Development of CAPS Marker Linked to H2 Locus of Fusarium Wilt Resistance in Chickpea

Background: Fusarium wilt is one of the widely distributed biotic stress of chickpea limiting its productivity worldwide. The major problem limiting the resistance breeding is screening of germplasm and breeding lines for disease resistance. To address such problems, identification of molecular marker closely linked to resistance locus is an effective strategy.Methods: The RAPD marker A07C417 closely linked H2 locus of Fusarium wilt resistance locus has been converted into SCAR and the loss of initial polymorphism was recaptured by alignment of consensus sequences from SCAR amplified locus for the identification of common motifs. Further, the linkage association of this marker with Fusarium wilt resistance locus has been reaffirmed using Fusarium wilt response of RILs phenotyped for wilt reaction using wilt sick pots and also wilt sick plots.Result: Identification of single nucleotide polymorphism at the consensus SCAR locus between susceptible and resistant cultivar, enabled development of CAPS marker through suitable restriction enzyme Aci I making it an effective codominant marker system for resistance deployment via marker assisted selection.

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Evaluation of chickpea (Cicer arietinum L.) accessions for Fusarium wilt resistance

Journal of Environmental Biology ◽

10.22438/jeb/42/1/mrn-1475 ◽

2021 ◽

Vol 42 (1) ◽

pp. 82-89

Author(s):

A.K. Srivastava ◽

◽

G.P. Dixit ◽

N.P. Singh ◽

D.R. Saxena ◽

...

Keyword(s):

Fusarium Wilt ◽

Block Design ◽

Resistance Breeding ◽

Field Evaluation ◽

Gge Biplot ◽

Wilt Resistance ◽

Breeding Lines ◽

G X E Interaction ◽

Fusarium Wilt Resistance ◽

Wilt Incidence

Aim: The present study was conducted to evaluate chickpea breeding lines and popular varieties for Fusarium wilt resistance through multi-location field evaluation in major chickpea growing states of India. Methodology: Forty-five chickpea accessions were evaluated for Fusarium wilt resistance in field sick plots at Kanpur, Junagadh, Sehore and Rahuri in India during 2016 and 2017. Each accession was planted in three replications in a randomized block design. The data was subjected to analysis of variance (ANOVA) for testing the significance of variation due to accessions, environments and their interaction. GGE biplots analysis were constructed from the first two principal components (PC1 and PC2) using 45 genotypes and 8 environments using statistical software R, versions 2.15. Results: The effects of genotype, environment and G x E interaction for wilt incidence were highly significant with maximum variation caused by G x E interaction effect (50.42%), followed by genotypic (46.92 %) and environmental effect (2.24%). GGE biplot analysis revealed that Rahuri and Junagadh locations were most discriminating locations and could differentiate the wilt resistant and susceptible chickpea accession, while Kanpur was least discriminating. Junagadh was most representative followed by Sehore and Kanpur while Rahuri was least representative of the average environment. On an average, the most severe wilt incidence was observed at Junagadh, followed by Sehore, Kanpur and Rahuri over the years. Interpretation: Elite chickpea accessions possessing high level of fusarium wilt resistant at each location can be utilized for region specific breeding.The accessions IPC 2008-11, H 2010-05, GNG 1581, JG 24, SCGP-WR 28, H 2010-01 and IPC 2008-69 exhibited stable resistance over locations. These possessed resistance against multiple races of Fusarium wilt prevailing in the country and can be utilized as donors for disease resistance breeding. Key words: Chickpea, Fusarium wilt, GGE biplot, Multi-location evaluation, Stable resistance

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Development of new DNA markers linked to the Fusarium wilt resistance locus Fom-1 in melon

Plant Breeding ◽

10.1111/j.1439-0523.2010.01800.x ◽

2011 ◽

Vol 130 (2) ◽

pp. 261-267 ◽

Cited By ~ 8

Author(s):

T. Tezuka ◽

K. Waki ◽

M. Kuzuya ◽

T. Ishikawa ◽

Y. Takatsu ◽

...

