scholarly journals Nutritional intervention in patients with periodontal disease: clinical, immunological and microbiological variables during 12 months

2008 ◽  
Vol 101 (6) ◽  
pp. 879-885 ◽  
Author(s):  
Axel Jenzsch ◽  
Sigrun Eick ◽  
Fausi Rassoul ◽  
Regina Purschwitz ◽  
Holger Jentsch
Keyword(s):  
Metabolic Syndrome ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Nutritional Intervention ◽  
Gingival Index ◽  
Granulocyte Elastase ◽  
The Metabolic Syndrome ◽  
Probing Depth ◽  
Crevicular Fluid

The role of nutrition in onset, progression and treatment of periodontitis has not been thoroughly evaluated. In the present prospective clinical study, we investigated the influence of a nutritional intervention on changes in clinical, microbiological and immunological periodontal variables during a period of 12 months in patients with the metabolic syndrome and chronic periodontitis. Twenty female subjects with the metabolic syndrome and mild to moderate chronic periodontitis participated in a guided nutritional intervention programme. Examinations were assessed before, and at 2 weeks, 3, 6 and 12 months after intervention. Clinical measurements included probing depth, Löe and Silness gingival index and Quigley–Hein plaque index. In gingival crevicular fluid, periodontopathogens, levels of IL-1β and IL-6 as well as the activity of granulocyte elastase were determined. In stimulated saliva, antioxidative and oxidative variables were measured. After 12 months the following significant changes could be observed: reduction of clinical probing depth (2·40 v. 2·20 mm; P < 0·001), reduction of gingival inflammation (gingival index 1·13 v. 0·9; P < 0·001), reduced concentrations of IL-1β (4·63 v. 1·10 pg/ml per site; P < 0·001) as well as IL-6 (1·85 v. 0·34 pg/ml per site; P = 0·022) in gingival crevicular fluid. Bacterial counts in gingival crevicular fluid as well as oxidative and antioxidative variables in saliva showed no significant changes. Only salivary catalase showed a tendency to lower values. These findings indicate that in patients with the metabolic syndrome wholesome nutrition might reduce inflammatory variables of periodontal disease and promote periodontal health.

scholarly journals Effect of Alox-15 Polymorphism on GCF Levels of Lipoxin-A4 in Chronic Periodontitis: A Preliminary Study

2017 ◽  
Vol 28 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Fouzia Tarannum ◽  
Mohamed Faizuddin
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Control Group ◽  
Study Group ◽  
Lipoxin A4 ◽  
Disease Pathogenesis ◽  
Clinical Attachment Loss ◽  
Probing Depth ◽  
Crevicular Fluid

Lipoxins play an important role in periodontal resolution, hence, investigation of genetic polymorphism of lipoxin gene may provide important information on the role of lipoxins in periodontal disease pathogenesis. The aim of this study was to investigate a polymorphism of C-to-T substitution at position c.-292 in ALOX15 (reticulocyte-type 15 lipoxygenase 1) gene in patients with chronic periodontitis and to associate the polymorphism with gingival crevicular fluid (GCF) lipoxin A4 (LXA4) levels. Forty-five chronic periodontitis and 45 periodontally healthy patients were included in this case-control study. Plaque index, calculus index, sulcus bleeding index, full mouth probing depth (PD) and clinical attachment loss (CAL) were recorded. GCF and blood samples were collected. GCF was analyzed for LXA4 levels by enzyme linked immunosorbant assay. Genotyping of ALOX15 polymorphism was studied using PCR. Mean LXA4 was lower in periodontitis group compared to the periodontally healthy group. There was a negative correlation between CAL and LXA4. The CC genotype was higher in the study group than in the control group. In the study group, mean CAL was significantly lower among individuals with the CT genotype. Mean LXA4 was significantly lower in CC genotype (45.0±7.11 ng/mL) compared to CT genotype (50.81±5.81 ng/mL) among the patients with periodontitis. The results suggest that LXA4 and c.-292T allele are associated with periodontal health. Polymorphisms in the ALOX15 gene may influence periodontal disease pathogenesis. Hence, investigation of such polymorphisms could benefit the evaluation of lipoxins role in periodontal disease.

scholarly journals Arginine and glutamate levels in the gingival crevicular fluid from patients with chronic periodontitis

2008 ◽  
Vol 19 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Narda Téllez ◽  
Natalia Aguilera ◽  
Belkis Quiñónez ◽  
Elizabeth Silva ◽  
Luis Enrique González ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Healthy Controls ◽  
Clinical Attachment Loss ◽  
Measure Concentration ◽  
Periodontal Tissues ◽  
Glutamate Concentration ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
Post Hoc

