sobre la sobrevivencia o crecimiento de los camarones.


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