scholarly journals FEATURES OF EPISOTIC PROCESS AND MOLECULAR-GENETIC CHARACTERISTICS OF VIRUS ISOLATES OF RABIES IN TVER REGION

2018 ◽  
Vol 63 (3) ◽  
pp. 115-123
Author(s):  
A. M. Gulukin ◽  
A. A. Shabejkin ◽  
V. V. Makarov ◽  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Molecular Genetic ◽  
Phylogenetic Group ◽  
N Gene ◽  
Genetic Characteristics ◽  
Temporal Features ◽  
Epizootic Process ◽  
Tver Region ◽  
Virus Isolates

Rabies epidemic situation in the Tver Region has been studied. Animals of different species that had confirmed clinical rabies were statistically analyzed. It was established that the features of the course of epizootics in the Tver region correspond to the regularities characteristic of rabies of the natural-focal type. As a result of sequencing of the rabies virus N gene and phylogenetic analysis, the isolates studied were assigned to the central phylogenetic group. With the help of the geoinformatic system, nosological maps of the Tver region were obtained and the spatial- temporal features of the course of the epizootic process of rabies infection were studied.

scholarly journals MOLECULAR-GENETIC CHARACTERIZATION OF FIELD ISOLATES OF RABIES VIRUS IDENTIFIED IN THE TERRITORY OF VLADIMIR, MOSCOW, TVER, NIZHNY NOVGOROD AND RYAZAN REGIONS

2017 ◽  
Vol 62 (3) ◽  
pp. 101-108
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
A. M. Gulyukin ◽  
A. A. Shabeykin ◽  
I. V. Polyakova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Molecular Genetic ◽  
Phylogenetic Group ◽  
Molecular Genetic Study ◽  
Field Isolates ◽  
Vladimir Region ◽  
Pcr Products ◽  
Molecular Genetic Characterization

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

scholarly journals Sequencing and sequence analysis of partial nucleoprotein (N) gene and phylogenetic analysis of rabies virus field isolates from Gujarat state, India

VirusDisease ◽  
2017 ◽  
Vol 28 (3) ◽  
pp. 320-327
Author(s):  
Dhaval H. Vagheshwari ◽  
Bharat B. Bhanderi ◽  
Rafyuddin A. Mathakiya ◽  
Mayurdhvaj K. Jhala
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Rabies Virus ◽  
N Gene ◽  
Gujarat State ◽  
Field Isolates ◽  
Nucleoprotein N

scholarly journals Molecular Epidemiology of Rabies in Wild Canidae in Tunisia

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2473
Author(s):  
Zied Bouslama ◽  
Habib Kharmachi ◽  
Nourhene Basdouri ◽  
Jihen Ben Salem ◽  
Samia Ben Maiez ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Wild Animals ◽  
Genetic Characteristics ◽  
Bayesian Phylogenetic Analysis ◽  
Viral Sequencing ◽  
Virus Isolates ◽  
Virus Strains ◽  
Natural Barrier

Rabies is a viral zoonosis that is transmissible to humans via domestic and wild animals. There are two epidemiological cycles for rabies, the urban and the sylvatic cycles. In an attempt to study the epidemiological role of wild canidae in rabies transmission, the present study aimed to analyze the genetic characteristics of virus isolates and confirm prior suggestions that rabies is maintained through a dog reservoir in Tunisia. Virus strains isolated from wild canidae were subject to viral sequencing, and Bayesian phylogenetic analysis was performed using Beast2 software. Essentially, the virus strains isolated from wild canidae belonged to the Africa-1 clade, which clearly diverges from fox-related strains. Our study also demonstrated that genetic characteristics of the virus isolates were not as distinct as could be expected if a wild reservoir had already existed. On the contrary, the geographic landscape is responsible for the genetic diversity of the virus. The landscape itself could have also acted as a natural barrier to the spread of the virus.

scholarly journals Comparative molecular and genetic characterization of rabies viruses (Rabies lyssavirus, Lyssavirus, Rhabdoviridae) circulated in the Russian Federation in 1985–2016

2020 ◽  
Vol 65 (1) ◽  
pp. 41-48
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
M. A. Losich ◽  
A. L. Elakov ◽  
A. M. Gulyukin ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Rabies Virus ◽  
Russian Federation ◽  
Amino Acid Sequences ◽  
Phylogenetic Study ◽  
Human Populations ◽  
Human Beings ◽  
The Russian Federation ◽  
Virus Isolates