The objectives of this study were to determine arginine and glutamate levels in the gingival crevicular fluid (GCF) of adult chronic periodontitis patients versus periodontally healthy controls, and to compare two kinds of microdialysis probes: normal and U-shaped probes. The analysis of GCF components was developed to improve the diagnosis of periodontal disease (PD). Proteolysis in the periodontal tissues increases the concentration of amino acids (aa) in the GCF and the levels of these aa may reveal PD features and stages. GCF samples were collected by microdialysis in situ from 5 periodontally affected sites (probing depth >5 mm, clinical attachment loss >3 mm) in 14 adult chronic periodontitis patients and from 14 adult periodontally healthy controls. Capillary zone electrophoresis coupled to laser induced fluorescence detection was used to measure concentration of arginine and glutamate in the GCF. Data were analyzed statistically by ANOVA and Tukey's post-hoc tests (?=0.05). Arginine concentration was increased (p<0.001) and glutamate concentration was decreased (p<0.001) in chronic periodontitis patients as compared to controls. There were no significant differences (p=0.069) between the normal and U-shaped probes. In conclusion, the increase of arginine and decrease of glutamate concentration in GCF were associated to the presence of periodontitis, and might be used as markers to recognize periodontally susceptible subjects as well as to evaluate the treatment course.

scholarly journals Effectiveness of non-surgical treatment to reduce il-18 levels in the gingival crevicular fluid of patients with periodontal disease

2012 ◽  
Vol 23 (4) ◽  
pp. 428-432 ◽  
Author(s):  
Bernardo Oliveira de Campos ◽  
Ricardo Guimarães Fischer ◽  
Anders Gustafsson ◽  
Carlos Marcelo da Silva Figueredo
Keyword(s):  
Surgical Treatment ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Crevicular Fluid ◽  
Attachment Level ◽  
Gingival Fluid ◽  
Non Surgical Treatment

The aim of this study was to evaluate the effectiveness of the non-surgical periodontal treatment in reducing the gingival crevicular fluid (GCF) levels of IL-18 from inflamed periodontal sites. Fourteen patients with periodontal disease were included, being 9 patients with chronic periodontitis (mean age: 48.8 SD ± 7.4 years) and 5 patients with gingivitis (mean age: 43.6 SD ± 11.8). The patients were divided in the following groups: gingivitis sites from periodontitis patients (sites GP), periodontitis sites from periodontitis patients (sites PP), and gingivitis sites from gingivitis patients (sites GG). Probing pocket depth (PPD), probing attachment level (AL), plaque index (PI) and gingival index (GI) were recorded, and gingival fluid samples were collected. The subjects received non-surgical treatment and were re-evaluated 30 days after treatment (day 30 AT). There was a significant reduction in PI in GG (1.0 ± 0.4 to 0.5 ± 0.2), GP (1.2 ± 0.3 to 0.5 ± 0.3), and in PP (1.3 ± 0.4 to 0.7 ± 0.3) 30 AT. There was also a significant reduction in the GI in GG (1.3 ± 0.3 to 0.7 ± 0.4). PPD reduced significantly in GG (2.4 ± 0.6 to 1.9 ± 0.1), and PP (6.7 ± 1.1 to 5.2 ± 0.9) 30 AT. When all the samples were analyzed together, there was a significant reduction in IL-18 (12.9 ± 7.2 to 10.0 ± 3.1). This study showed that non-surgical treatment was effective in reducing GCF levels of IL-18 from inflamed periodontal sites.

scholarly journals Effect of Periodontal Surgery on Osteoprotegerin Levels in Gingival Crevicular Fluid, Saliva, and Gingival Tissues of Chronic Periodontitis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sandy H. S. Hassan ◽  
Mahmoud I. El-Refai ◽  
Noha A. Ghallab ◽  
Rehab Fawzy Kasem ◽  
Olfat G. Shaker
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Prognostic Biomarker ◽  
Bone Destruction ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Attachment Level

Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF), saliva, and gingival tissues of chronic periodontitis (CP) patients in response to open flap debridement (OFD).Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry.Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P<0.001). Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P<0.001) compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased.Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered withNCT02160613.

