scholarly journals Genomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR

2015 ◽  
Vol 9 (4) ◽  
pp. 15-21
Author(s):  
Zahra Ebrahim ◽  
Keyvan Tadayon ◽  
Nader Mosavari ◽  
◽  
◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Vaccinal Strain ◽  
Genomic Characterization

scholarly journals Molecular characterization of Mycobacterium avium subsp. paratuberculosis (MAP) in dairy cattle of Nepal

2020 ◽  
Vol 5 (6) ◽  
pp. 212-217
Author(s):  
S. Singh ◽  
◽  
I. P. Dhakal ◽  
U. M. Singh ◽  
B. Devkota ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Molecular Characterization ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Restriction Endonuclease Analysis ◽  
Cattle Population ◽  
Control And Prevention ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Specific Sequences

Paratuberculosis caused by Mycobacterium avium subspecies paratuberculosis (MAP), is an economically important, endemic in many parts of the globe, and regarded as high prevalent disease of domestic and wild animals, especially ruminants, which is manifest as chronic granulomatous enteritis with decreased milk production, with serious cases resulting in progressive emaciation and death. Understanding the genetic variability of MAP, strains are important in diagnosis, epidemiological investigation, and therefore the formation of strategies for prevention and control of the disease. Thus, this study was designed to grasp the molecular characterization of MAP isolates of Nepal, as pioneer research of this area. Total of 46 MAP isolates obtained from cattle population of three different locations of dairy pocket areas of Chitwan, Nepal were typed using IS1311 polymerase chain reaction-restriction endonuclease analysis (PCR-REA) to research the MAP genotype of Nepal. The extracted DNA samples (n=46) were analyzed for the presence of MAP specific sequences (IS900) using PCR and DNA samples were further subjected to genotype differentiation using IS1311 PCR-REA and IS1311 L2 PCR-REA methods. All the DNA samples were positive for the entire three MAP specific sequences based PCRs. This study revealed that ‘Bison type’ strain is the single most prevalent MAP genotype circulating within the domestic cattle population of Nepal. IS1311 PCR-REA showed that MAP DNA samples of Nepal origin belonged to ‘Bison type’, whereas, IS1311 L2 PCR-REA method showed similarity with "Indian Bison type" and different restriction profiles of ‘Bison type’ genotype as compared to non-Indian strains. The study concludes that in Nepal, "Bison type" MAP stains was prevalent in all the MAP samples obtained from dairy cattle. These results have important epidemiological implications regarding control and prevention of paratuberculosis in Nepal.

Characterization of Mycobacterium avium subspecies paratuberculosis disseminated infection in dairy cattle and its association with antemortem test results

2008 ◽  
Vol 127 (3-4) ◽  
pp. 300-308 ◽  
Author(s):  
María C. Antognoli ◽  
Franklyn B. Garry ◽  
Heather L. Hirst ◽  
Jason E. Lombard ◽  
Michelle M. Dennis ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Test Results ◽  
Disseminated Infection

Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU–VNTR loci

2010 ◽  
Vol 144 (1-2) ◽  
pp. 118-126 ◽  
Author(s):  
Elena Castellanos ◽  
Beatriz Romero ◽  
Sabrina Rodríguez ◽  
Lucía de Juan ◽  
Javier Bezos ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis

Isolation and characterization of a novel bacteriophage against Mycobacterium avium subspecies paratuberculosis

2014 ◽  
Vol 159 (10) ◽  
pp. 2659-2674 ◽  
Author(s):  
Simone Basra ◽  
Hany Anany ◽  
Lioubov Brovko ◽  
Andrew M. Kropinski ◽  
Mansel W. Griffiths
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Isolation And Characterization

Identification and characterization of an immunogenic 22 kDa exported protein of Mycobacterium avium subspecies paratuberculosis

2005 ◽  
Vol 54 (11) ◽  
pp. 1083-1092 ◽  
Author(s):  
Chris Dupont ◽  
Keith Thompson ◽  
Cord Heuer ◽  
Brigitte Gicquel ◽  
Alan Murray
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Smegmatis ◽  
Signal Sequence ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Mycobacterial Species ◽  
Gene Encoding ◽  
Exported Protein ◽  
Culture Supernatants ◽  
Identification And Characterization

An exported 22 kDa putative lipoprotein was identified in an alkaline phosphatase gene fusion library of Mycobacterium avium subsp. paratuberculosis and expressed in Mycobacterium smegmatis. The full nucleic acid sequence of the gene encoding P22 was determined and the ORF was cloned into a mycobacterial expression vector, enabling full-length P22 to be produced as a C-terminal polyhistidine-tagged protein in M. smegmatis. N-terminal sequencing of the recombinant protein confirmed cleavage of a signal sequence. Native P22 was detected in culture supernatants and cell sonicates of M. avium subsp. paratuberculosis strain 316F using rabbit antibody raised to recombinant P22. Investigation of the presence of similar genes in other mycobacterial species revealed that the gene was present in Mycobacterium avium subsp. avium and similar genes existed in Mycobacterium intracellulare and Mycobacterium scrofulaceum. Database searches showed that P22 belonged to the LppX/LprAFG family of mycobacterial lipoproteins also found in Mycobacterium leprae and in members of the Mycobacterium tuberculosis complex. P22 shared less than 75 % identity to these proteins. Recombinant P22 was able to elicit interferon-gamma secretion in blood from eight of a group of nine sheep vaccinated with a live attenuated strain of M. avium subsp. paratuberculosis (strain 316F) compared to none from a group of five unvaccinated sheep. Antibody to P22 was detected by Western blot analysis in 10 out of 11 vaccinated sheep, in two out of two clinically affected cows and in 11 out of 13 subclinically infected cows.

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