The use of Bacillus spp. as bacterial biocontrol agents to control plant diseases

2021 ◽  
Author(s):  
Adrien Anckaert ◽  
◽  
Anthony Arguelles Arias ◽  
Grégory Hoff ◽  
Maryline Calonne-Salmon ◽  
...  
Keyword(s):  
Control Plant ◽  
Ecological Factors ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Biocontrol Agents ◽  
Systemic Resistance ◽  
Health Improvement ◽  
Wide Range ◽  
Bacillus Spp ◽  
Plant Growth Promoting

Biocontrol agents (BCAs) based on plant growth promoting rhizobacteria have recently been developed as alternatives to chemical pesticides. Among those beneficial bacteria, Bacillus spp. are one of the most promising BCAs. A wide range of bioactive secondary metabolites (BSMs) are involved in biocontrol via antibiosis to phytopathogens and/or via elicitation of systemic resistance in their host plants. This chapter illustrates the diversity of pathosystems in which BCA based on Bacillus spp. have proved effective. It describes the mechanisms underpinning this biocontrol activity via production of a wide range of enzymes, proteins and small-size BSMs. As these BSMs are clearly involved in pathogen control, we emphasise the importance of understanding the ecological factors influencing their production. In the last part of the chapter, we highlight the potential interactions between Bacillus spp. and other soil microorganisms in developing consortia of biocontrol agents combining species with synergistic activities for plant health improvement.

scholarly journals The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020 ◽  
Vol 8 (7) ◽  
pp. 1037 ◽  
Author(s):  
Dragana Miljaković ◽  
Jelena Marinković ◽  
Svetlana Balešević-Tubić
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Biocontrol Agents ◽  
Systemic Resistance ◽  
Steady Increase ◽  
Vegetable Crops ◽  
Wide Range ◽  
Bacillus Spp

Bacillus spp. produce a variety of compounds involved in the biocontrol of plant pathogens and promotion of plant growth, which makes them potential candidates for most agricultural and biotechnological applications. Bacilli exhibit antagonistic activity by excreting extracellular metabolites such as antibiotics, cell wall hydrolases, and siderophores. Additionally, Bacillus spp. improve plant response to pathogen attack by triggering induced systemic resistance (ISR). Besides being the most promising biocontrol agents, Bacillus spp. promote plant growth via nitrogen fixation, phosphate solubilization, and phytohormone production. Antagonistic and plant growth-promoting strains of Bacillus spp. might be useful in formulating new preparations. Numerous studies of a wide range of plant species revealed a steady increase in the number of Bacillus spp. identified as potential biocontrol agents and plant growth promoters. Among different mechanisms of action, it remains unclear which individual or combined traits could be used as predictors in the selection of the best strains for crop productivity improvement. Due to numerous factors that influence the successful application of Bacillus spp., it is necessary to understand how different strains function in biological control and plant growth promotion, and distinctly define the factors that contribute to their more efficient use in the field.

scholarly journals Diffusible and Volatile Antifungal Compounds Produced by Pseudomonas chlororaphis subsp. aurantiaca ST-TJ4 against Various Phytopathogenic Fungi

2020 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun
Keyword(s):  
Control Plant ◽  
Pathogenic Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Pseudomonas Chlororaphis ◽  
Headspace Collection ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting

Abstract Plant growth-promoting rhizobacteria can potentially be used as an alternative strategy to control plant disease. In this study, strain ST-TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST-TJ4 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on 16S rDNA sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1-phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Moreover, the volatile compounds released by strain ST-TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST-TJ4 obtained from headspace collection and GC-MS/MS analysis, 1-undecene was identified. In summary, the results suggested that P. chlororaphis subsp. aurantiaca ST-TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

scholarly journals Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi

2020 ◽  
Vol 8 (4) ◽  
pp. 590 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun
Keyword(s):  
Control Plant ◽  
Pathogenic Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Forest Tree ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Pseudomonas Sp ◽  
Headspace Collection ◽  
Plant Growth Promoting ◽  
Encoding Gene

