scholarly journals Effect of PO2 on growth and physiological characteristics of Candida utilis in multistage tower fermentor.

1986 ◽  
Vol 32 (7) ◽  
pp. 183-189
Author(s):  
J. PÁCA
1983 ◽  
Vol 48 (7) ◽  
pp. 1984-1995 ◽  
Author(s):  
Jindřich Zahradník ◽  
Mojmír Rychtera ◽  
Jan Kratochvíl ◽  
Ludmila Havlíčková ◽  
Jan Čermák

Cultivation of the yeast Candida utilis on sulphite liquors was studied in a tower fermentor with ejector-type gas distributor and additional bubble bed showering. The aim of the work was to test the fermentor applicability for the cultivation process studied and to obtain data characterizing the fermentation in the tested unit. The effect of medium composition and dilution rate on selected cultivation parameters was determined in the fermentor with active volume 0.1 m3 and the hydrodynamic and energy characteristics of the apparatus were evaluated. The experimental results proved the fermentor to be suitable for the cultivation process studied. Favourable effect of the optimized fermentation medium was observed both on the values of characteristic cultivation parameters and on the efficiency of the utilization of energy supplied to the system.


Author(s):  
E. Keyhani

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).


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