Influence of Combinations of Bacteria on the Levels of Prostaglandin E2, Interleukin-1β, and Granulocyte Elastase in Gingival Crevicular Fluid and on the Severity of Periodontal Disease

2006 ◽  
Vol 77 (6) ◽  
pp. 1025-1031 ◽  
Author(s):  
Stella Airila-Månsson ◽  
Birgitta Söder ◽  
Kirsti Kari ◽  
Jukka H. Meurman
Keyword(s):  
Periodontal Disease ◽  
Prostaglandin E2 ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1Β ◽  
Granulocyte Elastase ◽  
Crevicular Fluid

scholarly journals Nutritional intervention in patients with periodontal disease: clinical, immunological and microbiological variables during 12 months

2008 ◽  
Vol 101 (6) ◽  
pp. 879-885 ◽  
Author(s):  
Axel Jenzsch ◽  
Sigrun Eick ◽  
Fausi Rassoul ◽  
Regina Purschwitz ◽  
Holger Jentsch
Keyword(s):  
Metabolic Syndrome ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Nutritional Intervention ◽  
Gingival Index ◽  
Granulocyte Elastase ◽  
The Metabolic Syndrome ◽  
Probing Depth ◽  
Crevicular Fluid

The role of nutrition in onset, progression and treatment of periodontitis has not been thoroughly evaluated. In the present prospective clinical study, we investigated the influence of a nutritional intervention on changes in clinical, microbiological and immunological periodontal variables during a period of 12 months in patients with the metabolic syndrome and chronic periodontitis. Twenty female subjects with the metabolic syndrome and mild to moderate chronic periodontitis participated in a guided nutritional intervention programme. Examinations were assessed before, and at 2 weeks, 3, 6 and 12 months after intervention. Clinical measurements included probing depth, Löe and Silness gingival index and Quigley–Hein plaque index. In gingival crevicular fluid, periodontopathogens, levels of IL-1β and IL-6 as well as the activity of granulocyte elastase were determined. In stimulated saliva, antioxidative and oxidative variables were measured. After 12 months the following significant changes could be observed: reduction of clinical probing depth (2·40 v. 2·20 mm; P < 0·001), reduction of gingival inflammation (gingival index 1·13 v. 0·9; P < 0·001), reduced concentrations of IL-1β (4·63 v. 1·10 pg/ml per site; P < 0·001) as well as IL-6 (1·85 v. 0·34 pg/ml per site; P = 0·022) in gingival crevicular fluid. Bacterial counts in gingival crevicular fluid as well as oxidative and antioxidative variables in saliva showed no significant changes. Only salivary catalase showed a tendency to lower values. These findings indicate that in patients with the metabolic syndrome wholesome nutrition might reduce inflammatory variables of periodontal disease and promote periodontal health.

scholarly journals Levels of prostaglandin E2 (PGE2) in gingival crevicular fluid from smokers and non-smokers with gingivitis and chronic periodontal disease

2015 ◽  
Vol 7 (5) ◽  
pp. 54-59 ◽  
Author(s):  
Alessandra da Cruz Galhardo Camargo Gabriela ◽  
Pereira dos Santos Marcelo ◽  
Linhares Coutinho Silva Natalia ◽  
Luisa Palhares de Miranda Ana ◽  
Luiz Mendonca Tributino Jorge
Keyword(s):  
Periodontal Disease ◽  
Prostaglandin E2 ◽  
Gingival Crevicular Fluid ◽  
Chronic Periodontal Disease ◽  
Crevicular Fluid

scholarly journals PROSTAGLANDIN LEVELS IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS PATIENT WITH HYPERTENSION

2020 ◽  
pp. 19-22
Author(s):  
RADITYA PRIHARNANTO ◽  
ROBERT LESSANG ◽  
SRI LELYATI C MASULILI ◽  
FATIMAH MARIA TADJOEDIN ◽  
HERLIS RAHDEWATI ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Periodontal Disease ◽  
Prostaglandin E2 ◽  
Gingival Crevicular Fluid ◽  
Clinical Sample ◽  
Tooth Surface ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Significant Difference ◽  
Crevicular Fluid

Objective: Periodontitis is an infection that involved tooth-supporting tissues by dental plaque biofilm on the tooth surface and host immune responseas causal to as inflammation resolution. Prostaglandin E2 (PGE2) as an inflammatory mediator has been implicated in the pathogenesis of variouschronic inflammatory diseases, including periodontitis, and as a regulator of blood pressure. This study aims to compare the levels of prostaglandinin the gingival crevicular fluid (GCF) of periodontitis patients with hypertension and non-hypertension.Methods: A total sample of 62 patients was examined and consists of 44 patients (aged 50–90 years) who were hypertension group and 18 patients(aged 30–49 years) who were non-hypertension groups as a control. Hypertension state was measured by blood pressure based on anamnesisand using sphygmomanometer mercury. The clinical sample was collected from 62 gingival crevicular fluids [GCF] of periodontal disease subject.Measurement of the clinical parameter of probing pocket depth [PD], and bleeding on probing [BOP ≥1] was included as a diagnostic requisition.The PD and clinical attachment loss [CAL] was defined as present if the PD was ≥5 mm and CAL was ≥1 mm. Prostaglandin E2 level was estimated ingingival crevicular fluid samples by using the enzyme-linked immunosorbent assay.Results: The level of PGE2 was statistically significant difference in hypertension patient compare with non-hypertension (p<0.05). There was asignificant difference in PD, recession, and CAL (p<0.05).Conclusion: Higher blood pressure might be related to the potential risk of inflammation and progression periodontal disease.