Introduction. Rabies caused by the neurotropic virus of the genus Lyssavirus, Rhabdoviridae family, which infects all warm-blooded vertebrates including human beings. The homology level of the amino acid sequences for Lyssaviruses nucleoprotein reaches 78–93%.Aim – study the genetic diversity and molecular epidemiology of Lyssaviruses circulated in the Russian Federation in 1985–2016.Material and methods. 54 isolates of rabies virus isolated from animals, and 2 isolates from humans, 4 vaccine strains of rabies virus: RV-97, ERA, Shchelkovo 51, ERAG333 used in phylogenetic study. Phylogenetic analysis was performed using Genbank data on genome fragments of 73 rabies virus isolates and 9 EBLV-1 isolates. DNASTAR V.3.12, Bio Edit 7.0.4.1 and MEGA v.10.0.5, Primer Premier 5 programs have been used.Results. Comparative molecular genetic analysis of genomes fragments of 130 Lissaviruses, isolated on the territory of the RF, Ukraine in 1985-2016, vaccine strains of rabies virus, showed their distribution by geographical feature. Comparison of the nucleoprotein fragments of the rabies virus isolates with vaccine strains revealed 4 marker mutations: V56I (Eurasian group), L/V95W (Central group), D101N/S/T, and N/G106D. Phylogenetic analysis of the isolate «Juli», isolated from a human bitten by a bat proved his belonging to the European Bat lyssavirus-1a.Discussion. Study of the molecular epidemiology of rabies within the Russian Federation allows for the genotyping of the viruses and helps to study the hidden mechanisms of rabies infection in animal and human populations, and to characterize vaccine strains, including during oral vaccination.Conclusion. Further study of the molecular epidemiology of rabies within the Russian Federation and the countries bordering it is important.

scholarly journals Phylogenetic Analysis of the Rabies Virus N-coding Region in Lithuanian Rabies Isolates

2009 ◽  
Vol 78 (2) ◽  
pp. 273-280
Author(s):  
Dainius Zienius ◽  
Kristina Sajute ◽  
Henrikas Žilinskas ◽  
Arunas Stankevicius
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Virus Isolate ◽  
N Gene ◽  
Bootstrap Support ◽  
Gene Sequences ◽  
Red Foxes ◽  
North East ◽  
The North ◽  
Nucleoprotein N

Rabies infection among wild and domestic animals constitutes a well-known problem in Lithuania, but only one dog rabies virus isolate sequence (1992) from Lithuania was used in the European rabies virus phylogenetic analysis. The objective of this work was to determine nucleoprotein (N) gene sequences and genetically characterize the rabies virus isolates in order to learn which virus group (biotype) is circulating in reservoir species in Lithuania. Classical rabies virus isolate nucleoprotein (N) gene sequences from different parts of Lithuania were found to be closely related to each other and demonstrated nucleotide identity from 97.7 to 100% and could be placed in one lineage with 100% bootstrap support. All 12 sequences of raccoon dogs, red foxes, dogs and marten rabies viruses exhibited 97.7 - 99.0% identity to previously published sequences from Eastern parts of Poland, Estonia, Finland, and the North-Eastern part of Russia. Phylogenetic analysis revealed that all Lithuanian strains belong to the North East Europe (NEE) group of rabies virus.

scholarly journals Genetic Mapping of Rabies Virus in Dogs as a Basis for Disease Control

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Muharam Saepulloh ◽  
R. M. Abdul Adji
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Phylogenetic Relationship ◽  
Rabies Virus ◽  
Virus Isolate ◽  
Protein Gene ◽  
Genetic Characteristics ◽  
Nucleoprotein Gene ◽  
N Protein

The purpose of this study was to determine the genetic characteristics of rabies virus based on phylogenetic relationship among rabies virus in various regions in Indonesia. The amino acid sequence of the nucleoprotein gene of rabies virus isolate from Banten (RV/Banten01/dog/2007),Makasar (RV/MKS-26/dog/2010), Bukit Tinggi (RV/BKT-52/dog/2009 and RV/BKT-58/dog/2009), Medan (RV/Medan27/dog/2007)andBali(RV/Bali-1/dog/2009;RV/Bali-2/dog/2009;RV/Bali-3/dog/2009),Indonesiawasdetermined.TheseisolatesshowedahighdegreeofhomologyamongIndonesianisolateswhichreached100%.Meanwhile,thelevelofhomologybetweenrabiesvirusisolatesfromcatsrabiesvirusisolatesfromdogsreached97%.ResultsofphylogeneticanalysisusingtheaminoacidsequencesoftheNgenesshowedthatallofIndonesianrabiesvirusisolateswerecloselyrelatedtorabiesvirusesfromChinathanthosefromThailand,Laos,Burma,andVietnamwhichgeograficallycloser to Indonesia. Data obtained from the phylogenetic analysis is expected to trace the source of rabies spread and thepossibility to create a vaccines which more suitable with rabies virus that spreads in Indonesia. Based on the phylogenetic relationship analysisusing the amino acid sequence of the rabies virus N protein gene showed that all of rabies virus isolated from Indonesian regions share a highhomology with others ranging from 97-100%..Key words: sequencing, rabies, nucleoprotein gene (N), homology