Role of Interleukin- 34 together with Receptor Activator of Nuclear Factor kB ligand and Osteoprotegrin levels in Periodontal Disease

2021 ◽  
Vol 15 (7) ◽  
pp. 1521-1522
Author(s):  
Noor Ul Amin ◽  
I. Saleem Qureshi ◽  
Sana Naeem ◽  
S. Hassan Jan ◽  
Kamal Khan ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Control Group ◽  
Gingival Index ◽  
Disease Group ◽  
Clinical Attachment Level ◽  
Group I ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Attachment Level

Aim: To compare the levels and relative ratios of Interleukin-34, osteoprotegrin, and factor kB ligand in the crevicular fluid of individuals with periodontal disease. Study design & setting: Randomized Clinical Trial, Frontier Medical And Dental College, Abbotabad Methodology: We enrolled 15 healthy subjects (Group I), 15 patients with chronic periodontal disease (Group II) and 15 patients with aggressive periodontal disease (Group III). At baseline and six weeks after NSPT, crevicular fluid and clinical measures were made. The GCF IL-34, factor kB ligand, and osteoprotegrin levels were measured by using ELISA and their relative ratios were computed. Result: Group II and III had significantly higher crevicular fluid IL-34 and factor kB ligand levels than the Control group (I), although crevicular fluid with osteoprotegrin levels was significantly decreased (P<0.05). GCF, IL-34, and RANKL levels reduced in Groups II and III six weeks after NSPT, although OPG concentrations increased statistically (P<0.05). Significantly positive association was observed between IL-34 and factor kB ligand, clinical attachment level, and gingival index, but not with osteoprotegrin. Conclusion: Gingival crevicular fluid IL-34 levels were elevated in individuals with periodontal disease and lowered during non surgical intervention, and its levels correlated positively with the factor kB ligand and osteoprotegrin ratios, clinical attachment level, and gingival index. Keywords: Periodontitis, IL-34, osteoprotegrin

scholarly journals Inpatient Evaluation of Periodontal, Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth

2013 ◽  
Vol 14 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Güliz N Güncü ◽  
Gülüzar Büyüktepe ◽  
Sezen B AÕkin ◽  
Kamer Klllnç ◽  
Celal Tümer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Clinical Attachment Level ◽  
Inflammatory Parameters ◽  
Probing Depth ◽  
Natural Teeth ◽  
Fixed Prosthesis ◽  
Crevicular Fluid ◽  
Attachment Level

ABSTRACT Aim The use of endosseous dental implants (DI) has become a successful treatment alternative. However, providing periimplant tissue health and achieving a natural esthetic look are important topics in this treatment. The aim of the present study was to evaluate periodontal and esthetic parameters around DI and natural teeth (NT) and also to analyze myeloperoxidase (MPO) levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF). Materials and methods Twenty DI supported fixed prosthesis and contralateral 20 NT were enrolled to the present study. Clinical periodontal parameters (probing depth, clinical attachment level, gingival bleeding time index and gingival index) were recorded and GCF/PISF samples were obtained from mesial (mesiobuccal and mesiolingual) and distal (distobuccal and distolingual) sites of DI and NT. MPO levels were spectrophotometrically determined. Additionally clinical photographs were obtained and esthetical evaluations were performed by using Jemt papilla index. The parameters belong to DI and NT were compared and correlations were evaluated using statistical analysis. Results A total of 40 samples were evaluated. No statistically significant differences were detected between groups in all periodontal parameters and MPO levels from mesial and distal sites. Jemt papilla index scores were slightly higher in NT however, this difference was not statistically significant (p > 0.05). Total PES score were similiar in DI and NT groups. Significant correlations were detected between MPO and gingival index values as expected. Clinical significance Dental implants are satisfactory treatments, they provide patient esthetic natural looking, phonetic and masticatory functions. How to cite this article Güncü GN, Büyüktepe G, AÕkin SB, KlllnH K, Tümer C, Tözüm TF. Inpatient Evaluation of Periodontal Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth. J Contemp Dent Pract 2013;14(1):90-95.

scholarly journals Alpha-2-Macroglobulin Levels in Gingival Crevicular Fluid Pre- and Post-scaling and Root Planing with Adjunctive Tetracycline Fibers in Chronic Periodontitis: A Randomized Controlled Trial

2015 ◽  
Vol 16 (6) ◽  
pp. 474-478 ◽  
Author(s):  
Shivjot Chhina ◽  
Ajit Singh Rathore ◽  
Saurabh Juneja
Keyword(s):  
Randomized Controlled Trial ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Controlled Trial ◽  
Local Drug Delivery ◽  
Scaling And Root Planing ◽  
Randomized Controlled ◽  
Root Planing ◽  
Probing Depth ◽  
Crevicular Fluid

