Association of polymorphism at intron 2 of FABP3 Gene with milk production traits in Sahiwal and Karan Fries cattle

2018 ◽  
Author(s):  
Alok Kumar Yadav ◽  
Anupama Mukherjee ◽  
Suchit Kumar
Keyword(s):  
Milk Production ◽  
Rflp Analysis ◽  
Production Traits ◽  
Milk Production Traits ◽  
Pcr Rflp ◽  
Sahiwal Cattle ◽  
Pcr Products ◽  
The Mean ◽  
Intron 2 ◽  
Karan Fries

PCR-RFLP analysis of PCR products were carried out using Aci I / SSi I for 100 Sahiwal and 115 Karan Fries cattle. In Sahiwal cattle and Karan Fries cattle, 438bp has three genotypes AA (438), AB (438+299+139 bp) and BB (299+139 bp). In Sahiwal cattle these genotypes are highly significant for FL305DPY but in Karan Fries cattle these genotypes are highly significant for FL305DMY, FLTMY, FL305DFY and FL305DPY. In Sahiwal cattle, mean ± SE of AA genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY, FL305DPY were 1809.90 ± 15.7 kg, 2029.4 ± 15.6 kg, 99.90 ± 0.66 kg, 154.87 ± 0.17 kg and 44.81 ± 0.06 kg, respectively and for AB genotype were 1800.76 ± 9.48 kg, 1993.99 ± 9.42 kg, 100.54 ± 0.39 kg, 154.79 ± 0.10 kg, 43.99 ± 0.04 kg, respectively and for BB genotype were 1830.0 ± 14.10 kg, 2032.80 ± 14.0 kg, 100.24 ± 0.59 kg, 155.11 ± 0.15 kg, 42.98 ± 0.05 kg, respectively. Heterozygous AB genotype was found to be superior for, FL305DFY trait. AA genotype was significantly superior for FL305DPY traits whereas BB genotype was found to be superior for FL305DMY, FLTMY, FL305DSNFY. In Karan Fries cattle, the mean ± SE of AA genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY, FL305DPY were 3442.17 ± 8.39 kg, 4461.93 ± 8.39 kg, 124.96 ± 7.20 kg, 277.35 ± 0.08 kg and 112.51 ± 0.08 kg, respectively and for AB genotype were 3572.69 ± 5.93 kg, 4592.45 ± 5.93 kg, 140.17 ± 5.09 kg, 278.60 ± 0.06 kg, 113.91 ± 0.05 kg, respectively and for BB genotypes were 3502.41 ± 9.19 kg, 4522.17 ± 9.19 kg, 136.91 ± 7.89 kg, 277.93 ± 0.09 kg, 113.19 ± 0.08 kg, respectively.

Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians

2000 ◽  
Vol 51 (4) ◽  
pp. 515 ◽  
Author(s):  
M. R. Shariflou ◽  
C. Moran ◽  
F. W. Nicholas
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Bovine Growth Hormone ◽  
Data Sets ◽  
Production Traits ◽  
Milk Production Traits ◽  
Maximum Benefit ◽  
Pcr Products ◽  
Trait Locus

The occurrence of the Leu127/Val127 variants of the bovine growth hormone (bGH) gene and their effect on milk production traits was investigated in Australian Holstein-Friesian cattle. Animals were genotyped for the Leu127/Val127 variants, with RFLP methodology, using PCR and AluI digestion of PCR products (AluI-RFLP). Alleles Leu127 and Val127 occurred with frequencies of 82% and 18%, respectively. The quantitative effect of this polymorphic site on milk-production traits was estimated from lactation data and test-day data. Results from the 2 data sets consistently showed that the Leu127 allele is associated with higher production of milk, fat, and protein and is dominant to Val127. The average effects of the gene substitution are 95 L for milk yield, 7 kg for fat yield, and 3 kg for protein yield per lactation. This locus may be directly responsible for quantitative variation or it may be a marker for a closely linked quantitative trait locus (QTL) for milk-production traits in Australian dairy cattle. In either case, it will be useful as an aid to selection for improvement of milk production traits. As the Leu127 allele is dominant, selection of AI sires homozygous for the Leu127 allele (Leu127/Leu127) will result in maximum benefit without the need for genotyping cows.

Prospects for genetic improvement of milk production traits of Sahiwal cattle in Kenya

2007 ◽  
Vol 2007 ◽  
pp. 69-69
Author(s):  
E.D. Ilatsia ◽  
T. K. Muasya ◽  
W. B. Muhuyi ◽  
A. K. Kahi
Keyword(s):  
Animal Model ◽  
Milk Production ◽  
Genetic Parameters ◽  
Genetic Relationships ◽  
Additive Genetic Variance ◽  
Parameter Estimates ◽  
Production Traits ◽  
Milk Production Traits ◽  
Phenotypic Parameter ◽  
Sahiwal Cattle