Keyword(s):

Fusarium Wilt ◽

Dna Markers ◽

Resistance Locus ◽

Wilt Resistance ◽

Fusarium Wilt Resistance

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Screening and Validation of Drought Tolerance and Fusarium Wilt Resistance in Advance Breeding Lines of Chickpea (Cicer arietinum L.)

Legume Research - An International Journal ◽

10.18805/lr-4745 ◽

2021 ◽

Author(s):

Yuvaraja Lambani ◽

Laxuman . ◽

R. Lokesha ◽

Mahendar Thudi ◽

Manish Roorkiwal ◽

...

Keyword(s):

Fusarium Wilt ◽

Agricultural Research ◽

Snp Markers ◽

Research Station ◽

Early Maturity ◽

Wilt Resistance ◽

Breeding Lines ◽

Terminal Drought ◽

Drought Tolerant ◽

Fusarium Wilt Resistance

Background: Approximately 90% of the world’s chickpea is grown under rainfed conditions where terminal drought is one of the major constraints limiting productivity. The need of short-duration, Fusarium wilt tolerant cultivars/elite lines and able to escape drought due to early maturity were required. Methods: The present investigation was carried out using 54 genotypes, generated from six diverse crosses, along with ten checks (resistant/tolerance, susceptible) were screened against drought and Fusarium wilt at Zonal Agricultural Research Station, Kalaburagi, Karnataka (Latitude: 17.36 and Longitude: 76.82) during crop season 2018-19. Result: The results revealed that higher PCV, GCV, heritability, percent genetic advance were exhibited by number of pods per plant and seed yield per plot, whereas lower PCV, GCV recorded for days to 50% flowering and days to maturity in both normal and late sown conditions. The advanced breeding lines viz., KCD-8, KCD-24, KCD-28, KCD-32, KCD-37 and KCD-53 were identified as drought tolerant lines based on drought tolerant indices (viz., MP, YSI, DTE and DSI). The lines KCD-48 and KCD-32 were identified as Fusarium wilt resistance with lowest PDI of 1.47 and 2.46 respectively, as they were screened in wilt sick plot and further these were validated and confirmed the resistant alleles using two unpublished SNP markers (FW2_30366110 and FW2_30365816). The advanced breeding lines KCD-32 and KCD-37 were identified as drought tolerant and Fusarium wilt resistant.

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Molecular Mapping of a Gene Conferring Fusarium Wilt Resistance in Lentil (Lens culinaris Medikus subsp. culinaris) using Bulked-segregant Analysis

Legume Research - An International Journal ◽

10.18805/lr-4700 ◽

2021 ◽

Author(s):

Jitendra Kumar Meena ◽

H.K. Dikshit ◽

M. Aski ◽

Soma Gupta ◽

Akanksha Singh ◽

...

Keyword(s):

Resistance Gene ◽

Fusarium Wilt ◽

Lens Culinaris ◽

Molecular Mapping ◽

Bulked Segregant Analysis ◽

Resistance Breeding ◽

Wilt Resistance ◽

Serious Disease ◽

Ssrs Markers ◽

Fusarium Wilt Resistance

Background: Vascular wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causes severe yield losses worldwide. For effective disease resistance breeding the inheritance and mapping of wilt resistance gene (s) is necessary. Therefore, the present investigation was focused on study the mode of inheritance and tag/map gene (s) for fusarium wilt resistance in lentil. Methods: Bulked segregant analysis (BSA) approach was used to identify markers that were tightly linked to Fusarium wilt resistance gene. The inheritance and mapping of wilt-resistance gene (s) in lentil was investigated in F2 and F2:3 populations derived from L9-12×ILL10965 cross, whereas L9-12 and ILL10965 were susceptible and resistant parents, respectively. Result: More than two hundreds SSRs markers were surveyed for the parental polymorphism, of which twenty nine were found polymorphic. These polymorphic SSRs were used for the bulked-segregant analysis (BSA) using both parents and its respective resistant and susceptible bulks, and three SSRs viz. PBALC233, PBALC1409 and PBALC203 could distinguish the respective bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBLAC233 was also found present on the same linkage group at a distance of 10.2 cM from PBLAC1409.

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