scholarly journals Effect of Heating at Various Temperatures for 30 Minutes on Pathogenicity of White Spot Syndrome Virus (WSSV) in Tiger Prawn (Penaeus monodon Fabr.)

2007 ◽  
Vol 5 (1) ◽  
pp. 5
Author(s):  
D. Priatni ◽  
M. Alifuddin ◽  
D. Djokosetiyanto
Keyword(s):  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Tiger Shrimp ◽  
Pathogenic Virus ◽  
Short Period ◽  
Tiger Prawn ◽  
White Spot Syndrome

<p>White spot syndrome virus<em> </em>(WSSV) is a strong pathogenic virus which spread very rapidly and can cause tiger shrimp mass mortality within a short period.  Enhancement of shrimp immunity by infecting inactivated WSSV is one of the efforts to overcome WSSV infection in shrimp.  In this study, inactivated WSSV were prepared by heating them with various temperatures namely 45, 50, 55 and 60<sup>o</sup>C for 30 minutes. The results shows that infection with  heating inactivated WSSV at 45°C and 60°C for 30 min on PL-15 could increase their immunities.  The survival rate of inactivated WSSV-infected shrimp after challenge test with  WSSV virulent  reached 77%, while  no survive shrimp was observed in control.  This suggests that shrimp immunity could be improved by vaccination using  WSSV virus inactivated by heating.</p> <p>Keywords: WSSV virus, pathogen, tiger shrimp, heating</p> <p> </p> <p>ABSTRAK</p> <p><em>White Spot Syndrome Virus </em>(WSSV) merupakan virus yang sangat ganas bagi udang windu, dengan penularan yang sangat cepat dan menyebabkan kematian dalam waktu yang cepat. Peningkatan imunitas udang dengan meenginfeksikan WSSV inaktif merupakan salah satu upaya untuk menanggulangi infeksi WSSV pada udang. Pada penelitian ini, inaktivasi WSSV dilakukan menggunakan pemanasan pada suhu berbeda, yaitu 45, 50, 55 dan 60<sup>o</sup>C selama 30 menit.  Hasil penelitian menunjukkan bahwa infeksi PL-15 menggunakan virus WSSV yang telah diinaktivasi dengan pemanasan pada suhu 45<sup>o</sup>C dan 60<sup>o</sup>C dapat meningkatkan daya tahan udang.  Kelangsungan hidup udang yang telah diinfeksi dengan WSSV hasil inaktivasi sebelum uji tantang dengan WSSV virulen mencapai 77%, sementara udang yang tidak diinfeksi dengan WSSV hasil inaktivasi adalah semua mati.  Dengan demikian dapat disimpulkan bahwa daya tahan udang dapat ditingkatkan melalui vaksinasi menggunakan WSSV yang telah diinaktivasi dengan pemanasan.</p> <p>Kata kunci: virus WSSV, patogen, udang windu, pemanasan</p>

scholarly journals Effect of Ultraviolet Radiation on Pathogenity of White Spot Syndrome Virus in Giant Tiger Prawn (Penaeus monodon Fab.)

2007 ◽  
Vol 4 (1) ◽  
pp. 79
Author(s):  
M. Subkhan ◽  
M. Alifuddin ◽  
A. Taslihan
Keyword(s):  
Uv Irradiation ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Virus Suspension ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Tiger Prawn ◽  
White Spot Syndrome