Plant growth-promoting rhizobacteria (PGPR) can potentially be used as an alternative strategy to control plant diseases. In this study, strain ST–TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have a strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST–TJ4 was identified as Pseudomonas sp. based on 16S rRNA-encoding gene sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1–phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Additionally, the volatile compounds released by strain ST–TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST–TJ4 obtained from headspace collection and GC–MS/MS analysis, 1-undecene was identified. In summary, the results suggested that Pseudomonas sp. ST–TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

scholarly journals NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN

Agric ◽  
2018 ◽  
Vol 30 (1) ◽  
pp. 25-32
Author(s):  
Reginawanti Hindersah ◽  
Marthin Kalay ◽  
Abraham Talahaturuson ◽  
Yansen Lakburlawal
Keyword(s):  
Block Design ◽  
Control Plant ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Damping Off ◽  
Randomized Block Design ◽  
Plant Growth Promoting ◽  
Npk Fertilizer ◽  
Growth Promoting ◽  
And Control

Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.

scholarly journals C4 Bacterial Volatiles Improve Plant Health

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 682
Author(s):  
Bruno Henrique Silva Dias ◽  
Sung-Hee Jung ◽  
Juliana Velasco de Castro Oliveira ◽  
Choong-Min Ryu
Keyword(s):  
Plant Growth ◽  
Volatile Compounds ◽  
Large Scale ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Health ◽  
Systemic Resistance ◽  
Potential Applications ◽  
Plant Growth Promoting ◽  
Bacterial Volatiles

Plant growth-promoting rhizobacteria (PGPR) associated with plant roots can trigger plant growth promotion and induced systemic resistance. Several bacterial determinants including cell-wall components and secreted compounds have been identified to date. Here, we review a group of low-molecular-weight volatile compounds released by PGPR, which improve plant health, mostly by protecting plants against pathogen attack under greenhouse and field conditions. We particularly focus on C4 bacterial volatile compounds (BVCs), such as 2,3-butanediol and acetoin, which have been shown to activate the plant immune response and to promote plant growth at the molecular level as well as in large-scale field applications. We also disc/ uss the potential applications, metabolic engineering, and large-scale fermentation of C4 BVCs. The C4 bacterial volatiles act as airborne signals and therefore represent a new type of biocontrol agent. Further advances in the encapsulation procedure, together with the development of standards and guidelines, will promote the application of C4 volatiles in the field.

scholarly journals Activity of plant growth promoting rhizobacteria (PGPRs) in the biocontrol of tomato Fusarium wilt

2017 ◽  
Vol 53 (No. 2) ◽  
pp. 78-84 ◽  
Author(s):  
Boukerma Lamia ◽  
Benchabane Messaoud ◽  
Charif Ahmed ◽  
Khélif Lakhdar
Keyword(s):  
Fusarium Wilt ◽  
Plant Growth Promoting Rhizobacteria ◽  
Significant Inhibition ◽  
Systemic Resistance ◽  
In Situ Experiment ◽  
Fluorescent Pseudomonas ◽  
Split Root ◽  
Plant Growth Promoting ◽  
Severity Of The Disease

The potential of Pseudomonas fluorescens PF15 and Pseudomonas putida PP27 to protect tomato plants against Fusarium wilt under greenhouse conditions was evaluated. In vitro antagonism showed a significant inhibition of the pathogen growth (47%) revealed by PF15. However, PP27 presented a 10% rate of the mycelium inhibition. An in situ experiment was conducted with split-root design for induced systemic resistance (ISR) and without split-root design to measure both ISR and antagonistic activities. Fluorescent Pseudomonas revealed a delay in the onset of symptoms and slower kinetics of disease progression compared to the pathogen control. McKinney’s index, which measures the severity of the disease, was reduced by 37–72%, and the levels of infection (incidence) by 7–36%.

scholarly journals Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

2021 ◽  
Vol 9 (7) ◽  
pp. 1441
Author(s):  
Van Bach Lam ◽  
Thibault Meyer ◽  
Anthony Arguelles Arias ◽  
Marc Ongena ◽  
Feyisara Eyiwumi Oni ◽  
...  
Keyword(s):  
Rice Blast ◽  
Induced Systemic Resistance ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Systemic Resistance ◽  
Acid Sulfate Soils ◽  
Acid Sulfate ◽  
Wide Range ◽  
Bacillus Spp ◽  
Sulfate Soils

Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.

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