The primary emphasis of the long-term Sahiwal cattle breeding programme is to increase milk yield by selecting cows based on their performance in first three lactations. It is therefore important to have knowledge on the extend of additive genetic variance and genetic parameters for these traits. Genetic and phenotypic parameter estimates normally apply directly to the specific population and environment from which the data were collected. In the Sahiwal cattle in Kenya, very little is known about the genetic variation of milk production traits and their genetic relationships. Furthermore, genetic and phenotypic parameter estimates for the Sahiwal cattle based on multivariate animal model are scarce. This paper presents estimates of variance components and genetic parameters for milk production traits using trivariate animal model.

scholarly journals Associations between the bovine PPARGC1A gene and milk production traits

2010 ◽  
Vol 55 (No. 5) ◽  
pp. 195-199 ◽  
Author(s):  
I. Kowalewska-Łuczak ◽  
H. Kulig ◽  
M. Kmieć
Keyword(s):  
Protein Content ◽  
Milk Production ◽  
Common Haplotype ◽  
Production Traits ◽  
Milk Production Traits ◽  
Milk Traits ◽  
Pcr Rflp ◽  
The Individual ◽  
Jersey Cows

The aim of this study was to investigate associations between PPARGC1A genotypes and haplotypes and milk production traits. The study included 181 Jersey cows. The genotypes were identified by the PCR-RFLP method. The frequencies of the most common alleles were as follows: T – 0.63 (c.1892T>C) and A – 0.88 (c.3359A>C). The frequency of the most common haplotype was TC/AA – 0.558. The results showed that there were no statistically significant associations between the individual genotypes of both SNPs and milk traits; however, individuals with the CC/AC haplotype produced significantly (P ≤ 0.05; P ≤ 0.001) less milk and had a significantly (P ≤ 0.001) higher protein content in milk.

Identification of Genetic Variants in ABCG2 Gene Influencing Milk Production Traits in Dairy Cattle

2019 ◽  
Author(s):  
Arun Pratap Singh ◽  
A K Chakravarty ◽  
M A Mir ◽  
Ashwani Arya ◽  
Manvendra Singh
Keyword(s):  
Milk Yield ◽  
Rflp Analysis ◽  
Milk Composition ◽  
Breeding Value ◽  
Production Traits ◽  
Crossbred Cattle ◽  
Fat Percentage ◽  
Pcr Rflp ◽  
Karan Fries ◽  
Abcg2 Gene

This study was performed to investigate the polymorphisms in the ABCG2 (ATP-binding cassette sub-family G member 2) gene and to reveal the association of genotypes with breeding value (BV) for first lactation milk yield and milk composition traits of Karan Fries (HF crossbred) cattle. The traits were adjusted against the significant effect of non-genetic factors. PCR-RFLP analysis of ABCG2 (exon 14) gene revealed three genotypes. AA genotype in ABCG2 gene had significant effect on BV for average test day fat percentage. The identified potential genetic marker could be used for the development of Marker Assisted Selection (MAS) strategy for higher milk yield and milk composition traits in Karan Fries Cattle.

Effect of DGAT1 and TG gene polymorphisms on intramuscular fat and on milk production traits in different cattle breeds in Hungary

2008 ◽  
Vol 56 (2) ◽  
pp. 181-186 ◽  
Author(s):  
István Anton ◽  
Katalin Kovács ◽  
László Fésüs ◽  
József Várhegyi ◽  
László Lehel ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Longissimus Dorsi ◽  
Production Traits ◽  
Milk Production Traits ◽  
Fat Percentage ◽  
Longissimus Dorsi Muscle ◽  
Cattle Breeds ◽  
Pcr Rflp ◽  
The Difference

The objective of this study was to estimate the effect of the thyroglobulin (TG) locus on beef quality traits in some beef cattle breeds and to investigate the effect of the DGAT1 locus on milk production traits in the Hungarian Holstein Friesian population. TG and DGAT1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At the TG locus TT bulls showed the highest fat percentage values in the longissimus dorsi muscle (m. longissimus dorsi); the difference between CC and TT genotypes was significant. DGAT1 GC/GC cows had the highest milk, fat and protein yield values. Due to the relatively small number of GC/GC cows the difference proved to be significant only between AA/AA and AA/GC genotypes.

Comparative performance of F1 Friesian × Zebu heifers in Ethiopia

1983 ◽  
Vol 37 (2) ◽  
pp. 247-252 ◽  
Author(s):  
M. Alberro
Keyword(s):  
Milk Production ◽  
Addis Ababa ◽  
Disease Incidence ◽  
Production Traits ◽  
Milk Production Traits ◽  
Comparative Performance ◽  
Parasitic Resistance ◽  
The Mean ◽  
Behavioural Factors ◽  
Zebu Cows