<p>This experiment was conducted to examine the effect of UV irradiation on pathogenity of white spot syndrome virus (WSSV) in black tiger shrimp (<em>Penaeus monodon</em> Fab.).  A hundred ml of WSSV virus suspension (200 μg/ml) were placed at 30 cm under UV light 10 Watt.  Radiation on WSSV virus was performed for 15, 30, 45, and 60 minutes.  Black tiger sjrimp in density of 260 tails/L were immersed in 1000 ml of irradiated virus suspension (20 μg/ml) to test their pathogenities.  The results of study showed that duration of UV irradiation on WSSV virus was reverse correlated to their pathogenities.  In constrast, survival of black tiger shrimp was linear correlated to duration of UV inactivation of virus.  Higher survival rate of shrimp (65.52%) after challenge test was obtained by irradiation of virus for 60 min.</p> <p>Keywords:  WSSV, virus, pathogen, ultraviolet, black tiger prawn, <em>Penaeus monodon</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui pengaruh radiasi UV terhadap patogenitas virus <em>White Spot Syndrome Virus</em> (WSSV) pada udang windu (<em>Penaeus monodon </em>Fab).   Suspensi virus WSSV dengan konsentrasi 200 μg/ml sebanyak 100 ml ditempatkan 30 cm diradiasi menggunakan UV 10 Watt.  Radiasi dilakukan selama 15, 30, 45, dan 60 menit.  Udang dengan kepadatan 260 ekor/L direndam dalam 1000 ml suspensi virus (20 μg/ml) hasil radiasi untuk menguji patogenitasnya.  Hasil penelitian menunjukkan bahwa lama radiasi UV pada virus WSSV berbanding terbalik dengan tingkat patogenitasnya.  Sementara itu, lama inaktivasi virus dengan UV berbanding lurus terhadap kelangsungan hidup udang.  Kelangsungan hidup udang windu tertinggi (65,52%) setelah uji tantang diperoleh dengan meradiasi virus selama 60 menit.</p> <p>Kata kunci: WSSV, virus, patogen, ultraviolet, udang windu,  <em>Penaeus monodon</em></p>

scholarly journals Enhancement of Tiger Shrimp Penaeus monodon Resistance to White Spot Syndrome Virus by Overexpression of Antiviral Gene

2021 ◽  
Vol 25 (02) ◽  
pp. 277-284
Author(s):  
Andi Parenrengi
Keyword(s):  
Gene Expression ◽  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Tiger Shrimp ◽  
Significant Difference ◽  
White Spot Syndrome ◽  
Antiviral Gene

White spot syndrome virus (WSSV) is a significant cause of tiger shrimp mortality, and has a profound economic impact on commercial shrimp farming. A transgenic technology provides an effective method to control the viral disease by increasing the shrimp resistance. The study aimed to assess the overexpression of the PmAV (Penaeus monodon antiviral) gene on the embryo/larvae of tiger shrimp and the performance of transgenic shrimp by WSSV-challenge test. Transfection of pProAV-PmAV gene construct into the embryo was conducted using jetPEI reagent. Transient antiviral gene expression was detected at 12, 18, 24 and 30 h post transfection (hpt). The tiger shrimp larvae of PL-25 were challenged by waterborne infection technique. Survival and expression of antiviral genes were observed after challenge test. The results showed that the PmAV gene and the jetPEI reagent did not have the toxicity to the tiger shrimp larvae. The transient antiviral gene expression was exhibited at 12 hpt until the peak reached within 24 hpt and then decreased slightly at 30 hpt. This suggested that the expression of PmAV during infection was an up-regulated sign. The challenge test result showed that the transgenic tiger shrimp exhibited higher resistance (P < 0.05) to the WSSV infection (95.6% survival rate) than the control shrimp (71.1% survival rate). The 47-day transgenic tiger shrimp did not show significant difference (P > 0.05) in body weight and length gain as well as appearance, morphology, and activity from normal control shrimps. © 2021 Friends Science Publishers

scholarly journals Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon

2008 ◽  
Vol 79 ◽  
pp. 157-161 ◽  
Author(s):  
R Patil ◽  
KJ Palaksha ◽  
TM Anil ◽  
Guruchannabasavanna ◽  
P Patil ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Tiger Shrimp ◽  
White Spot Syndrome

scholarly journals Persistence of Penaeus stylirostris densovirus delays mortality caused by white spot syndrome virus infection in black tiger shrimp (Penaeus monodon)

2013 ◽  
Vol 9 (1) ◽  
pp. 33 ◽  
Author(s):  
Sudkhate Molthathong ◽  
Sarocha Jitrakorn ◽  
Yutthana Joyjinda ◽  
Chuenchit Boonchird ◽  
Boonsirm Witchayachamnarnkul ◽  
...  
Keyword(s):  
Virus Infection ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
White Spot Syndrome ◽  
Penaeus Stylirostris Densovirus

scholarly journals PmVRP15, a Novel Viral Responsive Protein from the Black Tiger Shrimp, Penaeus monodon, Promoted White Spot Syndrome Virus Replication

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91930 ◽  
Author(s):  
Tipachai Vatanavicharn ◽  
Adisak Prapavorarat ◽  
Phattarunda Jaree ◽  
Kunlaya Somboonwiwat ◽  
Anchalee Tassanakajon
Keyword(s):  
Virus Replication ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
White Spot Syndrome
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