Diagnostics possibilities of erythrocytes analysis by the method of laser interference microscopy

2021 ◽  
Vol 66 (1) ◽  
pp. 22-25
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
P. S. Ignatyev ◽  
A. A. Belov ◽  
V. A. Petrov
Keyword(s):  
Functional Activity ◽  
Interference Microscopy ◽  
Qualitative Assessment ◽  
Critical Conditions ◽  
Laser Interference ◽  
Functional Capabilities ◽  
Surface Nanostructure ◽  
Laser Interference Microscopy ◽  
Microscopy Method ◽  
Altered Cell

The morphofunctional state of erythrocytes significantly changes by the onset and progression of various diseases, as well as in critical conditions, injuries, accompanied by severe tissue damage. This leads to a violation of microcirculation and has an effect on hemorheology in general. In connection with this, there is a need for the development of adequate methods for assessing the functional activity of erythrocytes. The aim of the work was to study the possibilities of laser interference microscopy in the analysis of the functional state of erythrocytes. The results allowed us to quantify the state of the phase height and phase diameter of erythrocytes. Analysis of the surface nanostructure gave a qualitative assessment of the functional activity of cells. The following patterns were revealed: the action of adrenaline causes the appearance of echinocytes with an increase in phase height and decrease phase diameter and the appearance of spicules on the surface of cells. The action of cortisol leads to a decrease in the phase height and the phase diameter with the appearance of numerous loosening of the structure of the cell surface. Moreover, the traditional method of analyzing the distribution of erythrocytes in a population by morphology (discocytes, stomatocytes, and degeneratively altered cell forms) did not reveal statistically significant differences. Thus, the results indicate that the interference microscopy method allows a qualitative assessment of the morphofunctional activity of native cells. This increases the information content of the analysis and leads to the objectification of data on the functional capabilities of erythrocytes.

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

2008 ◽  
Vol 13 (3) ◽  
pp. 034004 ◽  
Author(s):  
Alexey R. Brazhe ◽  
Nadezda A. Brazhe ◽  
Georgy V. Maksimov ◽  
Pavel S. Ignatyev ◽  
Andrey B. Rubin ◽  
...  
Keyword(s):  
Phase Modulation ◽  
Cell Imaging ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Real time laser interference microscopy for bar-spread polystyrene/poly(methyl methacrylate) blends

2014 ◽  
Vol 52 (15) ◽  
pp. 985-992 ◽  
Author(s):  
Ehtsham Ul Haq ◽  
Daniel T. W. Toolan ◽  
Jillian A. Emerson ◽  
Thomas H. Epps ◽  
Jonathan R. Howse ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Real Time ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Poly Methyl Methacrylate ◽  
Laser Interference Microscopy

Use of laser interference microscopy to study the state of UV(B)-irradiated mouse peritoneal macrophages

2012 ◽  
Vol 55 (6) ◽  
pp. 726-730 ◽  
Author(s):  
M. V. Zagubizhenko ◽  
A. I. Yusipovich ◽  
S. K. Pirutin ◽  
V. L. Minaev ◽  
Yu. B. Kudryashov
Keyword(s):  
Peritoneal Macrophages ◽  
The State ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
Mouse Peritoneal Macrophages

Assessing the Functional State of Red Blood Cells by Using the Laser Interference Microscopy

2021 ◽  
Vol 15 (4) ◽  
pp. 388-392
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
A. A. Belov ◽  
P. S. Ignatiev ◽  
V. B. Metelin
Keyword(s):  
Red Blood Cells ◽  
Functional State ◽  
Blood Cells ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

VITAL MORPHOMETY OF RBC IN THEIR INTERACTION WITH STRESS HORMONES

2020 ◽  
pp. 146-147
Author(s):  
A. Deryugina ◽  
P. Ignatiev ◽  
V. Metelin ◽  
A. Belov ◽  
V. Petrov
Keyword(s):  
Normal State ◽  
Physiological State ◽  
Stress Hormones ◽  
Interference Microscopy ◽  
Extreme Condition ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
As Stress ◽  
To Receive

The possibility to use the laser interference microscopy in order to assess the RBCs morphology in normal state and in interaction with cortisol and adrenalin as stress hormones was studied. It was established that the laser interference microscopy represented an important instrument permitting to receive the information about physiological state of the RBCs as well as of the organism in the whole in extreme condition. New aspects of cortisol and adrenalin impact on the RBC morphology are defined

Laser interference microscopy in erythrocyte study

2009 ◽  
Vol 105 (10) ◽  
pp. 102037 ◽  
Author(s):  
A. I. Yusipovich ◽  
E. Yu. Parshina ◽  
N. Yu. Brysgalova ◽  
A. R. Brazhe ◽  
N. A. Brazhe ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Study of cancer and normal cells dynamics based on laser interference microscopy

2020 ◽  
Author(s):  
Aleksander S. Nikitiuk ◽  
Anna O. Voronina ◽  
Yuliya A. Beloglazova ◽  
Olga S. Ozernykh ◽  
Victoria V. Grishko ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Normal Cells ◽  
Laser Interference Microscopy

Petrographic constraints on modelling the crystallization of basalt magma, Cow Lakes, southeast Oregon

1988 ◽  
Vol 25 (4) ◽  
pp. 486-494
Author(s):  
J. K. Russell ◽  
G. T. Nixon ◽  
T. H. Pearce
Keyword(s):  
Basaltic Magma ◽  
Olivine Basalt ◽  
Significant Proportion ◽  
Thermodynamic Modelling ◽  
Interference Microscopy ◽  
Basalt Magma ◽  
Laser Interference ◽  
Chemical Zoning ◽  
Laser Interference Microscopy ◽  
Crystallization Path

Thermodynamic calculations and models of olivine zoning profiles are used to estimate the crystallization history of a basaltic magma from Cow Lakes, southeast Oregon. The lava is an alkali olivine basalt containing olivine and plagioclase phenocrysts and microphenocrysts. The geometry and range of chemical zoning in the olivine phenocrysts have been delineated by laser interference microscopy and electron microprobe analysis. The olivine phenocrysts are characterized by homogeneous cores and rims that exhibit strong, continuous, normal zoning (ΔFo = 7–19 mol%).Thermodynamic modelling has been used to estimate the magmatic crystallization path of the Cow Lakes basalt on the basis of the phenocryst assemblage and mineral compositions. The calculated crystallization path begins at 1290 °C and 0.5 GPa ([Formula: see text]) with equilibrium crystallization of the olivine to 1265 °C. Plagioclase appears at 1225 °C, followed by clinopyroxene at 1205 °C. Intratelluric crystallization was terminated prior to crystallization of the clinopyroxene, which is seen in the groundmass but not as phenocrysts.The thermodynamic modelling provides a means to numerically simulate the zoning patterns in olivine defined by the laser interference microscopy. Simulated and observed zoning patterns both have compositionally flat cores and strongly zoned rims. The extent of zoning observed in the olivine phenocrysts is, however, approximately twice the predicted extent, and it appears that a significant proportion of olivine phenocrysts crystallized during ascent or upon eruption.

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