ABSTRACT Background This split-mouth clinical study aimed to investigate levels of alpha-2-macroglobulin (α2M) in gingival crevicular fluid (GCF) of chronic periodontitis patients pre- and post-scaling and root planing (SRP) with or without adjunctive use of tetracycline fibers. Materials and methods In 30 patients of chronic periodontitis, samples of GCF were collected from the gingival sulcus before SRP. Recording of clinical parameters was conducted. This was followed by local drug delivery (LDD) of tetracycline fibers in test sites. In control sites, no LDD was done. Second samples of GCF were taken 90 days after treatment. Samples of crevicular fluid were analyzed to determine the levels of α2m. Results A gain of clinical attachment (CAL) of 3.30 mm for SRP and LDD and for SRP alone was 1.62 mm (p < 0.001). The pocket probing depth was significantly decreased by 2.43 mm for SRP and LDD and for SRP alone was 1.61 mm (p < 0.001) after 90 days. Alpha-2-macroglobulin was significantly reduced in GCF by SRP and SRP and LDD after 90 days (p < 0.001). Conclusion Clinical and biochemical variables showed a more favorable outcome when SRP was combined with LDD of tetracycline fibers in management of patients suffering from chronic periodontitis. How to cite this article Chhina S, Rathore AS, Juneja S. Alpha-2-Macroglobulin Levels in Gingival Crevicular Fluid Pre- and Post-scaling and Root Planing with Adjunctive Tetracycline Fibers in Chronic Periodontitis: A Randomized Controlled Trial. J Contemp Dent Pract 2015;16(6):474-478.

scholarly journals Estimation of interleukin-17 levels in gingival crevicular fluid from healthy individuals, chronic gingivitis and chronic periodontitis patients using enzyme linked immunosorbent assay

2020 ◽  
Vol 8 (10) ◽  
pp. 3605
Author(s):  
Neha Nainee ◽  
Sheetal Sanikop ◽  
Abhilasha Jha
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Strong Association ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interleukin 17 ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Probing Pocket Depth

Background: Chronic Periodontitis (CP) is an infectious disease resulting in inflammation of supporting tissues of the teeth. A number of pro-inflammatory cytokines are formed against periodontopathogenic microorganisms. Interleukin-17 (IL-17) is a pro-inflammatory cytokine, implicated in numerous inflammatory and autoimmune conditions.Methods: A total of 25 periodontally healthy subjects (Group 1), 25 patients with gingivitis (Group 2) and 25 patients with CP (Group 3) were included for the study based on clinical examination. Gingival index, probing pocket depth and clinical attachment loss were recorded in all subjects.Results: The levels of IL-17 increased from healthy to gingivitis to periodontitis patients. A positive correlation was found with the IL-17 and the clinical parameters like gingival index, probing pocket depth and clinical attachment loss.Conclusions: There is a strong association between the levels of IL-17 with periodontal disease as well as with its severity and its possible use as a biomarker for inflammatory periodontal disease.

scholarly journals Correlation of Levels of Interleukin-1β in Gingival Crevicular Fluid to the Clinical Parameters of Chronic Periodontitis

2011 ◽  
Vol 12 (1) ◽  
pp. 52-59 ◽  
Author(s):  
AU Chaudhari ◽  
GN Byakod ◽  
PF Waghmare ◽  
VM Karhadkar
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Interleukin 1Β ◽  
Clinical Parameters ◽  
Tissue Destruction ◽  
Tooth Mobility ◽  
Crevicular Fluid

ABSTRACT Aim Background and objectives: IL-1β is a potent stimulator of bone resorption and has been implicated in the pathogenesis of periodontal destruction. Therefore, this study was designed to compare the levels of IL-1β of chronic periodontitis patients with the healthy subjects. Another objective of this study was to correlate IL-1β levels with the clinical parameters of the periodontal disease progression. Methods For this study, total 60 subjects were chosen (30- healthy and 30-chronic periodontitis). Simplified oral hygiene index (OHI-S), gingival index (GI), periodontal disease index (PDI), probing depth (PD), tooth mobility, bleeding on probing (BOP) were recorded for all the subject. Gingival crevicular fluid (GCF) was collected and subjected for ELISA for estimation of IL-1β Results At the periodontal diseased sites, the IL-1β levels increased at least 2-fold as compared with healthy subjects. This increase was highly significant (p = 0.0000). Within the test group, IL-1β levels correlated positively and significantly with PDI, PD, BOP and tooth mobility. The correlations of IL-1β with PD (p = 0.000) and IL-1β with BOP (p = 0.0004) were highly significant. Interpretation and conclusion These data suggest that amount of GCF IL-1β is closely associated with periodontal status. This relationship may be valuable in monitoring periodontal disease activity. Clinical significance It could be stated from this study on IL- 1β that there seem to be a strong correlation between periodontal tissue destruction and IL-1β. Furthermore IL-1β level could also differentiate between active and inactive periodontal lesions. How to cite this article Chaudhari AU, Byakod GN, Waghmare PF, Karhadkar VM. Correlation of Levels of Interleukin-1β in Gingival Crevicular Fluid to the Clinical Parameters of Chronic Periodontitis. J Contemp Dent Pract 2011;12(1):52-59.

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