scholarly journals ISSR marker based diversity assessment of Piper spp. in Bay Islands, India 

2018 ◽  
Author(s):  
K. Abirami ◽  
V. Baskaran ◽  
P. Simhachalam
Keyword(s):  
Genetic Diversity ◽  
Germplasm Collection ◽  
Inter Simple Sequence Repeat ◽  
Rapid Identification ◽  
The Other ◽  
Issr Marker ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Diversity Assessment ◽  
Bay Islands

Inter-simple sequence repeat (ISSR) analysis was used to study the genetic diversity of Piper spp. with its relative genus Peperomia pellucida. Among the 38 ISSR primers used, 10 primers generated 1083 bands, of which seven (80.8%) were polymorphic. The maximum polymorphism were obtained from the primers UBC 881, UBC 889, UBC 848 and UBC 830. Cluster analysis grouped the species studied into two major clusters. One cluster comprised the different accessions of Piper nigrum and is distinct from the other species of Piperaceae. The other cluster grouped the four species of Piperaceae namely Piper longum, Piper sarmentosum, Piper betle and Peperomia pellucida. The variability studies also revealed that the ISSR marker could group the accessions within the same species based on their similar geographical origin. Assessment of genetic diversity among the different species of the family Piperaceae through ISSR marker would help in rapid identification of polymorphism, assist in future germplasm collection, conservation and domestication programmes. 

scholarly journals Molecular characterization of Asplenium nidus L. – A potential ornamental fern species from Andaman

2019 ◽  
Vol 38 (4) ◽  
Author(s):  
K. Abirami ◽  
V. Baskaran ◽  
P. Simhachalam ◽  
K. Venkatesan ◽  
D.R. Singh
Keyword(s):  
Polymorphic Information Content ◽  
Germplasm Collection ◽  
Inter Simple Sequence Repeat ◽  
Rapid Identification ◽  
Issr Marker ◽  
Fern Species ◽  
South Andaman ◽  
Asplenium Nidus ◽  
Geographical Locations

Asplenium nidus L. is one of the most important terrestrial fern in Andaman and Nicobar Islands with great ornamental value. Inter simple sequence repeat (ISSR) analysis was used to study the genetic diversity of the species A. nidus L. collected from different geographical locations of the Island. Among 15 ISSR primers screened, 9 primers produced amplification and produced a total of 222 reproducible amplified bands. The number of amplified bands varied from 10 in ISSR 11 to 44 in UBC 842 with an average of 24.7 bands per primer. The range of polymorphic bands and percent polymorphism observed were 1 to 5 and 33.3 to 83.3, respectively. The polymorphic information content value of ISSR marker ranged from 0.365 to 0.485. Cluster analysis grouped the twelve accessions into three clusters (Cluster I, II and III) at Jaccard’s similarity coefficient value of 0.69 on basis of geographical locations of the accessions. The cluster I was further divided into two sub-clusters Ia and Ib at the coefficient value of 0.77 and it includes the accessions belonging to South Andaman, one each accession collected from two different locations, Little Andaman and Rangat (Middle Andaman) were grouped separately as clusters II and III, respectively. The genetic variability studies with ISSR marker grouped the accessions of A. nidus L.on basis of their geographical locations and it would help in rapid identification of polymorphism and assist in germplasm collection, conservation and domestication programmes.

Genetic diversity assessment and gene expression analysis of prolonged shelf-life genes in Mangalore melon (Cucumis melo ssp. agrestis var. acidulus)

2021 ◽  
Author(s):  
Raghavendra Gunnaiah ◽  
Ratnakar M. Shet ◽  
Ashwini Lamani ◽  
Dattatraya H. Radhika ◽  
Rudrappa C. Jagadeesha
Keyword(s):  
Genetic Diversity ◽  
Shelf Life ◽  
Candidate Genes ◽  
Cucumis Melo ◽  
South India ◽  
Ethylene Biosynthesis ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Tree Construction ◽  
Diversity Assessment