scholarly journals Correlation of Levels of Interleukin-1β in Gingival Crevicular Fluid to the Clinical Parameters of Chronic Periodontitis

2011 ◽  
Vol 12 (1) ◽  
pp. 52-59 ◽  
Author(s):  
AU Chaudhari ◽  
GN Byakod ◽  
PF Waghmare ◽  
VM Karhadkar
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Interleukin 1Β ◽  
Clinical Parameters ◽  
Tissue Destruction ◽  
Tooth Mobility ◽  
Crevicular Fluid

ABSTRACT Aim Background and objectives: IL-1β is a potent stimulator of bone resorption and has been implicated in the pathogenesis of periodontal destruction. Therefore, this study was designed to compare the levels of IL-1β of chronic periodontitis patients with the healthy subjects. Another objective of this study was to correlate IL-1β levels with the clinical parameters of the periodontal disease progression. Methods For this study, total 60 subjects were chosen (30- healthy and 30-chronic periodontitis). Simplified oral hygiene index (OHI-S), gingival index (GI), periodontal disease index (PDI), probing depth (PD), tooth mobility, bleeding on probing (BOP) were recorded for all the subject. Gingival crevicular fluid (GCF) was collected and subjected for ELISA for estimation of IL-1β Results At the periodontal diseased sites, the IL-1β levels increased at least 2-fold as compared with healthy subjects. This increase was highly significant (p = 0.0000). Within the test group, IL-1β levels correlated positively and significantly with PDI, PD, BOP and tooth mobility. The correlations of IL-1β with PD (p = 0.000) and IL-1β with BOP (p = 0.0004) were highly significant. Interpretation and conclusion These data suggest that amount of GCF IL-1β is closely associated with periodontal status. This relationship may be valuable in monitoring periodontal disease activity. Clinical significance It could be stated from this study on IL- 1β that there seem to be a strong correlation between periodontal tissue destruction and IL-1β. Furthermore IL-1β level could also differentiate between active and inactive periodontal lesions. How to cite this article Chaudhari AU, Byakod GN, Waghmare PF, Karhadkar VM. Correlation of Levels of Interleukin-1β in Gingival Crevicular Fluid to the Clinical Parameters of Chronic Periodontitis. J Contemp Dent Pract 2011;12(1):52-59.

scholarly journals Effect of ovarian activity on orthodontic tooth movement and gingival crevicular fluid levels of interleukin-1β and prostaglandin E2 in cats

2012 ◽  
Vol 83 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Ahmet Arif Celebi ◽  
Serhat Demirer ◽  
Bulent Catalbas ◽  
Sevket Arikan
Keyword(s):  
Prostaglandin E2 ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Interleukin 1Β ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Activity ◽  
Coil Spring ◽  
Significant Difference ◽  
Crevicular Fluid

Abstract Objective: To evaluate whether there is any correlation between ovarian activity and two potent bone-resorbing mediators (prostaglandin E2 [PGE2], interleukin-1β [IL-1β]) secreted from the gingival crevicular fluid (GCF) during orthodontic tooth movement. Materials and Methods: Eighteen female cats were included in this study. Animals were randomly divided into three groups (estrous, anestrous, and ovariectomized groups), each having six queens. Estrous was induced by administration of 150 IU equine chorionic gonadotropin (eCG) to queens of the estrous group. A closed-coil spring, applied with 80 g of tipping force to the canine, was attached between the maxillary canine and mini-implant. GCF was collected on days 0, 6, and 12 from each cat to examine PGE2 and IL-1β during orthodontic tooth movement in cats. The PGE2 and IL-1β levels were determined with enzyme-linked immunosorbent assay. Results: There was no significant difference (P &gt; .05) between anestrous and the ovariectomized groups in terms of tooth movement on days 6 and 12 of distalization. In contrast, tooth movement in the estrous group was lower (P &lt; .05) than in the remaining two groups (anestrous and ovariectomized). The mean PGE2 and IL-1β levels of the canine teeth of the estrous groups were significantly lower than the remaining two groups on days 6 and 12 (P &lt; .05) of coil spring applications. Conclusions: These results indicate that ovarian activity can affect orthodontic tooth movement and GCF levels of IL-1β and PGE2 in cats.

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