Evolutionary analysis of rabies virus using the partial Nucleoprotein and Glycoprotein gene in Mumbai region of India

2021 ◽  
Author(s):  
Rajesh Raghunanth Pharande ◽  
Sharmila Badal Majee ◽  
Satish S. Gaikwad ◽  
S. D. Moregoankar ◽  
AnilKumar Bannalikar ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Evolutionary Dynamics ◽  
Recent Common Ancestor ◽  
N Gene ◽  
Evolutionary Analysis ◽  
Coalescent Analysis ◽  
Most Recent Common Ancestor ◽  
Indian Isolates ◽  
Highest Posterior Density

Nearly 1.7 million cases of dog bites are reported every year in India and many cases of animal rabies are left unattended and undiagnosed. Therefore, a mere diagnosis of rabies is not sufficient to understand the epidemiology and the spread of the rabies virus (RV) in animals. There is a paucity of information about the evolutionary dynamics of RV in dogs and its biodiversity patterns in India. In total, 50 dog-brain samples suspected of rabies were screened by the nucleoprotein- (N) and glycoprotein- (G) gene PCR. The N and G genes were subsequently sequenced to understand the molecular evolution in these genes. The phylogenetic analysis of the N gene revealed that six isolates in the Mumbai region belonged to a single Arctic lineage. Time-scaled phylogeny by Bayesian coalescent analysis of the partial N gene revealed that the time to the most recent common ancestor (TMRCA) for the sequences belonged to the cluster from 2006.68 with a highest posterior density of 95 % betweeen 2005–2008, which is assigned to Indian lineage I. Migration pattern revealed a strong Bayes factor between Mumbai to Delhi, Panji to Hyderabad, Delhi to Chennai, and Chennai to Chandigarh. Phylogenetic analysis of the G gene revealed that the RVs circulating in the Mumbai region are divided into three lineages. Time-scaled phylogeny by the Bayesian coalescent analysis method estimated that the TMRCA for sequences under study was from 1993 and Indian clusters was from 1962. In conclusion, the phylogenetic analysis of the N gene revealed that six isolates belonged to single Arctic lineages along with other Indian isolates and they were clustered into a single lineage but divided into three clades based on the G-gene sequences. The present study highlights and enhances the current molecular epidemiology and evolution of RV and revealed strong location bias and geographical clustering within Indian isolates on the basis of N and G genes.

Phylogenetic analysis of nucleoprotein gene of dog rabies virus isolates from Southern India

2009 ◽  
Vol 9 (5) ◽  
pp. 976-982 ◽  
Author(s):  
T. Nagarajan ◽  
S.B. Nagendrakumar ◽  
B. Mohanasubramanian ◽  
S. Rajalakshmi ◽  
N.R. Hanumantha ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Southern India ◽  
Nucleoprotein Gene ◽  
Virus Isolates

scholarly journals Molecular-genetic characteristics of strain Escherichia coli serogroup O26 causing diarrheal diseases in children

2018 ◽  
Vol 18 (3) ◽  
pp. 85-90
Author(s):  
M A Makarova ◽  
A V Dmitriev ◽  
Z N Matveeva ◽  
K A Kaftyreva
Keyword(s):  
Virulence Genes ◽  
Molecular Genetic ◽  
Clinical Manifestations ◽  
Phylogenetic Group ◽  
Intestinal Infection ◽  
Genetic Characteristics ◽  
E Coli ◽  
Saint Petersburg ◽  
Antigenic Properties ◽  
Genes Encoding

Aim. To determine the serotype and virulence factors of the E. coli serogroup O26 isolated from children with diarrheal syndrome. Materials and methods. Fifty-three strains of E. coli O26 isolated in 2014-2016 from the stool of children with clinical manifestations of acute intestinal infection in Saint Petersburg were studied. Phenotypic (enzymatic and antigenic properties), molecular genetic [detection of virulence genes of enteropathogenic (EPEC - eae, bfp, hlyA), and enterohemorrhagic (EHEC - eae, stx1, stx2, ehxA), genes encoding O- and H-antigens (rfb and fliC), genes, defining phylogenetic group (chuA, yjaA и TspE)] methods were used. The phylogenetic group and the production Shiga toxins were determined. Results. All strains were identical to the antigen characteristics of serotype O26:H11 and phylogenetic group B1. Two pathogroups were created based on the set of virulence genes: a-EPEC (64.2%) and EHEC (35.8%). Strains EHEC produced Shiga-toxin 1, encoding gen stx1. No differences in enzymatic activities were found between the strains of E. coli О26:H11 for EPEC and EHEC strains. Conclusion. In the population of E. coli O26:H11, which caused acute intestinal infection in children in Saint Petersburg, more than 30% of the strains belonged to the highly virulent group EHEC. Molecular-genetic methods should be used for reliable detection of pathogens.

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