Abstract Mangalore melon (Cucumis melo ssp. agrestis var. acidulus) is a non-dessert melon, extensively grown in the coastal districts of South India, but hardly known to the rest of the World. Immature or mature fruits of Mangalore melon are used in preparation of delicious dishes such as vegetable stew, chutneys and curries. They are appreciated for nutritional values, long shelf life and biotic stress resistance. Seventy-nine accessions of Mangalore melon were collected from five states of South India and their genetic diversity was assessed using inter simple sequence repeat (ISSR) markers. Putative candidate genes of extended shelf life in Mangalore melon were studied by quantitative reverse transcription polymerase chain reaction in comparison with cantaloupe (Cucumis melo L.). Shelf life varied from 65 days to 300 days at room temperature. Six ISSR primers amplified 142 fragments ranging from 80 bp to 2380 bp with an average of 23.66 bands per marker on a high-resolution capillary electrophoresis system. Neighbor joining phylogenetic tree construction from the ISSR allele similarity based genetic distance revealed two major clusters with 46 and 33 accessions in each cluster. Expression of fruit ripening related genes of ethylene biosynthesis (1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase) and cell wall metabolism (polygalacturonase, xyloglucan endotransglucosylase/hydrolase and expansin) in Mangalore melons was significantly lower than the cantaloupe melon at 180 days after harvest. Mangalore melon is a promising genetic resource for enhancing the shelf life of melons and the putative candidate genes are useful in enhancing shelf life of cantaloupe following validation and conformation.

Genetic diversity of Dioscorea japonica germplasm in Taiwan revealed by inter-simple sequence repeat DNA markers

2015 ◽  
Vol 14 (3) ◽  
pp. 211-218
Author(s):  
Tsai-Li Kung ◽  
Kuan-Hung Lin ◽  
Shun-Fu Lin
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Dna Markers ◽  
Simple Sequence Repeat ◽  
Germplasm Collection ◽  
Inter Simple Sequence Repeat ◽  
Northern Region ◽  
Sequence Repeat ◽  
Dioscorea Japonica ◽  
Simple Sequence

There are three native varieties of edible yams (Dioscorea japonica Thunb.) in Taiwan, but germplasm has been collected from only a few. To assess the genetic diversity of 99 accessions collected from four geographically diverse regions, inter-simple sequence repeat (ISSR) DNA markers were analysed, and 90.2% of the polymorphic markers in these accessions were found. Genetic similarity among accessions ranged from 30.8 to 74.4%, averaging 48.4%. Cluster analyses revealed four main clusters plus one outlier (Dioscorea alata L.). The varieties oldhamii and pseudojaponica were separated into different clusters and var. japonica was grouped with both varieties. Since var. japonica was found to be genetically similar to var. oldhamii and var. pseudojaponica, we suggest that var. japonica is a hybrid or intermediate variety between the two varieties. Molecular analysis of variance results indicated that variation within counties (95.94%) was predominantly greater than variation among counties (3.63%) and among regions (0.43%). Overall, gene flow (Nm= 0.970) estimated from genetic differentiation (Gst= 0.340) suggests that gene flow among regions is relatively high. The highest genetic diversity (H= 0.191) and Shannon's index (I= 0.312), and a high gene flow (Nm= 3.926) of germplasm in the northern region suggest that this region is a possible centre of dispersal and domestication of yams in Taiwan. This study provided valuable information for germplasm collection and genetic improvement.

scholarly journals Genome-wide characterization of microsatellites and genetic diversity assessment of spinach in the Chinese germplasm collection

2018 ◽  
Vol 68 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Shu-Fen Li ◽  
Bing-Xiao Wang ◽  
Yu-Jiao Guo ◽  
Chuan-Liang Deng ◽  
Wu-Jun Gao
Keyword(s):  
Genetic Diversity ◽  
Germplasm Collection ◽  
Genome Wide ◽  
Diversity Assessment ◽  
Chinese Germplasm
Sign in / Sign up

Export Citation Format

Share Document