ABSTRACTThe performance of 200 F1 Friesian ♂ × zebu ♀ and 90 zebu heifers on 14 dairy cooperative farms near Addis Ababa was compared over a 2-year period. The farms varied in management and feeding but in general the standards were low. Each farm had zebu and Friesian × zebu cows given the same management and feeding. Measurements were made of milk production traits of economic importance, including physiological and behavioural factors important for management purposes.The Friesian ♂ × zebu ♀ crossbred heifers showed an advantage in having an earlier age at puberty and at first calving, higher milk production, longer lactations, better disposition at milking and better reproductive performance. The calves from these crossbred heifers were larger at birth but there were more problems at calving. The zebu heifers withstood better the two periods of drought that occurred during the study. No significant differences were found in disease incidence, parasitic resistance, mastitis incidence or mortality. The interval between oestrus was normal in the F1 heifers but longer in the zebus. The duration of gestation was much longer in the zebus (mean 291 days). The duration of oestrus was 4·5 h for the zebu and 10·0 h for the F1 heifers, the latter being-much longer than the mean of 7·4 h reported in the literature. The F1 heifers showed the first oestrus after calving much sooner than did the zebus.

scholarly journals Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

2002 ◽  
Vol 45 (5) ◽  
pp. 421-428 ◽  
Author(s):  
A. Dybus
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
White Cattle

Abstract. Associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production traits of Black-and-White cattle with different proportional share of Holstein Friesian genes were analysed. A total of 1086 cows were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles were as follows: 0.653 for LL genotype, 0.324 for LV and 0.023 for VV, and 0.815 for GHL and 0.185 for GHV. Associations between Leu/Val polymorphism and milk production traits of cows were found only in first lactation. Cows with LL genotype had higher milk, fat and protein yield compared to LV individuals (P ≤ 0.01).

scholarly journals Evaluation of associations of the polymorphism in the placentaspecific promoter 1.1 of the <i>CYP19</i> gene in Black-and-White and Jersey cattle with milk production traits (short communication)

2006 ◽  
Vol 49 (4) ◽  
pp. 311-314 ◽  
Author(s):  
M. Jędrzejczak ◽  
I. Szatkowska ◽  
S. Zych ◽  
W. Grzesiak ◽  
E. Czerniawska-Piątkowska ◽  
...  
Keyword(s):  
Milk Production ◽  
Short Communication ◽  
Production Traits ◽  
Milk Production Traits ◽  
P450 Gene ◽  
Black And White ◽  
Jersey Cattle ◽  
Pcr Rflp ◽  
Pvuii Polymorphism ◽  
The Relationship

Abstract. The relationship between the SNP of the cytochrome P450 gene (CYP19-PvuII) and milk production traits of Black-and-White and Jersey cattle were analysed. A total of 437 cows were included in the study. A PCR-RFLP was used to genotype. The frequencies of genotypes and alleles for the Black-and-White cows were as follows: 0.8985 – AA, 0.0977 – AB, 0.0038 – BB, and 0.9474 – CYP19A, 0.0526 – CYP19B. In the Jersey, all cows were genotyped as CYP19AA (no polymorphism). There weren’t any associations between CYP19-PvuII polymorphism and milk production traits of the investigated cows.

scholarly journals <i>GHRH/Hae</i>III gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle (short communication)

2006 ◽  
Vol 49 (5) ◽  
pp. 434-438 ◽  
Author(s):  
A. Dybus ◽  
W. Grzesiak
Keyword(s):  
Gene Polymorphism ◽  
Milk Production ◽  
Short Communication ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Pcr Rflp ◽  
White Cattle ◽  
The Relationship

Abstract. The objective of this paper was to evaluate the relationship between the polymorphism of the GHRH and milk production traits of Polish Black-and-White. A total of 881 cows were included in the study. A PCR-RFLP method was used to genotyping. The frequencies of the genotypes and alleles were as follows: 0.0545 for AA, 0.3133 for AB and 0.6322 for BB, and 0.2111 for GHRHA and 0.7889 – GHRHB. There were no significant associations between GHRH/HaeIII polymorphism and milk production traits of the analysed cows.

scholarly journals Association of genetic variants of bovine <i>prolactin</i> with milk production traits of Black-and-White and Jersey cattle

2005 ◽  
Vol 48 (2) ◽  
pp. 149-156 ◽  
Author(s):  
A. Dybus ◽  
W. Grzesiak ◽  
H. Kamieniecki ◽  
I. Szatkowska ◽  
Z. Sobek ◽  
...  
Keyword(s):  
Milk Production ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Jersey Cattle ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Rsai Polymorphism ◽  
White Cattle ◽  
Jersey Cows

Abstract. Associations between polymorphism localised in the third exon of the prolactin gene (PRL-RsaI) and milk xproduction traits of Black-and-White and Jersey cattle were analysed. A total of 427 cows were included in the study. PCR-RFLP method was used. The frequencies of genotypes and alleles were as follows: 0.7107 – AA, 0.2851 – AB, 0.0042 – BB; 0.8533 – PRLA and 0.1467 – PRLB for Black-and-White cattle and 0.0919 – AA, 0.4324 – AB, 0.4757 – BB; 0.3081 – PRLA and 0.6919 – PRLB for Jersey cattle. Statistically significant differences between the breeds were observed in the frequencies of genotypes and alleles. Associations between PRL-RsaI polymorphism and milk production traits of Jersey cows and lack of associations with these traits in Black-and-White cows were observed.

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